{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:38:57Z","timestamp":1747186737804,"version":"3.40.5"},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Small Animal Practice"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To develop and determine the accuracy of a rapid manual technique for the detection of pre\u2010treatment neutropenia (&lt;1.50\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\/L) in dogs receiving chemotherapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Twenty canine blood smears with known neutrophil counts between 1.00\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\/L and 3.00\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\/L were reviewed by two internal medicine clinicians and linear regressions performed to determine a cut\u2010off value for a manual neutrophil count equating to &gt;1.50\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\/L. Consecutive blood samples from dogs undergoing chemotherapy were processed through an automated haematology analyser (VetScan HM5, Abaxis), and prospective blinded manual review by the same two observers assessed whether the manual technique could accurately detect dogs with neutropenia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Linear regression analysis found a cut\u2010off of &gt;26 neutrophils per 10 low power fields at the monolayer to be equivalent to a neutrophil count of &gt;1.5\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\/L. A total of 183 blood samples from 43 dogs were reviewed. Automated techniques detected neutropenia in 16 of 183 (9%) blood samples. Using the manual cut\u2010off technique, 13 of 16 (81%) and 11 of 16 (69%) of neutropenic samples were correctly identified by observer 1 and observer 2, respectively. Twenty\u2010three of 167 non\u2010neutropenic dogs (14%) were incorrectly classified as neutropenic by observer 1, and 27 (16%) by observer 2. Inter\u2010observer agreement was 92%. Sensitivity was 81% (95% confidence interval 54% to 96%) for observer 1 and 69% (95% confidence interval 41% to 89%) for observer 2. Specificity was 86% (95% confidence interval 80% to 91%) for observer 1 and 84% (95% confidence interval 77% to 89%) for observer 2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical Significance<\/jats:title><jats:p>Manual estimation resulted in up to five of 16 (31%) neutropenic samples being incorrectly classified. A full automated differential cell count remains preferable.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jsap.13773","type":"journal-article","created":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T01:40:33Z","timestamp":1722994833000},"page":"849-856","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A rapid manual technique does not have adequate sensitivity and specificity to reliably discriminate between neutropenic and non\u2010neutropenic dogs prior to administration of chemotherapy"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8975-0179","authenticated-orcid":false,"given":"M.","family":"Walton\u2010Clark","sequence":"first","affiliation":[{"name":"Eastcott Referrals, Part of Linnaeus Veterinary Limited, Edison Business Park  Swindon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Henriques","sequence":"additional","affiliation":[{"name":"Eastcott Referrals, Part of Linnaeus Veterinary Limited, Edison Business Park  Swindon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Best","sequence":"additional","affiliation":[{"name":"Eastcott Referrals, Part of Linnaeus Veterinary Limited, Edison Business Park  Swindon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2006.10.002"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2008.00085.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00282-999-0211-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.242.11.1539"},{"key":"e_1_2_6_6_1","first-page":"1144","volume-title":"Small animal internal medicine","author":"Couto C.G.","year":"2014"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.2003.tb00103.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2009.00110.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.14895"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.5045\/br.2014.49.2.120"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2010.00280.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.21037\/cdt.2017.03.12"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.5326\/0430001"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.1988.tb00477.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.tcam.2009.02.004"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-53045-3.00022-2"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119380528.ch14c"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.04.032"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2002.220.1805"}],"container-title":["Journal of Small Animal Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jsap.13773","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T08:38:30Z","timestamp":1733128710000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jsap.13773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,6]]},"references-count":18,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/jsap.13773"],"URL":"https:\/\/doi.org\/10.1111\/jsap.13773","archive":["Portico"],"relation":{},"ISSN":["0022-4510","1748-5827"],"issn-type":[{"type":"print","value":"0022-4510"},{"type":"electronic","value":"1748-5827"}],"subject":[],"published":{"date-parts":[[2024,8,6]]},"assertion":[{"value":"2023-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}