{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T00:58:19Z","timestamp":1775437099632,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Small Animal Practice"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To assess topical ethyl alcohol as a novel treatment for canine superficial pyoderma and compare its effects with chlorhexidine digluconate on the relative abundance of\n                      <jats:italic>Staphylococcus pseudintermedius<\/jats:italic>\n                      and\n                      <jats:italic>Staphylococcus schleiferi<\/jats:italic>\n                      within the skin microbiome.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      In this prospective, randomised, blinded study, 15 client\u2010owned dogs with bilaterally symmetric bacterial skin lesions received twice\u2010daily topical chlorhexidine digluconate 3% (Douxo\u2122 S3 Pyo mousse, CEVA; Libourne, France) or 70% ethyl alcohol (Purell\u2122 Gel, GOJO Industries; Wooster, OH, USA) for 28\u2009days. Lesions were clinically and cytologically scored at days 0, 14 and 28 using a modified split\u2010body system to generate a global score. Next\u2010generation DNA sequencing characterised the\n                      <jats:italic>S. pseudintermedius<\/jats:italic>\n                      and\n                      <jats:italic>S. schleiferi<\/jats:italic>\n                      loads at day 0 and 28.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Both treatments significant improved global scores from baseline (chlorhexidine 18.22 \u00b1\u20093.54; ethyl alcohol 17.95 \u00b1\u20093.78) at 14\u2010days (chlorhexidine 12.80 \u00b1\u20092.74,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u20090.001; ethyl alcohol 12.40 \u00b1\u20093.06,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u20090.001) and 28\u2010days (chlorhexidine 9.24 \u00b1\u20093.57,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u20090.001 and ethyl alcohol 8.40 \u00b1\u20094.06,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u20090.001). Global scores were not significantly different between treatment groups at any time point. Both treatments also reduced the relative abundance of\n                      <jats:italic>S. pseudintermedius<\/jats:italic>\n                      (chlorhexidine \u221228.73% \u00b1\u200942.86%; ethyl alcohol \u221225.66% \u00b1\u200938.74%) and\n                      <jats:italic>S. schleiferi<\/jats:italic>\n                      (chlorhexidine \u221265.93% \u00b1\u200913.03%; ethyl alcohol \u221279.82% \u00b1\u20098.37%). No statistically significant differences were observed in the relative abundance of either species between the topical treatments (\n                      <jats:italic>p<\/jats:italic>\n                      =0.95\n                      <jats:italic>S. pseudintermedius<\/jats:italic>\n                      ;\n                      <jats:italic>p<\/jats:italic>\n                      =0.38\n                      <jats:italic>S. schleiferi<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Significance<\/jats:title>\n                    <jats:p>This study suggests topical 70% ethyl alcohol may be an effective treatment for canine superficial pyoderma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jsap.70068","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T01:14:58Z","timestamp":1766020498000},"page":"359-367","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Topical ethyl alcohol as a novel treatment for superficial bacterial pyoderma in dogs"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5303-8066","authenticated-orcid":false,"given":"K.","family":"Masutani","sequence":"first","affiliation":[{"name":"Animal Dermatology Clinic  Lynwood Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8284-9881","authenticated-orcid":false,"given":"J. E.","family":"Brune","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine University of Washington  Seattle Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4077-1048","authenticated-orcid":false,"given":"D.","family":"Duclos","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic  Lynwood Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6113-9394","authenticated-orcid":false,"given":"N.","family":"Rich","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic  Lynwood Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofx054"},{"key":"e_1_2_8_3_1","first-page":"204","article-title":"Canine superficial pyoderma and therapeutic 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