{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:50:59Z","timestamp":1791571859580,"version":"4.3.4"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Small Animal Practice"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To characterise multidrug antimicrobial resistance and pathogenicity profiles of bacteria isolated from different anatomical sites in bitches with pyometra, emphasising their clinical relevance for diagnosis and therapeutic decision\u2010making in small animal practice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Bacteria were obtained from the faeces, urine, uterus and vagina of 23 dogs diagnosed with pyometra undergoing ovariohysterectomy. Antimicrobial resistance profiles were analysed for all identified isolates, together with phylogenetic classification of\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      and detection of virulence factor\u2013encoding genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Bacterial isolates obtained from faecal, urinary, uterine and vaginal samples showed predominantly positive growth for\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      , followed by\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      spp.,\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      and\n                      <jats:italic>Proteus<\/jats:italic>\n                      spp. Among these isolates recovered from each anatomical site, multidrug\u2010resistant bacteria \u2013 defined as resistance to three or more classes of antimicrobials \u2013 were identified across all bacterial genera, with frequencies of 37.7% among faecal isolates, 16.7% among urinary isolates, 44.5% among uterine isolates and 100% among vaginal isolates, except for\n                      <jats:italic>Enterobacter<\/jats:italic>\n                      spp. Among dogs positive for\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      (11\/23), phylogroups A\/C (30.9%) were the most prevalent, followed by D\/E (16.9%), B2 (15.5%), B1 (12.7%), A (12.7%), C (8.5%) and U (2.8%). In 90% (10\/11) of cases,\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      isolates recovered from faecal, vaginal and\/or uterine samples belonged to the same clonal family, suggesting faecal contamination. Overall, 77.4% of\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      isolates of uterine origin were classified as resistant or multidrug\u2010resistant, and 83.9% harboured one or more virulence factor\u2010encoding genes (hlyA, uspA, papC and predominantly fimH). Phylogroup B2 was the only group in which all assessed virulence factors were simultaneously detected.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Significance<\/jats:title>\n                    <jats:p>\n                      The presence of multidrug\u2010resistant bacteria across all genera isolated in cases of canine pyometra underscores the clinical importance of antimicrobial susceptibility testing. Furthermore, the recovery of genetically related\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      isolates from intestinal and reproductive tract samples supports the intestinal microbiota as a relevant source of infection in affected bitches.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jsap.70110","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T07:25:25Z","timestamp":1773645925000},"page":"627-634","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Multidrug resistance of bacterial pathogens in canine pyometra"],"prefix":"10.1111","volume":"67","author":[{"given":"M. G. M.","family":"Camozzi","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Bioci\u00eancia e Sa\u00fade \u00danica (PPGBSU), Instituto de Ci\u00eancias Agr\u00e1rias (ICA) Universidade Federal de Jata\u00ed (UFJ)  Jata\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6719-1794","authenticated-orcid":false,"given":"S. B.","family":"Waller","sequence":"additional","affiliation":[{"name":"N\u00facleo de Biotecnologia Centro de Desenvolvimento Tecnol\u00f3gico (CDTec), Universidade Federal de Pelotas (UFPel)  Pelotas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. C.","family":"Castelhano","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Jata\u00ed UFJ  Jata\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. L. S.","family":"Neves","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias da Sa\u00fade Universidade Federal de Jata\u00ed UFJ  Jata\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. R.","family":"Arrais","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Bioci\u00eancia e Sa\u00fade \u00danica (PPGBSU), Instituto de Ci\u00eancias Agr\u00e1rias (ICA) Universidade Federal de Jata\u00ed (UFJ)  Jata\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. F. N.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Jata\u00ed UFJ  Jata\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9465-8054","authenticated-orcid":false,"given":"B. G.","family":"Alves","sequence":"additional","affiliation":[{"name":"Conception Biosciences Inc.  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