{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:22:39Z","timestamp":1788434559242,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100008015","name":"Agria Djurf\u00f6rs\u00e4kring","doi-asserted-by":"publisher","award":["N2018\u20100007"],"award-info":[{"award-number":["N2018\u20100007"]}],"id":[{"id":"10.13039\/501100008015","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Small Animal Practice"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate the discriminatory potential of serum amyloid A, neutrophil:lymphocyte ratio, monocyte:lymphocyte ratio and assess renal tubular injury in cats with pyelonephritis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Diagnostic accuracy study using samples from prospectively enrolled cats and stored material. Cats were classified according to diagnosis as pyelonephritis, ureteral obstruction, chronic kidney disease, subclinical bacteriuria, cystitis, or healthy. Serum amyloid A, neutrophil:lymphocyte ratio, monocyte:lymphocyte ratio and urinary kidney injury marker\u20101 were compared across groups. Receiver operating characteristic curve analysis assessed the performance of inflammatory biomarkers. Serum amyloid A and urinary kidney injury marker\u20101 were measured consecutively in a subset of cats with pyelonephritis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Samples from 71 cats (pyelonephritis\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200922, other urological conditions\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200935, healthy\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200914) were included. Median serum amyloid A concentration was significantly higher in cats with pyelonephritis, overlapping minimally with other groups. At a threshold of 49.1\u2009mg\/L, serum amyloid A showed a positive likelihood ratio of 32, sensitivity of 0.95 (95% CI: 0.74 to 0.99) and specificity of 0.97 (95% CI: 0.85 to 0.99), outperforming neutrophil:lymphocyte ratio. Serum amyloid A normalised in 12\/14 cats post\u2010treatment. Urinary kidney injury marker\u20101 was elevated in more than 50% of cats with pyelonephritis and ureteral obstruction, significantly higher than in healthy cats and those with subclinical bacteriuria or cystitis. Variation in urinary kidney injury marker\u20101 was observed during follow\u2010up.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>At a threshold of 49\u2009mg\/L, serum amyloid A is a highly accurate biomarker for differentiating pyelonephritis from other urological conditions. Elevated urinary kidney injury marker\u20101 suggests frequent proximal tubular injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Significance<\/jats:title>\n                    <jats:p>Pyelonephritis is challenging to diagnose. Serum amyloid A can improve diagnostic precision and support timely treatment decisions. Urinary kidney injury marker\u20101 helps identify tubular injury; however, the clinical implications remain to be investigated.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jsap.70140","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T06:28:08Z","timestamp":1779690488000},"page":"810-821","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Increased serum amyloid A and urinary kidney injury marker\u20101 concentrations in cats with pyelonephritis: a diagnostic accuracy study"],"prefix":"10.1111","volume":"67","author":[{"given":"L. R.","family":"Jessen","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Copenhagen  Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. S.","family":"Moberg","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Copenhagen  Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Langhorn","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Copenhagen  Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S.","family":"Gravgaard","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Copenhagen  Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Dupont","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Copenhagen  Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Bak\u2010Jacobsen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Copenhagen  Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. J.","family":"Reezigt","sequence":"additional","affiliation":[{"name":"Blue Star Veterinary Hospital  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. L.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Animal and Veterinary Sciences University of Aarhus  Tjele Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Bienzle","sequence":"additional","affiliation":[{"name":"Department of Pathobiology Ontario Veterinary College, University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3988-0979","authenticated-orcid":false,"given":"L. N.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Copenhagen  Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X18812494"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12428"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985817690213"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2005.tb00019.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2023.1673-1681"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13162579"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0270067"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11061644"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.14661"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15628"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X251314778"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.17082"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X18813427"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.13238"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.65.545"},{"key":"e_1_2_9_17_1","first-page":"625","article-title":"Urinary neutrophil gelatinase\u2010associated lipocalin, kidney injury molecule\u20101, uromodulin, and cystatin C concentrations in an experimental rat model of ascending acute kidney injury induced by pyelonephritis","volume":"69","author":"Skowron B.","year":"2018","journal-title":"Journal of Physiology and Pharmacology"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638717722815"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-022-03456-5"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2019.02.008"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.23.08.0488"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci7020045"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5002697"}],"container-title":["Journal of Small Animal Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jsap.70140","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jsap.70140","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jsap.70140","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:10:46Z","timestamp":1788433846000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jsap.70140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,24]]},"references-count":22,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/jsap.70140"],"URL":"https:\/\/doi.org\/10.1111\/jsap.70140","archive":["Portico"],"relation":{},"ISSN":["0022-4510","1748-5827"],"issn-type":[{"value":"0022-4510","type":"print"},{"value":"1748-5827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,24]]},"assertion":[{"value":"2025-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}