{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:02:33Z","timestamp":1781071353196,"version":"3.54.1"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100015900","name":"OVC Pet Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015900","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Small Animal Practice"],"abstract":"\n Objectives<\/jats:title>\n It is recommended that all cats, regardless of transfusion history, undergo major crossmatch prior to blood product administration to decrease the likelihood of immunological transfusion reactions. A reliable and user\u2010friendly point\u2010of\u2010care crossmatch test would help make crossmatch testing more accessible. The primary objective of this study was to assess agreement between two antiglobulin\u2010enhanced major crossmatch test methods, a laboratory\u2010based tube crossmatch method and a point\u2010of\u2010care gel column crossmatch method, in cats in need of blood transfusion.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n Cats that had the potential to require blood transfusion were prospectively recruited. Each cat underwent major crossmatch using two methods concurrently: a laboratory\u2010based tube crossmatch method performed by a reference laboratory, and a gel column crossmatch method performed in the clinic by a clinician and interpreted using guidelines supplied by the manufacturer.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n A total of 95 unique donor\u2010recipient pairings were evaluated with both major crossmatch methods. Of the 95 pairings, 21 were incompatible using the laboratory\u2010based tube crossmatch test while 18 were incompatible on the gel column crossmatch test; 13 pairings were incompatible on both methods. Kappa agreement between the laboratory\u2010based tube crossmatch and gel column crossmatch methods was 0.581 (95% confidence interval: 0.378 to 0.785), showing significant agreement. The overall percent agreement between the tests was 86.3%; positive percent agreement was 93.2%, while negative percent agreement was 61.9%.<\/jats:p>\n <\/jats:sec>\n \n Clinical Significance<\/jats:title>\n The commercial antiglobulin\u2010enhanced, point\u2010of\u2010care gel major crossmatch test had moderate agreement with the antiglobulin\u2010enhanced tube crossmatch performed at a reference laboratory. Inclusion of larger numbers of incompatible pairings would help further evaluate the agreement between the two test methods.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jsap.70152","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T05:26:08Z","timestamp":1781069168000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of point\u2010of\u2010care\n versus<\/i>\n laboratory antiglobulin\u2010enhanced major crossmatch testing in cats"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4148-4617","authenticated-orcid":false,"given":"S. L.","family":"Blois","sequence":"first","affiliation":[{"name":"Department of Clinical Studies Ontario Veterinary College, University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J.","family":"Collier","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies Ontario Veterinary College, University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sanz","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies Ontario Veterinary College, University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Chun","sequence":"additional","affiliation":[{"name":"Health Sciences Centre, Ontario Veterinary College, University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1111\/jvim.16010","article-title":"Identification of 5 novel feline erythrocyte antigens based on the presence of naturally occurring alloantibodies","volume":"35","author":"Binvel M.","year":"2021","journal-title":"Journal of Veterinary Internal Medicine"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.tvjl.2021.105663","article-title":"Accuracy of a point\u2010of\u2010care major crossmatch test and risk factors for major crossmatch incompatibility in cats","volume":"272","author":"Blois S.L.","year":"2021","journal-title":"The Veterinary Journal"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0165-2427(93)90088-L","article-title":"Alloantibodies against A and B blood types in cats","volume":"38","author":"B\u00fccheler J.","year":"1993","journal-title":"Veterinary Immunology and Immunopathology"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"e124","DOI":"10.1177\/1098612X221094502","article-title":"Characterization of post\u2010transfusion anti\u2010FEA 1 alloantibodies in transfusion\u2010naive FEA 1\u2010negative cats","volume":"24","author":"Cannavino A.","year":"2022","journal-title":"Journal of Feline Medicine and Surgery"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/vec.13045","article-title":"Association of Veterinary Hematology and Transfusion Medicine (AVHTM) Transfusion Reaction Small Animal Consensus Statement (TRACS) Part 2: prevention and monitoring","volume":"31","author":"Davidow E.B.","year":"2021","journal-title":"Journal of Veterinary Emergency and Critical Care"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/vec.13044","article-title":"Association of Veterinary Hematology and Transfusion Medicine (AVHTM) Transfusion Reaction Small Animal Consensus Statement (TRACS). Part 1: definitions and clinical signs","volume":"31","author":"Davidow E.B.","year":"2021","journal-title":"Journal of Veterinary Emergency and Critical Care"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1111\/vcp.70039","article-title":"Comparison of three feline crossmatch methods\u2014tube, gel column, and immunochromatographic strip","volume":"54","author":"DiCiccio V.K.","year":"2025","journal-title":"Veterinary Clinical Pathology"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/j.1939-1676.1990.tb03129.x","article-title":"Acute hemolytic transfusion reaction in an Abyssinian cat with blood type B","volume":"4","author":"Giger U.","year":"1990","journal-title":"Journal of Veterinary Internal Medicine"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1111\/jvim.14801","article-title":"Pre\u2010 and post\u2010transfusion alloimmunization in dogs characterized by 2 antiglobulin\u2010enhanced cross\u2010match tests","volume":"31","author":"Goy\u2010Thollot I.","year":"2017","journal-title":"Journal of Veterinary Internal Medicine"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1111\/jvim.15381","article-title":"Detection of naturally occurring alloantibody by an in\u2010clinic antiglobulin\u2010enhanced and standard crossmatch gel column test in non\u2010transfused domestic shorthair cats","volume":"33","author":"Goy\u2010Thollot I.","year":"2019","journal-title":"Journal of Veterinary Internal Medicine"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1111\/vec.12433","article-title":"Comparison of a commercial blood cross\u2010matching kit to the standard laboratory method for establishing blood transfusion compatibility in dogs","volume":"26","author":"Guzman L.R.","year":"2016","journal-title":"Journal of Veterinary Emergency and Critical 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outcomes","volume":"32","author":"McClosky M.E.","year":"2018","journal-title":"Journal of Veterinary Internal Medicine"},{"key":"e_1_2_6_19_1","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.1111\/trf.14645","article-title":"False\u2010negative compatible antiglobulin crossmatches in samples with alloantibodies to cognate red blood cell antigens","volume":"58","author":"Nixon C.P.","year":"2018","journal-title":"Transfusion"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.2460\/ajvr.72.2.203","article-title":"Comparison of five blood\u2010typing methods for the feline AB blood group system","volume":"72","author":"Seth M.","year":"2011","journal-title":"American Journal of Veterinary Research"},{"key":"e_1_2_6_21_1","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1111\/jvim.15120","article-title":"Effect of cross\u2010match on packed cell volume after transfusion of packed red blood cells in transfusion\u2010na\u00efve anemic 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