{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:55:53Z","timestamp":1791276953453,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005667","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e Inova\u00e7\u00e3o do Estado de Santa Catarina","doi-asserted-by":"publisher","award":["25\/2025"],"award-info":[{"award-number":["25\/2025"]}],"id":[{"id":"10.13039\/501100005667","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Small Animal Practice"],"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To compare short\u2010term changes in the photographed percentage of visible tooth surface covered by pre\u2010existing dental calculus among dogs receiving additive\u2010free baked treats, sodium hexametaphosphate\u2010containing treats or pomegranate\u2013propolis\u2010containing treats, and to describe pooled supragingival bacterial profiles before and after the intervention. A fixed\u2010side brushing procedure was additionally explored from day 9 onward.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Twelve adult dogs (four per group) were enrolled in a 19\u2010day exploratory pilot study and randomly allocated to one of three treatment groups: additive\u2010free baked dental treats, baked dental treats containing 0.6% sodium hexametaphosphate or baked dental treats containing a 0.6% pomegranate\u2013propolis blend. Dogs received two treats daily throughout the study. A fixed\u2010side tooth\u2010brushing protocol was applied from day 9 onwards, with the right dental arch brushed daily and the left arch left unbrushed for within\u2010dog comparison. Photographed dental calculus coverage was assessed at predefined time points using standardised image analysis. Pooled supragingival bacterial profiles were descriptively characterised at baseline and day 19 by 16S rRNA gene sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Photographed dental calculus coverage declined in all treatment groups over the 19\u2010day study. The largest mean descriptive reduction was observed in dogs receiving baked dental treats containing the pomegranate\u2013propolis blend, followed by sodium hexametaphosphate and additive\u2010free baked dental treats. A greater descriptive reduction in photographed dental calculus coverage was observed on the brushed right dental arch after day 9. Exploratory analysis of pooled supragingival bacterial profiles showed descriptive differences in the relative abundance of bacterial taxa between baseline and day 19.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Significance<\/jats:title>\n                    <jats:p>In this 19\u2010day exploratory study, photographed dental calculus coverage declined in dogs receiving all three baked\u2010treat formulations. The largest mean descriptive reduction occurred in the pomegranate\u2013propolis group, followed by the sodium hexametaphosphate and additive\u2010free groups. Because the study included four dogs per treatment, no group without a baked treat, a fixed right\u2010side brushing protocol and pooled microbiome samples without biological replication, these findings should be interpreted as preliminary. Larger randomised studies beginning after standardised dental prophylaxis and using individual microbiome samples are required to determine preventive efficacy and clinical relevance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jsap.70207","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:47:30Z","timestamp":1791276450000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Short\u2010term changes in photographed dental calculus coverage and pooled supragingival microbiota after baked dental treats containing a pomegranate\u2013propolis blend or sodium hexametaphosphate in dogs"],"prefix":"10.1111","author":[{"given":"L. S. M.","family":"Baptista","sequence":"first","affiliation":[{"name":"Department of Animal Science Universidade Federal de Santa Catarina (UFSC)  Florian\u00f3polis Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. K. R.","family":"Santos","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Conventional and Integrative Veterinary Medicine (PPGMVCI) Universidade Federal de Santa Catarina (UFSC)  Florian\u00f3polis Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. A. T.","family":"Soratto","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biotechnology and Biosciences (PPGBIOTEC) Universidade Federal de Santa Catarina (UFSC)  Florian\u00f3polis Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biotechnology and Biosciences (PPGBIOTEC) Universidade Federal de Santa Catarina (UFSC)  Florian\u00f3polis Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Hauptli","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Conventional and Integrative Veterinary Medicine (PPGMVCI) Universidade Federal de Santa Catarina (UFSC)  Florian\u00f3polis Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Trevizan","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science (PPGZOT) Universidade Federal do Rio Grande do Sul (UFRGS)  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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