{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:46:45Z","timestamp":1791888405564,"version":"4.3.4"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Oral l-citrulline supplementation increases serum l-arginine levels more efficiently than l-arginine itself and increases nitric oxide (NO) production.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate whether oral l-citrulline supplementation improves erectile function in rats with acute arteriogenic erectile dysfunction (ED).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We divided 8-week-old male Wistar-ST rats into 3 groups: sham-operated rats (control group), arteriogenic ED rats who underwent ligation of both internal iliac arteries (ligation group), and arteriogenic ED rats receiving oral 2% l-citrulline water supplementation (citrulline group). Citrulline water was given to arteriogenic ED rats for 3 weeks from 1 week after surgery. Erectile function was evaluated by maximum intracavernous pressure\/mean arterial pressure (ICP\/MAP) ratios via cavernous nerve stimulation at 4 weeks after surgery. Then, the penises were resected, stained with Masson's trichrome, and observed microscopically. Serum nitrogen oxides (NOx) levels were measured by high-performance liquid chromatography. Bonferroni's multiple t-test was used for statistical analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main Outcome Measures<\/jats:title>\n                    <jats:p>The main outcome measures were changes in ICP\/MAP, smooth muscle (SM)\/collagen ratios, and NOx levels following l-citrulline supplementation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The ICP\/MAP ratio in the ligation group was significantly lower than that in the control group (P &amp;lt; 0.05), denoting ED. The ICP\/MAP ratio of the citrulline group was significantly higher than that of the ligation group (P &amp;lt; 0.05), indicating ED amelioration. Levels of NOx in the ligation group were significantly lower than in the control group (P &amp;lt; 0.05), while those in the citrulline group were significantly higher than in the ligation group (P &amp;lt; 0.05). SM\/collagen ratios in the ligation group were significantly lower than in the control group (P &amp;lt; 0.05), while ratios in the citrulline group were significantly higher than those in the ligation group (P &amp;lt; 0.05).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Oral l-citrulline supplementation improved ICP\/MAP and SM\/collagen ratios and increased NOx. Therefore, oral l-citrulline supplementation might be a useful novel therapy for acute arteriogenic ED.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jsm.12260","type":"journal-article","created":{"date-parts":[[2013,7,11]],"date-time":"2013-07-11T11:33:45Z","timestamp":1373542425000},"page":"2423-2429","source":"Crossref","is-referenced-by-count":33,"title":["Oral\n                    <scp>l<\/scp>\n                    -Citrulline Supplementation Improves Erectile Function in Rats with Acute Arteriogenic Erectile Dysfunction"],"prefix":"10.1093","volume":"10","author":[{"given":"Arufumi","family":"Shiota","sequence":"first","affiliation":[{"name":"Department of Hospital Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University , Nagoya, , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Hotta","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Graduate School of Pharmaceutical Sciences, 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