{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T05:17:31Z","timestamp":1771478251523,"version":"3.50.1"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Industrias C\u00e1rnicas Vaquero slaughterhouse","award":["PS09\/00044"],"award-info":[{"award-number":["PS09\/00044"]}]},{"name":"Industrias C\u00e1rnicas Vaquero slaughterhouse","award":["PR6\/13\u201318858"],"award-info":[{"award-number":["PR6\/13\u201318858"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Introduction<\/jats:title>\n                  <jats:p>Phosphodiesterase type 5 (PDE5) inhibitors act as effective drugs for the treatment of lower urinary tract symptom (LUTS). There is a poor information, however, about the role of the PDE4 inhibitors on the bladder outflow region contractility.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Aim<\/jats:title>\n                  <jats:p>To investigate PDE4 expression and the relaxation induced by the PDE4 inhibitor rolipram versus that induced by the PDE5 blockers sildenafil and vardenafil, in the pig and human bladder neck.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Immunohistochemistry for PDE4 expression, myographs for isometric force recordings and fura-2 fluorescence for simultaneous measurements of intracellular Ca2+ concentration ([Ca2+]i) and tension for rolipram in bladder neck samples were used.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Main Outcome Measures<\/jats:title>\n                  <jats:p>PDE4 expression and relaxations to PDE4 and PDE5 inhibitors and simultaneous measurements of [Ca2+]i and tension.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>PDE4 expression was observed widely distributed in the smooth muscle layer of the pig and human bladder neck. On urothelium-denuded phenylephrine (PhE)-precontracted strips of pig and human, rolipram, sildenafil and vardenafil produced concentration-dependent relaxations with the following order of potency: rolipram&amp;gt; &amp;gt; sildenafil&amp;gt;vardenafil. In pig, the adenylyl cyclase activator forskolin potentiated rolipram-elicited relaxation, whereas protein kinase A (PKA) blockade reduced such effect. On potassium-enriched physiological saline solution (KPSS)-precontracted strips, rolipram evoked a lower relaxation than that obtained on PhE-stimulated preparations. Inhibition of large (BKCa) and intermediate (IKCa) conductance Ca2+-activated K+ channels, neuronal voltage-gated Ca2+ channels, nitric oxide (NO) and hydrogen sulfide (H2S) synthases reduced rolipram responses. Rolipram inhibited the contractions induced by PhE without reducing the PhE-evoked [Ca2+]i increase.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>PDE4 is present in the pig and human bladder neck smooth muscle, where rolipram exerts a much more potent relaxation than that elicited by PDE5 inhibitors. In pig, rolipram-induced response is produced through the PKA pathway involving BKCa and IKCa channel activation and [Ca2+]idesensitization-dependent mechanisms, this relaxation also being due to neuronal NO and H2S release.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jsm.12456","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T12:36:26Z","timestamp":1392208586000},"page":"930-941","source":"Crossref","is-referenced-by-count":14,"title":["Powerful Relaxation of Phosphodiesterase Type 4 Inhibitor Rolipram in the Pig and Human Bladder Neck"],"prefix":"10.1093","volume":"11","author":[{"given":"Ana S.F.","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Departamento de Fisiolog\u00eda \u00a0 \u00a0 , Madrid , Spain"},{"name":"Facultad de Farmacia \u00a0 \u00a0 , Madrid , Spain"},{"name":"Universidad Complutense de Madrid \u00a0 \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edtor S.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda \u00a0 \u00a0 , Madrid , Spain"},{"name":"Facultad de Farmacia \u00a0 \u00a0 , Madrid , Spain"},{"name":"Universidad Complutense de Madrid \u00a0 \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Mart\u00ednez-S\u00e1enz","sequence":"additional","affiliation":[{"name":"Unidad Experimental \u00a0 \u00a0 , Madrid , Spain"},{"name":"Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica \u00a0 \u00a0 , Madrid , Spain"},{"name":"Hospital Puerta de Hierro-Majadahonda \u00a0 \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Departamento de Anatom\u00eda y Anatom\u00eda Patol\u00f3gica Comparadas \u00a0 \u00a0 , Madrid , Spain"},{"name":"Facultad de Veterinaria \u00a0 \u00a0 , Madrid , Spain"},{"name":"Universidad Complutense de Madrid \u00a0 \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Victoria","family":"Barahona","sequence":"additional","affiliation":[{"name":"Departamento de Toxicolog\u00eda y Farmacolog\u00eda \u00a0 \u00a0 , Madrid , Spain"},{"name":"Facultad de Veterinaria \u00a0 \u00a0 , Madrid , Spain"},{"name":"Universidad Complutense de Madrid \u00a0 \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis M.","family":"Orensanz","sequence":"additional","affiliation":[{"name":"Departamento de Investigaci\u00f3n \u00a0 , Madrid , Spain"},{"name":"Hospital Universitario Ram\u00f3n y Cajal \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor","family":"Blaha","sequence":"additional","affiliation":[{"name":"Departamento de Urolog\u00eda \u00a0 , Madrid , Spain"},{"name":"Hospital General Universitario Gregorio Mara\u00f1\u00f3n \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Serrano-Marg\u00fcello","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda \u00a0 \u00a0 , Madrid , Spain"},{"name":"Facultad de Farmacia \u00a0 \u00a0 , Madrid , Spain"},{"name":"Universidad Complutense de Madrid \u00a0 \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvador","family":"Bustamante","sequence":"additional","affiliation":[{"name":"Departamento de Urolog\u00eda \u00a0 , Madrid , Spain"},{"name":"Hospital Universitario Puerta de Hierro-Majadahonda \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaqu\u00edn","family":"Carballido","sequence":"additional","affiliation":[{"name":"Departamento de Urolog\u00eda \u00a0 , Madrid , Spain"},{"name":"Hospital Universitario Puerta de Hierro-Majadahonda \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albino","family":"Garc\u00eda-Sacrist\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda \u00a0 \u00a0 , Madrid , Spain"},{"name":"Facultad de Farmacia \u00a0 \u00a0 , Madrid , Spain"},{"name":"Universidad Complutense de Madrid \u00a0 \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dolores","family":"Prieto","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda \u00a0 \u00a0 , Madrid , Spain"},{"name":"Facultad de Farmacia \u00a0 \u00a0 , Madrid , Spain"},{"name":"Universidad Complutense de Madrid \u00a0 \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Medardo","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda \u00a0 \u00a0 , Madrid , Spain"},{"name":"Facultad de Farmacia \u00a0 \u00a0 , Madrid , Spain"},{"name":"Universidad Complutense de Madrid \u00a0 \u00a0 , Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,4,1]]},"reference":[{"key":"2024032602361703100_bib1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1540\/jsmr.39.67","article-title":"Phosphodiesterases in the vascular system","volume":"39","author":"Matsumoto","year":"2003","journal-title":"J Smooth Muscle Res"},{"key":"2024032602361703100_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1540\/jsmr.41.1","article-title":"Regulation of cyclic nucleotides in the urinary tract","volume":"41","author":"Wheeler","year":"2005","journal-title":"J Smooth Muscle Res"},{"key":"2024032602361703100_bib3","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.ejphar.2007.12.017","article-title":"PDE4 and PDE5 regulate cyclic nucleotides relaxing effects in human umbilical arteries","volume":"582","author":"Santos-Silva","year":"2008","journal-title":"Eur J Pharmacol"},{"key":"2024032602361703100_bib4","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.1111\/j.1743-6109.2010.01808.x","article-title":"Expression of cyclic AMP-dependent protein kinase isoforms in human cavernous arteries: functional significance and relation to phosphodiesterase type 4","volume":"7","author":"Waldkirch","year":"2010","journal-title":"J Sex Med"},{"key":"2024032602361703100_bib5","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/j.1476-5381.2009.00519.x","article-title":"Mechanisms involved in the regulation of bovine pulmonary vascular tone by the 5-HT1B receptor","volume":"159","author":"McKenzie","year":"2010","journal-title":"Br J Pharmacol"},{"key":"2024032602361703100_bib6","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1111\/jsm.12058","article-title":"Inhibition of phosphodiesterase 4 enhances clitoral and vaginal blood flow responses to dorsal clitoral nerve stimulation or PGE1 in anesthetized female rats","volume":"10","author":"Castiglione","year":"2013","journal-title":"J Sex Med"},{"key":"2024032602361703100_bib7","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.urology.2010.04.035","article-title":"Expression of cAMP-dependent protein kinase isoforms in the human prostate: functional significance and relation to PDE4","volume":"76","author":"Waldkirch","year":"2010","journal-title":"Urology"},{"key":"2024032602361703100_bib8","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.eururo.2006.09.026","article-title":"Effect of sildenafil and rolipram on adrenergic responses in isolated human and monkey corpus cavernosum","volume":"52","author":"Lau","year":"2007","journal-title":"Eur Urol"},{"key":"2024032602361703100_bib9","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.urology.2007.02.049","article-title":"Functional responses of isolated human seminal vesicle tissue to selective phosphodiesterase inhibitors","volume":"70","author":"Uckert","year":"2007","journal-title":"Urology"},{"key":"2024032602361703100_bib10","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1111\/j.1743-6109.2008.01085.x","article-title":"Effects of phosphodiesterase inhibitors on the contractile responses of isolated human seminal vesicle tissue to adrenergic stimulation","volume":"6","author":"Uckert","year":"2009","journal-title":"J Sex Med"},{"key":"2024032602361703100_bib11","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1007\/s00702-001-0684-1","article-title":"Effects of rolipram on in vivo dopamine receptor binding","volume":"109","author":"Hosoi","year":"2002","journal-title":"J Neural Transm"},{"key":"2024032602361703100_bib12","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.neuroscience.2006.09.026","article-title":"Rolipram: a specific phosphodiesterase 4 inhibitor with potential antipsychotic activity","volume":"144","author":"Kanes","year":"2007","journal-title":"Neuroscience"},{"key":"2024032602361703100_bib13","doi-asserted-by":"crossref","first-page":"C1255","DOI":"10.1152\/ajpcell.00381.2004","article-title":"Differential regulation of Ca2+-activated K+ channels by beta-adrenoceptors in guinea pig urinary bladder smooth muscle","volume":"288","author":"Petkov","year":"2005","journal-title":"Am J Physiol Cell Physiol"},{"key":"2024032602361703100_bib14","doi-asserted-by":"crossref","first-page":"F1149","DOI":"10.1152\/ajprenal.00440.2007","article-title":"Beta-adrenergic relaxation of mouse urinary bladder smooth muscle in the absence of large-conductance Ca2+-activated K+ channel","volume":"295","author":"Brown","year":"2008","journal-title":"Am J Physiol Renal Physiol"},{"key":"2024032602361703100_bib15","doi-asserted-by":"crossref","first-page":"C1344","DOI":"10.1152\/ajpcell.00001.2008","article-title":"Stimulation of beta3-adrenoceptors relaxes rat urinary bladder smooth muscle via activation of the large-conductance Ca2+-activated K+ channels","volume":"295","author":"Hristov","year":"2008","journal-title":"Am J Physiol Cell Physiol"},{"key":"2024032602361703100_bib16","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1016\/j.eururo.2006.10.023","article-title":"Relaxation of phasic contractile activity of human detrusor strips by cyclic nucleotide phosphodiesterase type 4 inhibition","volume":"51","author":"Oger","year":"2007","journal-title":"Eur Urol"},{"key":"2024032602361703100_bib17","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1152\/physrev.00038.2003","article-title":"Urinary bladder contraction and relaxation: physiology and pathophysiology","volume":"84","author":"Andersson","year":"2004","journal-title":"Physiol Rev"},{"key":"2024032602361703100_bib18","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1002\/nau.20485","article-title":"Phosphodiesterases (PDEs) and PDE inhibitors for treatment of LUTS","volume":"26","author":"Andersson","year":"2007","journal-title":"Neurourol Urodyn"},{"key":"2024032602361703100_bib19","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1002\/nau.20500","article-title":"LUTS treatment: future treatment options","volume":"26","author":"Andersson","year":"2007","journal-title":"Neurourol Urodyn"},{"key":"2024032602361703100_bib20","doi-asserted-by":"crossref","first-page":"S135","DOI":"10.1016\/j.juro.2012.11.025","article-title":"Urodynamic effects of once daily tadalafil in men with lower urinary tract symptoms secondary to clinical benign prostatic hyperplasia: a randomized, placebo controlled 12-week clinical trial","volume":"189","author":"Dmochowski","year":"2013","journal-title":"J Urol"},{"key":"2024032602361703100_bib21","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/iju.12008","article-title":"Latest treatment for lower urinary tract dysfunction: therapeutic agents and mechanism of action","volume":"20","author":"Yamaguchi","year":"2013","journal-title":"Int J Urol"},{"key":"2024032602361703100_bib22","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1038\/sj.bjp.0701328","article-title":"The role of cyclic nucleotides in guinea-pig bladder contractility","volume":"121","author":"Longhurst","year":"1997","journal-title":"Br J Pharmacol"},{"key":"2024032602361703100_bib23","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1111\/j.1464-410X.2006.06643.x","article-title":"Suppression of detrusor overactivity in rats with bladder outlet obstruction by a type 4 phosphodiesterase inhibitor","volume":"99","author":"Nishiguchi","year":"2007","journal-title":"BJU Int"},{"key":"2024032602361703100_bib24","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1111\/j.1464-410X.2007.07307.x","article-title":"The effects of a type 4 phosphodiesterase inhibitor and the muscarinic cholinergic antagonist tolterodine tartrate on detrusor overactivity in female rats with bladder outlet obstruction","volume":"101","author":"Kaiho","year":"2008","journal-title":"BJU Int"},{"key":"2024032602361703100_bib25","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.ejphar.2008.02.022","article-title":"Effect of phosphodiesterase type 4 inhibitor rolipram on cyclophosphamide-induced cystitis in rats","volume":"586","author":"B\u00fcy\u00fcknacar","year":"2008","journal-title":"Eur J Pharmacol"},{"key":"2024032602361703100_bib26","first-page":"764","article-title":"Significance of phosphodiesterase isoenzymes in the control of human detrusor smooth muscle function. An immunohistochemical and functional study","volume":"48","author":"Uckert","year":"2009","journal-title":"Urologe A"},{"key":"2024032602361703100_bib27","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1111\/j.1464-410X.2008.07948.x","article-title":"Rolipram, a specific type-4 phosphodiesterase inhibitor, inhibits cyclophosphamide-induced haemorrhagic cystitis in rats","volume":"103","author":"Sakura","year":"2009","journal-title":"BJU Int"},{"key":"2024032602361703100_bib28","doi-asserted-by":"crossref","first-page":"12107","DOI":"10.3390\/ijms140612107","article-title":"Eviprostat activates cAMP signaling pathway and suppresses bladder smooth muscle cell proliferation","volume":"14","author":"Li","year":"2013","journal-title":"Int J Mol Sci"},{"key":"2024032602361703100_bib29","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1038\/nrurol.2013.101","article-title":"The role of phosphodiesterases in bladder pathophysiology","volume":"10","author":"Rahnama'i","year":"2013","journal-title":"Nat Rev Urol"},{"key":"2024032602361703100_bib30","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1038\/sj.bjp.0707669","article-title":"Role of neuronal voltage-gated K(+) channels in the modulation of the nitrergic neurotransmission of the pig urinary bladder neck","volume":"153","author":"Hern\u00e1ndez","year":"2008","journal-title":"Br J Pharmacol"},{"key":"2024032602361703100_bib31","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1111\/j.1476-5381.2009.00314.x","article-title":"Role of ATP and related purines in inhibitory neurotransmission to the pig urinary bladder neck","volume":"157","author":"Hern\u00e1ndez","year":"2009","journal-title":"Br J Pharmacol"},{"key":"2024032602361703100_bib32","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1002\/nau.20287","article-title":"PACAP 38 is involved in the non adrenergic non cholinergic inhibitory neurotransmission in the pig urinary bladder neck","volume":"25","author":"Hern\u00e1ndez","year":"2006","journal-title":"Neurourol Urodyn"},{"key":"2024032602361703100_bib33","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1016\/j.juro.2012.10.006","article-title":"Endogenous hydrogen sulfide has a powerful role in inhibitory neurotransmission to the pig bladder neck","volume":"189","author":"Fernandes","year":"2013","journal-title":"J Urol"},{"key":"2024032602361703100_bib34","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.juro.2013.02.103","article-title":"Hydrogen sulfide-mediated inhibitory neurotransmission to the pig bladder neck: Role of KATP channels, sensory nerves and calcium signaling","volume":"190","author":"Fernandes","year":"2013","journal-title":"J Urol"},{"key":"2024032602361703100_bib35","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.steroids.2011.12.020","article-title":"Mechanisms involved in testosterone-induced relaxation to the pig urinary bladder neck","volume":"77","author":"Fernandes","year":"2012","journal-title":"Steroids"},{"key":"2024032602361703100_bib36","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1002\/nau.20999","article-title":"Tadalafil for the treatment of lower urinary tract symptoms secondary to benign prostatic hyperplasia: pathophysiology and mechanism(s) of action","volume":"30","author":"Andersson","year":"2011","journal-title":"Neurourol Urodyn"},{"key":"2024032602361703100_bib37","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1111\/jsm.12039","article-title":"Tadalafil once daily improves ejaculatory function, erectile function, and sexual satisfaction in men with lower urinary tract symptoms suggestive of benign prostatic hyperplasia and erectile dysfunction: results from a randomized, placebo- and tamsulosin-controlled, 12-week double-blind study","volume":"10","author":"Giuliano","year":"2013","journal-title":"J Sex Med"},{"key":"2024032602361703100_bib38","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.eururo.2012.09.006","article-title":"The mechanism of action of phosphodiesterase type 5 inhibitors in the treatment of lower urinary tract symptoms related to benign prostatic hyperplasia","volume":"63","author":"Giuliano","year":"2013","journal-title":"Eur Urol"},{"key":"2024032602361703100_bib39","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1111\/j.1743-6109.2012.02718.x","article-title":"A randomized, placebo-controlled study to assess safety and efficacy of vardenafil 10 mg and tamsulosin 0.4 mg versus tamsulosin 0.4 mg alone in the treatment of lower urinary tract symptoms secondary to benign prostatic hyperplasia","volume":"9","author":"Gacci","year":"2012","journal-title":"J Sex Med"},{"key":"2024032602361703100_bib40","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1111\/j.1464-410X.2007.07011.x","article-title":"The effects of exogenous prostaglandins and the identification of constitutive cyclooxygenase I and II immunoreactivity in the normal guinea pig bladder","volume":"100","author":"de Jongh","year":"2007","journal-title":"BJU Int"},{"key":"2024032602361703100_bib41","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/nau.20784","article-title":"Detrusor myocyte activity and afferent signaling","volume":"29","author":"Andersson","year":"2010","journal-title":"Neurourol Urodyn"},{"key":"2024032602361703100_bib42","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/sj.bjp.0706832","article-title":"Neuronal and smooth muscle receptors involved in the PACAP- and VIP-induced relaxations of the pig urinary bladder neck","volume":"149","author":"Hern\u00e1ndez","year":"2006","journal-title":"Br J Pharmacol"},{"key":"2024032602361703100_bib43","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/j.1476-5381.2009.00144.x","article-title":"5-hydroxytryptamine induced relaxation in the pig urinary bladder neck","volume":"157","author":"Recio","year":"2009","journal-title":"Br J Pharmacol"},{"key":"2024032602361703100_bib44","doi-asserted-by":"crossref","first-page":"R402","DOI":"10.1152\/ajpregu.00488.2004","article-title":"Negative feedback regulation of nerve-mediated contractions by KCa channels in mouse urinary bladder smooth muscle","volume":"289","author":"Herrera","year":"2005","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"2024032602361703100_bib45","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1002\/nau.22203","article-title":"Endothelin ET(B) receptors are involved in the relaxation to the pig urinary bladder neck","volume":"31","author":"Arteaga","year":"2012","journal-title":"Neurourol Urodyn"},{"key":"2024032602361703100_bib46","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1038\/sj.ijir.3900399","article-title":"Prostaglandin E1 activates the large-conductance KCa channel in human corporal smooth muscle cells","volume":"11","author":"Lee","year":"1999","journal-title":"Int J Impot Res"},{"key":"2024032602361703100_bib47","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1152\/physrev.00023.2003","article-title":"Ca2+ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases and myosin phosphatase","volume":"83","author":"Somlyo","year":"2003","journal-title":"Physiol Rev"},{"key":"2024032602361703100_bib48","doi-asserted-by":"crossref","first-page":"9912","DOI":"10.1016\/S0021-9258(17)36969-7","article-title":"Ca(2+)-dependent phosphorylation of myosin light chain kinase decreases the Ca2+ sensitivity of light chain phosphorylation within smooth muscle cells","volume":"269","author":"Tansey","year":"1994","journal-title":"J Biol Chem"},{"key":"2024032602361703100_bib49","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1111\/j.1743-6109.2012.02821.x","article-title":"Tadalafil enhances the inhibitory effects of tamsulosin on neurogenic contractions of human prostate and bladder neck","volume":"9","author":"Angulo","year":"2012","journal-title":"J Sex Med"},{"key":"2024032602361703100_bib50","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1016\/j.eururo.2012.07.025","article-title":"Sildenafil effect on the human bladder involves the L-cysteine\/hydrogen sulfide pathway: a novel mechanism of action of phosphodiesterase type 5 inhibitors","volume":"62","author":"Fusco","year":"2012","journal-title":"Eur Urol"},{"key":"2024032602361703100_bib51","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1016\/j.urology.2008.11.041","article-title":"Effects of phosphodiesterase inhibitors on contraction induced by endothelin-1 of isolated human prostatic tissue","volume":"73","author":"Kedia","year":"2009","journal-title":"Urology"},{"key":"2024032602361703100_bib52","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1016\/S0022-5347(01)62833-7","article-title":"The effect of the specific phosphodiesterase-IV-inhibitor rolipram on the ureteral peristalsis of the rabbit in vitro and in vivo","volume":"160","author":"Becker","year":"1998","journal-title":"J Urol"}],"container-title":["The Journal of Sexual Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1743609515307414?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1743609515307414?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jsm\/article-pdf\/11\/4\/930\/48359446\/jsm_11_4_930.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jsm\/article-pdf\/11\/4\/930\/48359446\/jsm_11_4_930.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T03:09:16Z","timestamp":1711422556000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jsm\/article\/11\/4\/930\/6958258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,1]]},"references-count":52,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2014,4,1]]},"published-print":{"date-parts":[[2014,4,1]]}},"URL":"https:\/\/doi.org\/10.1111\/jsm.12456","relation":{},"ISSN":["1743-6109","1743-6095"],"issn-type":[{"value":"1743-6109","type":"electronic"},{"value":"1743-6095","type":"print"}],"subject":[],"published-other":{"date-parts":[[2014,4]]},"published":{"date-parts":[[2014,4,1]]}}}