{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:24:30Z","timestamp":1792787070802,"version":"4.5.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81070486\/H0415"],"award-info":[{"award-number":["81070486\/H0415"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Outstanding Youth Foundation of Sichuan Province","award":["2010JQ0040"],"award-info":[{"award-number":["2010JQ0040"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The impaired erectile response in spontaneously hypertensive rats (SHR) is caused by increased signaling of RhoA\/Rho-kinase and decreased signaling of nitric oxide (NO). Icariin improves erectile function via upregulating multitargets in NO\/cyclic guanosine monophosphate (NO\/cGMP) pathway, which breviscapine accomplishes by downregulating RhoA\/Rho-kinase pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the effect and mechanism of icariin combined with breviscapine on the erectile function of SHR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Five 12-week-old male Wistar-Kyoto (WKY) rats and 20 age-matched male SHR were evenly randomized into WKY rats control group, SHR control group, icariin-treated group, breviscapine-treated group, and combined treatment group treated by vehicle, icariin, breviscapine, and icariin plus breviscapine, respectively, by gavage for four successive weeks. Maximum intracavernosal pressure\/mean arterial pressure (ICPmax\/MAP) and the expression of endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase (nNOS), phosphodiesterase type 5 inhibitors (PDE5), and Rho-associated, coiled-coil containing protein kinase 1 and 2 (ROCK1 and ROCK2) in the cavernous tissues were determined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The ICPmax\/MAP in the combined treatment group was significantly increased compared with SHR control group, icariin-treated group, and breviscapine-treated group. The expression of eNOS and nNOS was significantly higher in the combined treatment group than in SHR control group, icariin-treated group, and breviscapine-treated group (P &amp;lt; 0.05). The expression of PDE5 was significantly lower in the icariin-treated group than in SHR control group (P &amp;lt; 0.05). The expression of ROCK1 was significantly lower in the combined treatment group than in other groups (P &amp;lt; 0.05). The expression of ROCK2 was significantly higher in SHR control group than in WKY rats control group, icariin-treated group, and combined treatment group (P &amp;lt; 0.05). Among these groups, the expression of eNOS and nNOS was the strongest, and ROCK1 was the lowest in WKY rats control group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Icariin combined with breviscapine has synergistic effects on erectile function of SHR through different signal pathways.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jsm.12614","type":"journal-article","created":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T06:25:20Z","timestamp":1402381520000},"page":"2143-2152","source":"Crossref","is-referenced-by-count":17,"title":["Icariin Combined with Breviscapine Improves the Erectile Function of Spontaneously Hypertensive Rats"],"prefix":"10.1093","volume":"11","author":[{"given":"Yongxian","family":"Li","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Diseases \u00a0 , Luzhou , China"},{"name":"Affiliated Hospital, Luzhou 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