{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:20:37Z","timestamp":1773706837303,"version":"3.50.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Resveratrol (RVT) found in red wine protects against erectile dysfunction and relaxes penile tissue (corpus cavernosum) via a nitric oxide (NO) independent pathway. However, the mechanism remains to be elucidated. Hydrogen sulfide (H2S) is a potent vasodilator and neuromodulator generated in corpus cavernosum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>We investigated whether RVT caused the relaxation of mice corpus cavernosum (MCC) through H2S.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>H2S formation is measured by methylene blue assay and vascular reactivity experiments have been performed by DMT strip myograph in CD1 MCC strips.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main Outcome Measures<\/jats:title><jats:p>Endothelial NO synthase (eNOS) inhibitor N\u03c9-Nitro-L-arginine (L-NNA, 0.1\u2009mM) or H2S inhibitor aminooxyacetic acid (AOAA, 2\u2009mM) which inhibits both cystathionine-\u03b2-synthase (CBS) and cystathionine-gamma-lyase (CSE) enzyme or combination of AOAA with PAG (CSE inhibitor) has been used in the presence\/absence of RVT (0.1\u2009mM, 30\u2009min) to elucidate the role of NO or H2S pathways on the effects of RVT in MCC. Concentration-dependent relaxations to RVT, L-cysteine, sodium hydrogen sulfide (NaHS) and acetylcholine (ACh) were studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Exposure of murine corpus cavernosum to RVT increased both basal and L-cysteine-stimulated H2S formation. Both of these effects were reversed by AOAA but not by L-NNA. RVT caused concentration-dependent relaxation of MCC and that RVT-induced relaxation was significantly inhibited by AOAA or AOAA+PAG but not by L-NNA. L-cysteine caused concentration-dependent relaxations, which are inhibited by AOAA or AOAA+PAG significantly. Incubation of MCC with RVT significantly increased L-cysteine-induced relaxation, and this effect was inhibited by AOAA+PAG. However, RVT did not alter the effect of exogenous H2S (NaHS) or ACh-induced relaxations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results demonstrate that RVT-induced relaxation is at least partly dependent on H2S formation and acts independent of eNOS pathway. In phosphodiesterase 5 inhibitor (PDE-5i) nonresponder population, combination therapy with RVT may reverse erectile dysfunction via stimulating endogenous H2S formation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jsm.12993","type":"journal-article","created":{"date-parts":[[2015,10,6]],"date-time":"2015-10-06T06:36:46Z","timestamp":1444113406000},"page":"2004-2012","source":"Crossref","is-referenced-by-count":23,"title":["Resveratrol Stimulates Hydrogen Sulfide (H2S) Formation to Relax Murine Corpus Cavernosum"],"prefix":"10.1093","volume":"12","author":[{"given":"G.","family":"Yetik-Anacak","sequence":"first","affiliation":[{"name":"Department of Pharmacology \u00a0 \u00a0 , Izmir , Turkey"},{"name":"Faculty of Pharmacy \u00a0 \u00a0 , Izmir , Turkey"},{"name":"Ege University \u00a0 \u00a0 , Izmir , Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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