{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:33:27Z","timestamp":1791891207370,"version":"4.3.4"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["CB\u2010255936\/2015"],"award-info":[{"award-number":["CB\u2010255936\/2015"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Sleep Research"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sleep loss increases blood\u2013brain barrier permeability. As the blood\u2013brain barrier and the blood\u2013tissue barriers in the reproductive tract (blood\u2013testis and blood\u2013epididymis barriers) share common characteristics, we hypothesized that sleep restriction may also modify their barrier function. Previous reports showed that sleep loss decreased sperm viability and progressive fast mobility, which may be a consequence of altered blood\u2013testis and blood\u2013epididymis barrier. Therefore, we quantified changes in blood\u2013testis and blood\u2013epididymis barrier after sleep loss and related them to male fertility. Adult male Wistar rats were sleep restricted using the multiple\u2010platform technique in a protocol of 20\u00a0hr daily sleep deprivation plus 4\u00a0hr of sleep recovery in the home\u2010cage. At the 10th day, barrier permeability assays were performed with Na\u2010fluorescein, 10\u00a0kDa Cascade blue\u2010dextrans and Evans blue, and the expression of tight junction proteins, actin and androgen receptor was quantified. At the 10th day of sleep restriction and after sleep recovery days 1\u20137, males were placed with sexually receptive females, sexual behaviour was tested, and the percentage of pregnancies was calculated. Sleep restriction increased the barrier permeability to low\u2010 and high\u2010molecular\u2010weight tracers, and decreased the expression of tight junction proteins, actin and androgen receptor. Concomitantly, sleep restriction reduced the percentage of ejaculating males and the number of pregnancies. Sleep recovery for 2\u20133\u00a0days progressively re\u2010established fertility, as indicated by a higher percentage of ejaculating males and impregnated females. In conclusion, chronic sleep loss alters fertility concomitantly with the disruption of the blood\u2013tissue barriers at the reproductive tract, the mechanism involves androgen signalling.<\/jats:p>","DOI":"10.1111\/jsr.12907","type":"journal-article","created":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T05:11:46Z","timestamp":1570079506000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Chronic sleep loss disrupts blood\u2013testis and blood\u2013epididymis barriers, and reduces male fertility"],"prefix":"10.1111","volume":"29","author":[{"given":"Emilio","family":"Dom\u00ednguez\u2010Salazar","sequence":"first","affiliation":[{"name":"Department of Biology of Reproduction Area of Neurosciences CBS, Universidad Aut\u00f3noma Metropolitana, Unidad Iztapalapa Mexico City 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