{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T04:07:01Z","timestamp":1788408421101,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T00:00:00Z","timestamp":1631404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Sleep Research"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Insomnia is one of the most prevalent sleep disorders, which imparts tremendous societal and economic impact. However, the present pharmacotherapy is greatly limited by adverse effects, so it is necessary to explore new drugs for the treatment of insomnia. <jats:italic>Radix Bupleuri<\/jats:italic> (RB) has been widely used in traditional Chinese medicine for &gt;2000\u00a0years; it has many pharmacological effects, including sedation and anticonvulsant properties. The present study investigated the effects of saikosaponin a (SSa), an active component of RB, on sleep and locomotion. Male C57BL\/6j mice received intraperitoneal injections of SSa at three different dosages (0.625, 1.25, and 2.5\u00a0mg\/kg). Sleep parameters were analysed by electroencephalography and electromyography. The open\u2010field test was used to measure locomotor activities. Our present results showed that SSa treatment significantly increased the duration of non\u2010rapid eye movement sleep and shortened sleep latency in a dose\u2010dependent manner. A high dose of SSa (2.5\u00a0mg\/kg) also decreased locomotor activities. Moreover, by measuring c\u2010Fos expression and the calcium signal in the lateral hypothalamus (LH), we found that SSa treatment decreased neuronal activity in the LH. In conclusion, SSa might be the sleep\u2010promoting component in RB and its mechanism may be related to the modulation of neuronal activity in the LH.<\/jats:p>","DOI":"10.1111\/jsr.13484","type":"journal-article","created":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T17:28:00Z","timestamp":1631467680000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Saikosaponin a promotes sleep by decreasing neuronal activities in the lateral hypothalamus"],"prefix":"10.1111","volume":"31","author":[{"given":"Yu\u2010Heng","family":"Zhong","sequence":"first","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Min","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine Zhongshan Hospital Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9359-1150","authenticated-orcid":false,"given":"Zhi\u2010Li","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9879-9620","authenticated-orcid":false,"given":"Chang\u2010Rui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06310"},{"key":"e_1_2_9_3_1","first-page":"22","article-title":"Sleep disorders: Insomnia","volume":"460","author":"Burman D.","year":"2017","journal-title":"FP Essent"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2013.193"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(00)81973\u2010x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.5694\/mja13.10718"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.22495"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311\u2010019\u201000827\u2010z"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.00242"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4377"},{"key":"e_1_2_9_11_1","volume-title":"The mouse brain in stereotaxic coordinates: Compact","author":"Franklin K. 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