{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:58:26Z","timestamp":1777377506700,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T00:00:00Z","timestamp":1721433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005969","name":"Universit\u00e0 di Bologna","doi-asserted-by":"publisher","award":["RFO 2020\u20102022"],"award-info":[{"award-number":["RFO 2020\u20102022"]}],"id":[{"id":"10.13039\/501100005969","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Sleep Research"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Narcolepsy type\u20101 (NT1) is a lifelong sleep disease, characterised by impairment of the orexinergic system, with a typical onset during adolescence and young adulthood. Since the wake\u2013sleep cycle physiologically changes with ageing, this study aims to compare sleep patterns between orexin\u2010knockout (KO) and wild type (WT) control mice at different ages. Four groups of age\u2010matched female KO and WT mice (16\u2009weeks of age: 8 KO\u2010YO and 9 WT\u2010YO mice; 87\u2009weeks of age: 13 KO\u2010OLD and 12 WT\u2010OLD mice) were implanted with electrodes for discriminating wakefulness, rapid\u2010eye\u2010movement sleep (REMS), and non\u2010REMS (NREMS). Mice were recorded for 48\u2009h in their home cages and for 7 more hours into a plethysmographic chamber to characterise their sleep\u2010breathing pattern. Regardless of orexin deficiency, OLD mice spent less time awake and had fragmentation of this behavioural state showing more bouts of shorter length than YO mice. OLD mice also had more NREMS bouts and less frequent NREMS apneas than YO mice. Regardless of age, KO mice showed cataplexy\u2010like episodes and shorter REMS latency than WT controls and had a faster breathing rate and an increased minute ventilation during REMS. KO mice also had more wakefulness, NREMS and REMS bouts, and a shorter mean length of wakefulness bouts than WT controls. Our experiment indicated that the lack of orexins as well as ageing importantly modulate the sleep and breathing phenotype in mice. The narcoleptic phenotype caused by orexin deficiency in female mice was substantially preserved with ageing.<\/jats:p>","DOI":"10.1111\/jsr.14287","type":"journal-article","created":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T09:53:56Z","timestamp":1721469236000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Ageing\u2010related modification of sleep and breathing in orexin\u2010knockout narcoleptic mice"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2468-1704","authenticated-orcid":false,"given":"Stefano","family":"Bastianini","sequence":"first","affiliation":[{"name":"Laboratory of Physiological Regulation in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences Alma Mater Studiorum \u2013 University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Alvente","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Regulation in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences Alma Mater Studiorum \u2013 University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Berteotti","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Regulation in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences Alma Mater Studiorum \u2013 University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviana","family":"Lo Martire","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Regulation in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences Alma Mater Studiorum \u2013 University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Matteoli","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Regulation in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences Alma Mater Studiorum \u2013 University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Miglioranza","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Regulation in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences Alma Mater Studiorum \u2013 University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3992-3892","authenticated-orcid":false,"given":"Alessandro","family":"Silvani","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Regulation in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences Alma Mater Studiorum \u2013 University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9670-9959","authenticated-orcid":false,"given":"Giovanna","family":"Zoccoli","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Regulation in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences Alma Mater Studiorum \u2013 University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/sleep\/zsac089"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsr.12845"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41698"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2014.07.018"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-03468-5"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/sleep\/34.2.213"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140520"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsr.13255"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.219485"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81973-X"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.279.1.R1"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2301940"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2012-1340"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012369454-6\/50074-1"},{"key":"e_1_2_10_16_1","first-page":"1","volume-title":"Normative biology, husbandry and models","author":"Fox J. 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