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Current assessments rely heavily on subjective measures, necessitating objective biomarkers. We investigated whether baseline electroencephalography (EEG) and sleep\u2013wake profiles can predict anxiety levels in a mouse model. Fifty male C57BL\/6N mice were implanted with EEG and electromyography (EMG) electrodes, recorded for 24\u2009h post\u2010recovery before undergoing a cued fear\u2010conditioning protocol. Six behavioural indices, capturing freezing during tone and intertone epochs in both conditioning and retrieval, were used in k\u2010medoids clustering to classify mice into high\u2010anxiety or low\u2010anxiety groups. Comparative analysis revealed pronounced group differences in baseline sleep architecture. HA mice exhibited diminished rapid eye movement sleep (REMS) proportions and elevated NREMS in the dark period, alongside altered bout lengths. Spectral analysis underscored lower delta (0.5\u20132\u2009Hz) power in high\u2010anxiety mice, with heightened eta\/beta (~15\u201330\u2009Hz) activity during REMS suggesting aberrant cortical arousal. Moreover, high\u2010anxiety mice showed significantly longer and higher\u2010amplitude sleep spindles, reinforcing the interplay between disrupted sleep microstructure and enhanced anxiety\u2010like responses. These findings illustrate that specific EEG and sleep\u2013wake parameters prior to a conditioned stimulus can forecast distinct anxiety phenotypes in a widely used inbred mouse model. Although our study focuses on fundamental mechanisms in laboratory animals, this line of research may lead to objective markers of anxious behaviour susceptibility in broader contexts, potentially identifying individuals at higher risk for heightened anxiety in clinically relevant settings, for example, preoperative anxiety.<\/jats:p>","DOI":"10.1111\/jsr.70425","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:12:50Z","timestamp":1785413570000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Distinct\n                    <scp>EEG<\/scp>\n                    Features and Sleep\/Wake Behaviour Predict Anxiety Phenotypes in Mice"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1861-8384","authenticated-orcid":false,"given":"A.","family":"Altunkaya","sequence":"first","affiliation":[{"name":"Clinic for Anesthesiology and Intensive Care, School of Medicine and Health Technical University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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