{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:53:29Z","timestamp":1791255209117,"version":"4.1.0"},"reference-count":54,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T00:00:00Z","timestamp":1670371200000},"content-version":"vor","delay-in-days":2866,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"NIH","award":["HL122401"],"award-info":[{"award-number":["HL122401"]}]}],"content-domain":{"domain":["jthjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Thrombosis and Haemostasis"],"published-print":{"date-parts":[[2015,2]]},"DOI":"10.1111\/jth.12795","type":"journal-article","created":{"date-parts":[[2014,11,22]],"date-time":"2014-11-22T01:23:38Z","timestamp":1416619418000},"page":"303-313","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":71,"title":["A novel conditional platelet depletion mouse model reveals the importance of platelets in protection against Staphylococcus aureus bacteremia"],"prefix":"10.1016","volume":"13","author":[{"given":"L.M.","family":"Wuescher","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Takashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.G.","family":"Worth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1111\/jth.12795_bb0010","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.thromres.2011.11.036","article-title":"Platelets and primary haemostasis","volume":"129","author":"Clemetson","year":"2012","journal-title":"Thromb Res"},{"key":"10.1111\/jth.12795_bb0015","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1038\/nri2956","article-title":"Platelets and the immune continuum","volume":"11","author":"Semple","year":"2011","journal-title":"Nat Rev Immunol"},{"key":"10.1111\/jth.12795_bb0020","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1182\/blood-2005-06-2202","article-title":"Platelet Toll\u2010like receptor expression modulates lipopolysaccharide\u2010induced thrombocytopenia and tumor necrosis factor\u2010alpha production in\u00a0vivo","volume":"107","author":"Aslam","year":"2006","journal-title":"Blood"},{"key":"10.1111\/jth.12795_bb0025","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1111\/j.1440-1711.2005.01314.x","article-title":"Evidence of Toll\u2010like receptor molecules on human platelets","volume":"83","author":"Cognasse","year":"2005","journal-title":"Immunol Cell Biol"},{"key":"10.1111\/jth.12795_bb0030","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1172\/JCI562","article-title":"Platelet microbicidal proteins and neutrophil defensin disrupt the Staphylococcus aureus cytoplasmic membrane by distinct mechanisms of action","volume":"101","author":"Yeaman","year":"1998","journal-title":"J Clin Invest"},{"key":"10.1111\/jth.12795_bb0035","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1128\/iai.65.3.1023-1031.1997","article-title":"Purification and in\u00a0vitro activities of rabbit platelet microbicidal proteins","volume":"65","author":"Yeaman","year":"1997","journal-title":"Infect Immun"},{"key":"10.1111\/jth.12795_bb0040","doi-asserted-by":"crossref","first-page":"623","DOI":"10.4049\/jimmunol.167.2.623","article-title":"Cutting edge: IFN\u2010inducible ELR\u2010CXC chemokines display defensin\u2010like antimicrobial activity","volume":"167","author":"Cole","year":"2001","journal-title":"J Immunol"},{"key":"10.1111\/jth.12795_bb0045","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1111\/j.1538-7836.2009.03586.x","article-title":"Platelet functions beyond hemostasis","volume":"7","author":"Smyth","year":"2009","journal-title":"J Thromb Haemost"},{"key":"10.1111\/jth.12795_bb0050","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1126\/science.1181928","article-title":"Platelets amplify inflammation in arthritis via collagen\u2010dependent microparticle production","volume":"327","author":"Boilard","year":"2010","journal-title":"Science"},{"key":"10.1111\/jth.12795_bb0055","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1038\/nm1317","article-title":"Platelets mediate cytotoxic T\u00a0lymphocyte\u2010induced liver damage","volume":"11","author":"Iannacone","year":"2005","journal-title":"Nat Med"},{"key":"10.1111\/jth.12795_bb0060","doi-asserted-by":"crossref","first-page":"4021","DOI":"10.1182\/blood-2001-12-0191","article-title":"Host defense role of platelets: engulfment of HIV and Staphylococcus aureus occurs in a specific subcellular compartment and is enhanced by platelet activation","volume":"99","author":"Youssefian","year":"2002","journal-title":"Blood"},{"key":"10.1111\/jth.12795_bb0065","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1128\/CVI.00296-10","article-title":"Internalization of IgG\u2010coated targets results in activation and secretion of soluble CD40 ligand and RANTES by human platelets","volume":"18","author":"Antczak","year":"2011","journal-title":"Clin Vaccine Immunol"},{"key":"10.1111\/jth.12795_bb0070","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.clim.2012.09.004","article-title":"Human platelets can discriminate between various bacterial LPS isoforms via TLR4 signaling and differential cytokine secretion","volume":"145","author":"Berthet","year":"2012","journal-title":"Clin Immunol"},{"key":"10.1111\/jth.12795_bb0075","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1111\/j.1574-695X.2012.00945.x","article-title":"Human platelets efficiently kill IgG\u2010opsonized E.\u00a0coli","volume":"65","author":"Riaz","year":"2012","journal-title":"FEMS Immunol Med Microbiol"},{"key":"10.1111\/jth.12795_bb0080","doi-asserted-by":"crossref","first-page":"384685","DOI":"10.1155\/2012\/384685","article-title":"Crosstalk between platelets and the immune system: old systems with new discoveries","volume":"2012","author":"Li","year":"2012","journal-title":"Adv Hematol"},{"key":"10.1111\/jth.12795_bb0085","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1084\/jem.20112322","article-title":"Monocytes, neutrophils, and platelets cooperate to initiate and propagate venous thrombosis in mice in\u00a0vivo","volume":"209","author":"von Bruhl","year":"2012","journal-title":"J Exp Med"},{"key":"10.1111\/jth.12795_bb0090","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.thromres.2012.11.028","article-title":"Platelet\u2013leukocyte crosstalk: linking proinflammatory responses to procoagulant state","volume":"131","author":"Ghasemzadeh","year":"2013","journal-title":"Thromb Res"},{"key":"10.1111\/jth.12795_bb0095","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.chom.2012.06.011","article-title":"Intravascular neutrophil extracellular traps capture bacteria from the bloodstream during sepsis","volume":"12","author":"McDonald","year":"2012","journal-title":"Cell Host Microbe"},{"key":"10.1111\/jth.12795_bb0100","doi-asserted-by":"crossref","first-page":"e1002355","DOI":"10.1371\/journal.ppat.1002355","article-title":"Novel anti\u2010bacterial activities of beta\u2010defensin\u00a01 in human platelets: suppression of pathogen growth and signaling of neutrophil extracellular trap formation","volume":"7","author":"Kraemer","year":"2011","journal-title":"PLoS Pathog"},{"key":"10.1111\/jth.12795_bb0105","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/ni.2631","article-title":"Nucleation of platelets with blood\u2010borne pathogens on Kupffer cells precedes other innate immunity and contributes to bacterial clearance","volume":"14","author":"Wong","year":"2013","journal-title":"Nat Immunol"},{"key":"10.1111\/jth.12795_bb0110","doi-asserted-by":"crossref","first-page":"2657","DOI":"10.1038\/ncomms3657","article-title":"Platelets protect from septic shock by inhibiting macrophage\u2010dependent inflammation via the cyclooxygenase\u00a01 signalling pathway","volume":"4","author":"Xiang","year":"2013","journal-title":"Nat Commun"},{"key":"10.1111\/jth.12795_bb0115","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1056\/NEJM199808203390806","article-title":"Staphylococcus aureus infections","volume":"339","author":"Lowy","year":"1998","journal-title":"N Engl J Med"},{"key":"10.1111\/jth.12795_bb0120","doi-asserted-by":"crossref","first-page":"S350","DOI":"10.1086\/533591","article-title":"Pathogenesis of methicillin\u2010resistant Staphylococcus aureus infection","volume":"46","author":"Gordon","year":"2008","journal-title":"Clin Infect Dis"},{"key":"10.1111\/jth.12795_bb0125","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0891-5520(01)00009-5","article-title":"Pathogenesis of streptococcal and staphylococcal endocarditis","volume":"16","author":"Moreillon","year":"2002","journal-title":"Infect Dis Clin North Am"},{"key":"10.1111\/jth.12795_bb0130","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1093\/infdis\/jit235","article-title":"Staphylococcus aureus alpha\u2010hemolysin promotes platelet\u2013neutrophil aggregate formation","volume":"208","author":"Parimon","year":"2013","journal-title":"J Infect Dis"},{"key":"10.1111\/jth.12795_bb0135","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/0092-8674(95)90531-6","article-title":"Transcription factor NF\u2010E2 is required for platelet formation independent of the actions of thrombopoietin\/MGDF in megakaryocyte development","volume":"81","author":"Shivdasani","year":"1995","journal-title":"Cell"},{"key":"10.1111\/jth.12795_bb0140","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1038\/nmeth762","article-title":"A Cre\u2010inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration","volume":"2","author":"Buch","year":"2005","journal-title":"Nat Methods"},{"issue":"Suppl. 1","key":"10.1111\/jth.12795_bb0145","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/j.thromres.2014.03.002","article-title":"Adoptive transfer method to study platelet function in mouse models of disease","volume":"133","author":"Bergmeier","year":"2014","journal-title":"Thromb Res"},{"key":"10.1111\/jth.12795_bb0150","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1182\/blood-2006-04-020362","article-title":"Pf4\u2010Cre transgenic mice allow the generation of lineage\u2010restricted gene knockouts for studying megakaryocyte and platelet function in\u00a0vivo","volume":"109","author":"Tiedt","year":"2007","journal-title":"Blood"},{"key":"10.1111\/jth.12795_bb0155","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1016\/j.exphem.2006.06.015","article-title":"Platelet FcgammaRIIA binds and internalizes IgG\u2010containing complexes","volume":"34","author":"Worth","year":"2006","journal-title":"Exp Hematol"},{"key":"10.1111\/jth.12795_bb0160","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.cellimm.2010.03.009","article-title":"IgG\u2010complex stimulated platelets: a source of sCD40L and RANTES in initiation of inflammatory cascade","volume":"263","author":"Antczak","year":"2010","journal-title":"Cell Immunol"},{"key":"10.1111\/jth.12795_bb0165","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25\u00a0years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat Methods"},{"key":"10.1111\/jth.12795_bb0170","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.ajpath.2012.09.005","article-title":"FcgammaRIIa ligation induces platelet hypersensitivity to thrombotic stimuli","volume":"182","author":"Berlacher","year":"2013","journal-title":"Am J Pathol"},{"key":"10.1111\/jth.12795_bb0175","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/1471-2431-11-96","article-title":"Higher incidence of perineal community acquired MRSA infections among toddlers","volume":"11","author":"McCullough","year":"2011","journal-title":"BMC Pediatr"},{"key":"10.1111\/jth.12795_bb0180","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1038\/90795","article-title":"Diphtheria toxin receptor\u2010mediated conditional and targeted cell ablation in transgenic mice","volume":"19","author":"Saito","year":"2001","journal-title":"Nat Biotechnol"},{"key":"10.1111\/jth.12795_bb0185","doi-asserted-by":"crossref","first-page":"4446","DOI":"10.1182\/blood.V92.11.4446","article-title":"P\u2010selectin and platelet clearance","volume":"92","author":"Berger","year":"1998","journal-title":"Blood"},{"key":"10.1111\/jth.12795_bb0190","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1083\/jcb.201304054","article-title":"The incredible journey: from megakaryocyte development to platelet formation","volume":"201","author":"Machlus","year":"2013","journal-title":"J Cell Biol"},{"key":"10.1111\/jth.12795_bb0195","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1182\/blood-2012-09-453894","article-title":"Genetic elimination of the binding motif on fibrinogen for the S.\u00a0aureus virulence factor ClfA improves host survival in septicemia","volume":"121","author":"Flick","year":"2013","journal-title":"Blood"},{"key":"10.1111\/jth.12795_bb0200","doi-asserted-by":"crossref","first-page":"e1002307","DOI":"10.1371\/journal.ppat.1002307","article-title":"Preventing Staphylococcus aureus sepsis through the inhibition of its agglutination in blood","volume":"7","author":"McAdow","year":"2011","journal-title":"PLoS Pathog"},{"key":"10.1111\/jth.12795_bb0205","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1007\/s00134-009-1723-x","article-title":"Histopathology of septic shock induced acute kidney injury: apoptosis and leukocytic infiltration","volume":"36","author":"Lerolle","year":"2010","journal-title":"Intensive Care Med"},{"key":"10.1111\/jth.12795_bb0210","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1097\/01.ASN.0000055652.37763.F7","article-title":"Prevention and treatment of acute renal failure in sepsis","volume":"14","author":"De Vriese","year":"2003","journal-title":"J Am Soc Nephrol"},{"key":"10.1111\/jth.12795_bb0215","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1128\/MMBR.05015-11","article-title":"Into the eye of the cytokine storm","volume":"76","author":"Tisoncik","year":"2012","journal-title":"Microbiol Mol Biol Rev"},{"key":"10.1111\/jth.12795_bb0220","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1093\/infdis\/175.1.118","article-title":"Antiinflammatory cytokine responses during clinical sepsis and experimental endotoxemia: sequential measurements of plasma soluble interleukin (IL)\u20101 receptor type\u00a0II, IL\u201010, and IL\u201013","volume":"175","author":"van der Poll","year":"1997","journal-title":"J Infect Dis"},{"key":"10.1111\/jth.12795_bb0225","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1007\/s00011-003-1239-3","article-title":"Are IL\u20106, IL\u201010 and PCT plasma concentrations reliable for outcome prediction in severe sepsis? A comparison with APACHE\u00a0III and SAPS\u00a0II","volume":"53","author":"Wunder","year":"2004","journal-title":"Inflamm Res"},{"key":"10.1111\/jth.12795_bb0230","doi-asserted-by":"crossref","first-page":"e59107","DOI":"10.1371\/journal.pone.0059107","article-title":"Distinctive cytokines as biomarkers predicting fatal outcome of severe Staphylococcus aureus bacteremia in mice","volume":"8","author":"van den Berg","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1111\/jth.12795_bb0235","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1007\/s00018-009-0210-4","article-title":"Platelets in defense against bacterial pathogens","volume":"67","author":"Yeaman","year":"2010","journal-title":"Cell Mol Life Sci"},{"key":"10.1111\/jth.12795_bb0240","first-page":"908","article-title":"Platelet ITAM signaling is critical for vascular integrity in inflammation","volume":"123","author":"Boulaftali","year":"2013","journal-title":"J Clin Investig"},{"issue":"25","key":"10.1111\/jth.12795_bb0245","doi-asserted-by":"crossref","first-page":"3781","DOI":"10.1182\/blood-2014-05-573915","article-title":"Thrombocytopenia impairs host defense in gram\u2010negative pneumonia derived sepsis","volume":"124","author":"de Stoppelaar","year":"2014","journal-title":"Blood"},{"key":"10.1111\/jth.12795_bb0250","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1126\/science.8073287","article-title":"Thrombocytopenia in c\u2010mpl\u2010deficient mice","volume":"265","author":"Gurney","year":"1994","journal-title":"Science"},{"key":"10.1111\/jth.12795_bb0255","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1002\/stem.5530140716","article-title":"Studies of the c\u2010Mpl thrombopoietin receptor through gene disruption and activation","volume":"14","author":"Alexander","year":"1996","journal-title":"Stem Cells"},{"key":"10.1111\/jth.12795_bb0260","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.1182\/blood.V87.6.2162.bloodjournal8762162","article-title":"Deficiencies in progenitor cells of multiple hematopoietic lineages and defective megakaryocytopoiesis in mice lacking the thrombopoietic receptor c\u2010Mpl","volume":"87","author":"Alexander","year":"1996","journal-title":"Blood"},{"key":"10.1111\/jth.12795_bb0265","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.exphem.2008.10.005","article-title":"An ENU\u2010induced recessive mutation in Mpl leads to thrombocytopenia with overdominance","volume":"37","author":"Chan","year":"2009","journal-title":"Exp Hematol"},{"key":"10.1111\/jth.12795_bb0270","doi-asserted-by":"crossref","first-page":"R115","DOI":"10.1186\/cc9064","article-title":"Kinetics of plasmatic cytokines and cystatin\u00a0C during and after hemodialysis in septic shock\u2010related acute renal failure","volume":"14","author":"Mayeur","year":"2010","journal-title":"Crit Care"},{"key":"10.1111\/jth.12795_bb0275","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/nrneph.2011.13","article-title":"Acute kidney injury: what's the prognosis?","volume":"7","author":"Murugan","year":"2011","journal-title":"Nat Rev Nephrol"}],"container-title":["Journal of Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjth.12795","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjth.12795","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1538783622085750?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1538783622085750?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/onlinelibrary.wiley.com\/wol1\/doi\/10.1111\/jth.12795\/fullpdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T03:10:02Z","timestamp":1761534602000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1538783622085750"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["S1538783622085750"],"URL":"https:\/\/doi.org\/10.1111\/jth.12795","relation":{},"ISSN":["1538-7836"],"issn-type":[{"value":"1538-7836","type":"print"}],"subject":[],"published":{"date-parts":[[2015,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel conditional platelet depletion mouse model reveals the importance of platelets in protection against Staphylococcus aureus bacteremia","name":"articletitle","label":"Article Title"},{"value":"Journal of Thrombosis and Haemostasis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1111\/jth.12795","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 International Society on Thrombosis and Haemostasis. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}