{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:41:20Z","timestamp":1773290480288,"version":"3.50.1"},"reference-count":50,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":2138,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100004191","name":"Novo Nordisk","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004191","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008411","name":"Bloodcenter Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL-5P01HL044612"],"award-info":[{"award-number":["HL-5P01HL044612"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL-5P01HL081588"],"award-info":[{"award-number":["HL-5P01HL081588"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL-7R01HL112641"],"award-info":[{"award-number":["HL-7R01HL112641"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL-R01HL102035"],"award-info":[{"award-number":["HL-R01HL102035"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MACC fund"},{"name":"Children's Hospital Foundation"},{"DOI":"10.13039\/100006645","name":"Children's Hospital of Wisconsin","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006645","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jthjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Thrombosis and Haemostasis"],"published-print":{"date-parts":[[2017,1]]},"DOI":"10.1111\/jth.13436","type":"journal-article","created":{"date-parts":[[2016,8,6]],"date-time":"2016-08-06T08:25:07Z","timestamp":1470471907000},"page":"98-109","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"title":["Targeting factor VIII expression to platelets for hemophilia A gene therapy does not induce an apparent thrombotic risk in mice"],"prefix":"10.1016","volume":"15","author":[{"given":"C.K.","family":"Baumgartner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.G.","family":"Mattson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Weiler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.R.","family":"Montgomery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1111\/jth.13436_bb0010","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/0140-6736(92)90874-3","article-title":"Incidence of development of factor VIII and factor IX inhibitors in haemophiliacs","volume":"339","author":"Ehrenforth","year":"1992","journal-title":"Lancet"},{"key":"10.1111\/jth.13436_bb0015","doi-asserted-by":"crossref","first-page":"3365","DOI":"10.1182\/blood-2014-05-577643","article-title":"Toward optimal therapy for inhibitors in hemophilia","volume":"124","author":"Kempton","year":"2014","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0020","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1097\/MOH.0b013e328363c1a1","article-title":"Gene therapy for hemophilia: advancing beyond the first clinical success","volume":"20","author":"Monahan","year":"2013","journal-title":"Curr Opin Hematol"},{"key":"10.1111\/jth.13436_bb0025","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1056\/NEJM200106073442301","article-title":"Nonviral transfer of the gene encoding coagulation factor VIII in patients with severe hemophilia A","volume":"344","author":"Roth","year":"2001","journal-title":"N Engl J Med"},{"key":"10.1111\/jth.13436_bb0030","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1182\/blood-2003-01-0167","article-title":"Phase 1 trial of FVIII gene transfer for severe hemophilia A using a retroviral construct administered by peripheral intravenous infusion","volume":"102","author":"Powell","year":"2003","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0035","doi-asserted-by":"crossref","first-page":"4006","DOI":"10.1182\/blood-2003-05-1519","article-title":"Factor VIII ectopically expressed in platelets: efficacy in hemophilia A treatment","volume":"102","author":"Yarovoi","year":"2003","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0040","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1172\/JCI28416","article-title":"Factor VIII ectopically targeted to platelets is therapeutic in hemophilia A with high\u2010titer inhibitory antibodies","volume":"116","author":"Shi","year":"2006","journal-title":"J Clin Invest"},{"key":"10.1111\/jth.13436_bb0045","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1111\/j.1538-7836.2007.02346.x","article-title":"Lentivirus\u2010mediated platelet\u2010derived factor VIII gene therapy in murine haemophilia A","volume":"5","author":"Shi","year":"2007","journal-title":"J Thromb Haemost"},{"key":"10.1111\/jth.13436_bb0050","doi-asserted-by":"crossref","first-page":"2713","DOI":"10.1182\/blood-2008-02-138214","article-title":"Syngeneic transplantation of hematopoietic stem cells that are genetically modified to express factor VIII in platelets restores hemostasis to hemophilia A mice with preexisting FVIII immunity","volume":"112","author":"Shi","year":"2008","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0055","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1111\/j.1538-7836.2012.04791.x","article-title":"Lentivirus\u2010mediated platelet gene therapy of murine hemophilia A with pre\u2010existing anti\u2010factor VIII immunity","volume":"10","author":"Kuether","year":"2012","journal-title":"J Thromb Haemost"},{"key":"10.1111\/jth.13436_bb0060","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1182\/blood-2013-08-520478","article-title":"Platelet gene therapy corrects the hemophilic phenotype in immunocompromised hemophilia A mice transplanted with genetically manipulated human cord blood stem cells","volume":"123","author":"Shi","year":"2014","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0065","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1111\/jth.12633","article-title":"In vivo enrichment of genetically manipulated platelets corrects the murine hemophilic phenotype and induces immune tolerance even using a low multiplicity of infection","volume":"12","author":"Schroeder","year":"2014","journal-title":"J Thromb Haemost"},{"key":"10.1111\/jth.13436_bb0070","doi-asserted-by":"crossref","first-page":"6114","DOI":"10.1182\/blood-2010-06-293308","article-title":"In vivo efficacy of platelet\u2010delivered, high specific activity factor VIII variants","volume":"116","author":"Greene","year":"2010","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0075","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1111\/j.1538-7836.2008.02992.x","article-title":"Platelet\u2010delivered factor VIII provides limited resistance to anti\u2010factor VIII inhibitors","volume":"6","author":"Gewirtz","year":"2008","journal-title":"J Thromb Haemost"},{"key":"10.1111\/jth.13436_bb0080","doi-asserted-by":"crossref","first-page":"2773","DOI":"10.1038\/ncomms3773","article-title":"Platelet\u2010targeted gene therapy with human factor VIII establishes haemostasis in dogs with haemophilia A","volume":"4","author":"Du","year":"2013","journal-title":"Nat Commun"},{"key":"10.1111\/jth.13436_bb0085","doi-asserted-by":"crossref","first-page":"3706","DOI":"10.1182\/blood-2014-02-555151","article-title":"A conditional knockout mouse model reveals endothelial cells as the predominant and possibly exclusive source of plasma factor VIII","volume":"123","author":"Fahs","year":"2014","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0090","doi-asserted-by":"crossref","first-page":"3697","DOI":"10.1182\/blood-2014-02-554501","article-title":"Murine coagulation factor VIII is synthesized in endothelial cells","volume":"123","author":"Everett","year":"2014","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0095","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1161\/01.ATV.0000095974.95122.EC","article-title":"Platelets in inflammation and thrombosis","volume":"23","author":"Wagner","year":"2003","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"10.1111\/jth.13436_bb0100","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1182\/blood-2008-04-152959","article-title":"Analysis of the spatial and temporal characteristics of platelet\u2010delivered factor VIII\u2010based clots","volume":"112","author":"Neyman","year":"2008","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0105","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1038\/ng0595-119","article-title":"Targeted disruption of the mouse factor VIII gene produces a model of haemophilia A","volume":"10","author":"Bi","year":"1995","journal-title":"Nat Genet"},{"key":"10.1111\/jth.13436_bb0110","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1111\/jth.13169","article-title":"Comparison of platelet\u2010derived and plasma factor VIII efficacy using a novel native whole blood thrombin generation assay","volume":"13","author":"Baumgartner","year":"2015","journal-title":"J Thromb Haemost"},{"key":"10.1111\/jth.13436_bb0115","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1182\/blood-2015-08-662916","article-title":"The immunogenicity of platelet\u2010derived FVIII in hemophilia A mice with or without preexisting anti\u2010FVIII immunity","volume":"127","author":"Chen","year":"2016","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0120","doi-asserted-by":"crossref","first-page":"4222","DOI":"10.1182\/blood.V96.13.4222","article-title":"Spontaneous thrombosis in mice carrying the factor V Leiden mutation","volume":"96","author":"Cui","year":"2000","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0125","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1161\/hq0901.094496","article-title":"Characterization of a mouse model for thrombomodulin deficiency","volume":"21","author":"Weiler","year":"2001","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"10.1111\/jth.13436_bb0130","doi-asserted-by":"crossref","first-page":"4953","DOI":"10.1182\/blood-2010-11-316885","article-title":"Causal relationship between hyperfibrinogenemia, thrombosis, and resistance to thrombolysis in mice","volume":"117","author":"Machlus","year":"2011","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0135","doi-asserted-by":"crossref","first-page":"2774","DOI":"10.2353\/ajpath.2010.100205","article-title":"Interleukin\u20101beta mediates the extra\u2010intestinal thrombosis associated with experimental colitis","volume":"177","author":"Yoshida","year":"2010","journal-title":"Am J Pathol"},{"key":"10.1111\/jth.13436_bb0140","doi-asserted-by":"crossref","first-page":"2217","DOI":"10.1002\/ibd.21593","article-title":"Role of tumor necrosis factor\u2010alpha in the extraintestinal thrombosis associated with colonic inflammation","volume":"17","author":"Yoshida","year":"2011","journal-title":"Inflamm Bowel Dis"},{"key":"10.1111\/jth.13436_bb0145","doi-asserted-by":"crossref","first-page":"S26","DOI":"10.1097\/CCM.0b013e3181c98d21","article-title":"Inflammation and coagulation","volume":"38","author":"Levi","year":"2010","journal-title":"Crit Care Med"},{"key":"10.1111\/jth.13436_bb0150","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1046\/j.1365-2141.2001.03061.x","article-title":"Interleukin 6 and haemostasis","volume":"115","author":"Kerr","year":"2001","journal-title":"Br J Haematol"},{"key":"10.1111\/jth.13436_bb0155","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1038\/369064a0","article-title":"Mutation in blood coagulation factor V associated with resistance to activated protein C","volume":"369","author":"Bertina","year":"1994","journal-title":"Nature"},{"key":"10.1111\/jth.13436_bb0160","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1161\/01.CIR.0000017361.39256.82","article-title":"Lethal perinatal thrombosis in mice resulting from the interaction of tissue factor pathway inhibitor deficiency and factor V Leiden","volume":"105","author":"Eitzman","year":"2002","journal-title":"Circulation"},{"key":"10.1111\/jth.13436_bb0165","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1002\/cyto.10114","article-title":"Flow cytometric detection of activated mouse integrin alphaIIbbeta3 with a novel monoclonal antibody","volume":"48","author":"Bergmeier","year":"2002","journal-title":"Cytometry"},{"key":"10.1111\/jth.13436_bb0170","doi-asserted-by":"crossref","first-page":"1730","DOI":"10.1182\/blood.V78.7.1730.1730","article-title":"Dynamics of leukocyte\u2013platelet adhesion in whole blood","volume":"78","author":"Rinder","year":"1991","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0175","doi-asserted-by":"crossref","first-page":"2702","DOI":"10.1161\/01.ATV.20.12.2702","article-title":"Platelet\u2013leukocyte cross talk in whole blood","volume":"20","author":"Li","year":"2000","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"10.1111\/jth.13436_bb0180","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1016\/S0735-1097(01)01485-1","article-title":"Circulating monocyte\u2013platelet aggregates are an early marker of acute myocardial infarction","volume":"38","author":"Furman","year":"2001","journal-title":"J Am Coll Cardiol"},{"key":"10.1111\/jth.13436_bb0185","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1111\/j.1538-7836.2006.01942.x","article-title":"Elevated levels of soluble fibrin or D\u2010dimer indicate high risk of thrombosis","volume":"4","author":"Wada","year":"2006","journal-title":"J Thromb Haemost"},{"key":"10.1111\/jth.13436_bb0190","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1001\/jama.290.8.1071","article-title":"D\u2010dimer levels and risk of recurrent venous thromboembolism","volume":"290","author":"Eichinger","year":"2003","journal-title":"JAMA"},{"key":"10.1111\/jth.13436_bb0195","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1056\/NEJMoa023153","article-title":"Evaluation of D\u2010dimer in the diagnosis of suspected deep\u2010vein thrombosis","volume":"349","author":"Wells","year":"2003","journal-title":"N Engl J Med"},{"key":"10.1111\/jth.13436_bb0200","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.1161\/01.CIR.90.5.2236","article-title":"Plasma concentration of cross\u2010linked fibrin degradation product (D\u2010dimer) and the risk of future myocardial infarction among apparently healthy men","volume":"90","author":"Ridker","year":"1994","journal-title":"Circulation"},{"key":"10.1111\/jth.13436_bb0205","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1055\/s-0038-1649782","article-title":"Pre\u2010operative plasma levels of thrombin\u2013antithrombin III complexes correlate with the development of venous thrombosis after major hip or knee surgery","volume":"74","author":"Ginsberg","year":"1995","journal-title":"Thromb Haemost"},{"key":"10.1111\/jth.13436_bb0210","doi-asserted-by":"crossref","first-page":"2730","DOI":"10.1111\/j.1538-7836.2005.01639.x","article-title":"Factor V Leiden improves in vivo hemostasis in murine hemophilia models","volume":"3","author":"Schlachterman","year":"2005","journal-title":"J Thromb Haemost"},{"key":"10.1111\/jth.13436_bb0215","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1172\/JCI32878","article-title":"Long\u2010term expression of murine activated factor VII is safe, but elevated levels cause premature mortality","volume":"118","author":"Aljamali","year":"2008","journal-title":"J Clin Invest"},{"key":"10.1111\/jth.13436_bb0220","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1111\/j.1538-7836.2007.02407.x","article-title":"A guide to murine platelet structure, function, assays, and genetic alterations","volume":"5","author":"Jirouskova","year":"2007","journal-title":"J Thromb Haemost"},{"issue":"Suppl. 2","key":"10.1111\/jth.13436_bb0225","first-page":"362","article-title":"Von Willebrand factor propeptide (VWFpp): a marker useful for identifying adverse platelet activation in murine blood phlebotomy samples","volume":"11","author":"Jacobi","year":"2013","journal-title":"J Thromb Haemost"},{"key":"10.1111\/jth.13436_bb0230","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.1182\/blood-2009-02-205914","article-title":"Activation of endothelial intrinsic NF\u2010{kappa}B pathway impairs protein C anticoagulation mechanism and promotes coagulation in endotoxemic mice","volume":"114","author":"Song","year":"2009","journal-title":"Blood"},{"key":"10.1111\/jth.13436_bb0235","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1161\/CIRCULATIONAHA.112.000966","article-title":"Hydrogen peroxide promotes aging\u2010related platelet hyperactivation and thrombosis","volume":"127","author":"Dayal","year":"2013","journal-title":"Circulation"},{"key":"10.1111\/jth.13436_bb0240","first-page":"169","article-title":"Increased platelet and leukocyte activation as contributing mechanisms for thrombosis in essential thrombocythemia and correlation with the JAK2 mutational status","volume":"91","author":"Arellano\u2010Rodrigo","year":"2006","journal-title":"Haematologica"},{"key":"10.1111\/jth.13436_bb0245","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1080\/095371001317126392","article-title":"Influence of the genetic background on platelet function, microparticle and thrombin generation in the common laboratory mouse","volume":"12","author":"Zumbach","year":"2001","journal-title":"Platelets"},{"key":"10.1111\/jth.13436_bb0250","doi-asserted-by":"crossref","first-page":"2102","DOI":"10.1111\/jth.12749","article-title":"Apoptotic effects of platelet factor VIII on megakaryopoiesis: implications for a modified human FVIII for platelet\u2010based gene therapy","volume":"12","author":"Greene","year":"2014","journal-title":"J Thromb Haemost"},{"key":"10.1111\/jth.13436_bb0255","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1160\/TH08-04-0242","article-title":"Altered bioavailability of platelet\u2010derived factor VIII during thrombocytosis reverses phenotypic efficacy in haemophilic mice","volume":"100","author":"Damon","year":"2008","journal-title":"Thromb Haemost"}],"container-title":["Journal of Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjth.13436","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjth.13436","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S153878362203135X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S153878362203135X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/onlinelibrary.wiley.com\/wol1\/doi\/10.1111\/jth.13436\/fullpdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T03:16:05Z","timestamp":1761534965000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S153878362203135X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["S153878362203135X"],"URL":"https:\/\/doi.org\/10.1111\/jth.13436","relation":{},"ISSN":["1538-7836"],"issn-type":[{"value":"1538-7836","type":"print"}],"subject":[],"published":{"date-parts":[[2017,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Targeting factor VIII expression to platelets for hemophilia A gene therapy does not induce an apparent thrombotic risk in mice","name":"articletitle","label":"Article Title"},{"value":"Journal of Thrombosis and Haemostasis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1111\/jth.13436","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 International Society on Thrombosis and Haemostasis. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}