{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:42:48Z","timestamp":1790836968793,"version":"4.1.0"},"reference-count":24,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T00:00:00Z","timestamp":1667433600000},"content-version":"vor","delay-in-days":763,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01Hl68130 [JEI]"],"award-info":[{"award-number":["R01Hl68130 [JEI]"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["T32HL066987\u201016 [ARW]"],"award-info":[{"award-number":["T32HL066987\u201016 [ARW]"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["K01DK111515 [KRM]"],"award-info":[{"award-number":["K01DK111515 [KRM]"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM122547 [AEC]"],"award-info":[{"award-number":["R35GM122547 [AEC]"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jthjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Thrombosis and Haemostasis"],"published-print":{"date-parts":[[2020,10]]},"DOI":"10.1111\/jth.15012","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T04:45:34Z","timestamp":1594701934000},"page":"2701-2711","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["High\u2010content, label\u2010free analysis of proplatelet production from megakaryocytes"],"prefix":"10.1016","volume":"18","author":[{"given":"Shauna L.","family":"French","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakrith","family":"Vijey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle W.","family":"Karhohs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian R.","family":"Wilkie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1099-5673","authenticated-orcid":false,"given":"Lillian J.","family":"Horin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjana","family":"Ray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Posorske","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne E.","family":"Carpenter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2155-1050","authenticated-orcid":false,"given":"Kellie R.","family":"Machlus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6547-9663","authenticated-orcid":false,"suffix":"Jr.","given":"Joseph E.","family":"Italiano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6","key":"10.1111\/jth.15012_bb0010","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1083\/jcb.201304054","article-title":"The incredible journey: from megakaryocyte development to platelet formation","volume":"201","author":"Machlus","year":"2013","journal-title":"J Cell Biol"},{"key":"10.1111\/jth.15012_bb0015","first-page":"153","article-title":"Drug\u2010induced thrombocytopenia: pathogenesis, evaluation, and management. Hematology American society of hematology education","author":"George","year":"2009","journal-title":"Program"},{"issue":"Suppl 1","key":"10.1111\/jth.15012_bb0020","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1111\/j.1538-7836.2007.02487.x","article-title":"Mechanics of proplatelet elaboration","volume":"5","author":"Italiano","year":"2007","journal-title":"J Thromb Haemost"},{"issue":"6","key":"10.1111\/jth.15012_bb0025","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1083\/jcb.147.6.1299","article-title":"Blood platelets are assembled principally at the ends of proplatelet processes produced by differentiated megakaryocytes","volume":"147","author":"Italiano","year":"1999","journal-title":"J Cell Biol"},{"issue":"5","key":"10.1111\/jth.15012_bb0030","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1182\/blood-2014-09-600858","article-title":"Microtubule sliding drives proplatelet elongation and is dependent on cytoplasmic dynein","volume":"125","author":"Bender","year":"2015","journal-title":"Blood"},{"issue":"4","key":"10.1111\/jth.15012_bb0035","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1083\/jcb.201006102","article-title":"Cytoskeletal mechanics of proplatelet maturation and platelet release","volume":"191","author":"Thon","year":"2010","journal-title":"J Cell Biol"},{"issue":"5845","key":"10.1111\/jth.15012_bb0040","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1126\/science.1146304","article-title":"Dynamic visualization of thrombopoiesis within bone marrow","volume":"317","author":"Junt","year":"2007","journal-title":"Science"},{"issue":"9","key":"10.1111\/jth.15012_bb0045","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1111\/jth.14544","article-title":"New insights into cytoskeletal remodeling during platelet production","volume":"17","author":"Ghalloussi","year":"2019","journal-title":"J Thromb Haemost"},{"issue":"6","key":"10.1111\/jth.15012_bb0050","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1080\/09537104.2018.1492107","article-title":"In vitro culture of murine megakaryocytes from fetal liver\u2010derived hematopoietic stem cells","volume":"29","author":"Vijey","year":"2018","journal-title":"Platelets"},{"issue":"4","key":"10.1111\/jth.15012_bb0055","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1080\/09537104.2018.1430359","article-title":"A novel method for automated assessment of megakaryocyte differentiation and proplatelet formation","volume":"29","author":"Salzmann","year":"2018","journal-title":"Platelets"},{"issue":"5","key":"10.1111\/jth.15012_bb0060","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1177\/1087057114528537","article-title":"Increasing the content of high\u2010content screening: an overview","volume":"19","author":"Singh","year":"2014","journal-title":"J Biomol Screen"},{"issue":"10","key":"10.1111\/jth.15012_bb0065","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1182\/blood-2012-04-420968","article-title":"High\u2010content live\u2010cell imaging assay used to establish mechanism of trastuzumab emtansine (T\u2010DM1)\u2013mediated inhibition of platelet production","volume":"120","author":"Thon","year":"2012","journal-title":"Blood"},{"issue":"15","key":"10.1111\/jth.15012_bb0070","doi-asserted-by":"crossref","first-page":"4106","DOI":"10.1182\/blood-2010-07-293092","article-title":"Developmental differences in megakaryocytopoiesis are associated with up\u2010regulated TPO signaling through mTOR and elevated GATA\u20101 levels in neonatal megakaryocytes","volume":"117","author":"Liu","year":"2011","journal-title":"Blood"},{"key":"10.1111\/jth.15012_bb0075","doi-asserted-by":"crossref","unstructured":"Sommer C, Str\u00e4hle C, K\u00f6the U, Hamprecht FA. ilastik: interactive learning and segmentation toolkit. Proc Eighth IEEE International Symposium on Biomedical Imaging. 2011:230\u2010233.","DOI":"10.1109\/ISBI.2011.5872394"},{"issue":"12","key":"10.1111\/jth.15012_bb0080","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1038\/s41592-019-0582-9","article-title":"ilastik: interactive machine learning for (bio)image analysis","volume":"16","author":"Berg","year":"2019","journal-title":"Nat Methods"},{"issue":"11","key":"10.1111\/jth.15012_bb0085","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1182\/blood-2015-08-663146","article-title":"Synthesis and dephosphorylation of MARCKS in the late stages of megakaryocyte maturation drive proplatelet formation","volume":"127","author":"Machlus","year":"2016","journal-title":"Blood"},{"issue":"Pt 1","key":"10.1111\/jth.15012_bb0090","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1242\/jcs.97.1.59","article-title":"Blood platelet formation in vitro. The role of the cytoskeleton in megakaryocyte fragmentation","volume":"97","author":"Tablin","year":"1990","journal-title":"J Cell Sci"},{"issue":"4","key":"10.1111\/jth.15012_bb0095","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0196400","article-title":"Megakaryocyte lineage development is controlled by modulation of protein acetylation","volume":"13","author":"Bartels","year":"2018","journal-title":"PLoS One"},{"issue":"13","key":"10.1111\/jth.15012_bb0100","doi-asserted-by":"crossref","first-page":"3658","DOI":"10.1182\/blood-2010-11-318055","article-title":"Deciphering the molecular and biologic processes that mediate histone deacetylase inhibitor\u2010induced thrombocytopenia","volume":"117","author":"Bishton","year":"2011","journal-title":"Blood"},{"issue":"5","key":"10.1111\/jth.15012_bb0105","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1080\/15592294.2016.1265710","article-title":"Bromodomain inhibitors and cancer therapy: From structures to applications","volume":"12","author":"Perez\u2010Salvia","year":"2017","journal-title":"Epigenetics"},{"key":"10.1111\/jth.15012_bb0110","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.ymeth.2015.12.002","article-title":"Quantifying co\u2010cultured cell phenotypes in high\u2010throughput using pixel\u2010based classification","volume":"96","author":"Logan","year":"2016","journal-title":"Methods"},{"issue":"1","key":"10.1111\/jth.15012_bb0115","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/jcp.10035","article-title":"Roads to polyploidy: the megakaryocyte example","volume":"190","author":"Ravid","year":"2002","journal-title":"J Cell Physiol"},{"key":"10.1111\/jth.15012_bb0120","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1182\/blood-2014-05-574913","article-title":"Platelet bioreactor\u2010on\u2010a\u2010chip","volume":"124","author":"Thon","year":"2014","journal-title":"Blood"},{"key":"10.1111\/jth.15012_bb0125","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.cell.2018.06.011","article-title":"Turbulence activates platelet biogenesis to enable clinical scale ex vivo production","volume":"174","author":"Ito","year":"2018","journal-title":"Cell"}],"container-title":["Journal of Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjth.15012","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jth.15012","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jth.15012","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1538783622011813?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1538783622011813?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jth.15012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T03:25:36Z","timestamp":1761535536000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1538783622011813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["S1538783622011813"],"URL":"https:\/\/doi.org\/10.1111\/jth.15012","relation":{},"ISSN":["1538-7836"],"issn-type":[{"value":"1538-7836","type":"print"}],"subject":[],"published":{"date-parts":[[2020,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High\u2010content, label\u2010free analysis of proplatelet production from megakaryocytes","name":"articletitle","label":"Article Title"},{"value":"Journal of Thrombosis and Haemostasis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1111\/jth.15012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2020 International Society on Thrombosis and Haemostasis. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}