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One hundred and thirty\u2010four volunteers were evaluated (mean age 21\u2009\u00b1\u20092\u2009years). Body composition was calculated through skinfold thickness: supra\u2010iliac, biceps, and triceps, sub\u2010scapular. Body fat percentage (BF%) was used to classify subjects according to fat tissue accumulation: normal\u2010weight (<jats:italic>n<\/jats:italic>\u2009=\u200937, 19 females, 18 males), overweight (<jats:italic>n<\/jats:italic>\u2009=\u200942, 30 females, 12 males), and obese (<jats:italic>n<\/jats:italic>\u2009=\u200955, 42 females, 13 males). Saliva samples were collected 30\u2009min after awakening to determine salivary levels of 17\u2010beta\u2010estradiol. For salivary cortisol and alpha\u2010amylase activity (sAA) three samples were obtained, just after awakening, 30\u2009min after awakening and at bedtime. Oral contraceptive intake was considered for the female group. The results showed that overweight and obese females using oral contraceptive presented lower levels of 17\u2010\u03b2\u2010estradiol than normal\u2010weight females. In overweight and obese males, sAA levels were higher 30\u2009min post\u2010awakening when compared with the normal\u2010weight group. The comparison of sAA levels within time showed no difference for males; obese females showed significant higher values at bedtime than 30 min post\u2010awakening. The salivary cortisol concentration showed higher values at morning decreasing significantly at bedtime for all groups. Concluding, differences in 17\u2010\u03b2\u2010estradiol and sAA levels were found in females and males, respectively, according to body fat accumulation, showing the usefulness of salivary biomarkers in the study of systemic conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Practical applications<\/jats:title><jats:p>Saliva is an advantageous biological fluid in innovative methods for diagnosis. Besides being a noninvasive method, salivary steroid measurements have the potential to provide a convenient assessment of serum free steroid concentrations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jtxs.12384","type":"journal-article","created":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T13:03:38Z","timestamp":1545138218000},"page":"45-52","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Salivary alpha\u2010amylase and hormones levels of young adults with different body composition"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2829-0508","authenticated-orcid":false,"given":"B\u00e1rbara de Lima","family":"Lucas","sequence":"first","affiliation":[{"name":"Health Science Special Unit, Federal University of Goias  Jata\u00ed Goi\u00e1s Brazil"},{"name":"Department of Pediatric Dentistry, Piracicaba Dental School University of Campinas  Piracicaba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u00eds de Souza","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Department of Dentistry Institute of Life Sciences, Federal University of Juiz de Fora  Governador Valadares Minas Gerais Brazil"},{"name":"Department of Pediatric Dentistry, Piracicaba Dental School University of Campinas  Piracicaba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8703-2272","authenticated-orcid":false,"given":"Paula Midori","family":"Castelo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Federal University of S\u00e3o Paulo (UNIFESP)  Diadema S\u00e3o Paulo Brazil"},{"name":"Department of Pediatric Dentistry, Piracicaba Dental School University of Campinas  Piracicaba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7546-5262","authenticated-orcid":false,"given":"Maria Beatriz Duarte","family":"Gavi\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Piracicaba Dental School University of Campinas  Piracicaba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.20083"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2009.01.003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.5604\/17322693.1176778"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10266-013-0103-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1258\/000456307780480954"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19740060"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000140086.88453.9A"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dem432"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1100\/tsw.2010.38"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365510510025773"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/geronb\/gbn028"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/obr.12019"},{"key":"e_1_2_7_14_1","volume-title":"Anthropometric standardizing reference manual","author":"Gordon C. 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