{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:03:59Z","timestamp":1761581039202,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,11,15]],"date-time":"2012-11-15T00:00:00Z","timestamp":1352937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["KFO129"],"award-info":[{"award-number":["KFO129"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We characterized the early dynamics of hepatitis <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> virus (<jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>) quasispecies evolution during the first weeks of antiviral therapy with low\u2010to\u2010moderate genetic barrier antiviral drugs and associated these data with antiviral response patterns. Fifteen chronic hepatitis <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> patients (men, 10; mean age, 34; <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>e<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g positive, 6) who received lamivudine or telbivudine for at least 52\u00a0weeks were included. <jats:styled-content style=\"fixed-case\">HBV DNA<\/jats:styled-content> was extracted from serum, and a 910\u2010bp fragment covering domains A\u2013F of the reverse transcriptase region was amplified, cloned and sequenced. Parameters of quasispecies heterogeneity, genetic diversity and complexity were calculated and were correlated with complete virologic response, defined as undetectable <jats:styled-content style=\"fixed-case\">HBV DNA<\/jats:styled-content> at week 52. Nine patients achieved complete virologic response during the observational period. While baseline <jats:styled-content style=\"fixed-case\">HBV DNA<\/jats:styled-content> levels and <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>eAg status were associated with virologic response, baseline quasispecies complexity and diversity of responders showed no significant difference to those of nonresponders (<jats:italic>P\u00a0<\/jats:italic>&gt;\u00a00.05). However, at week 4, quasispecies complexity of nonresponders was significantly higher compared with that of responders on the nucleotide level (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.01) and the aa level (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.04). The number of synonymous substitutions per synonymous site dropped significantly in responders at week 4 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.04), while there was no difference in nonresponders. The <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> quasispecies complexity at the early stage of antiviral therapy (week 4) with the low\u2010to\u2010moderate genetic barrier nucleoside analogs lamivudine or telbivudine was associated with subsequent virologic response. Further studies are needed to confirm <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> quasispecies evolution as additional predictive marker for beneficial treatment outcome.<\/jats:p>","DOI":"10.1111\/jvh.12013","type":"journal-article","created":{"date-parts":[[2012,11,15]],"date-time":"2012-11-15T22:49:12Z","timestamp":1353019752000},"page":"234-239","source":"Crossref","is-referenced-by-count":18,"title":["Dynamics of hepatitis <scp>B<\/scp> virus quasispecies heterogeneity and virologic response in patients receiving low\u2010to\u2010moderate genetic barrier nucleoside analogs"],"prefix":"10.1111","volume":"20","author":[{"given":"J.","family":"Peveling\u2010Oberhag","sequence":"first","affiliation":[{"name":"Department of Internal Medicine 1 J.W. Goethe\u2010University Hospital Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Institute for Biostatistics and Mathmatical Modelling J.W. Goethe\u2010University Hospital Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Kronenberger","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine 1 J.W. Goethe\u2010University Hospital Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Farnik","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine 1 J.W. Goethe\u2010University Hospital Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Susser","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine 1 J.W. Goethe\u2010University Hospital Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Sarrazin","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine 1 J.W. Goethe\u2010University Hospital Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Zeuzem","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine 1 J.W. Goethe\u2010University Hospital Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.\u2010P.","family":"Hofmann","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine 1 J.W. Goethe\u2010University Hospital Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,11,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.295.1.65"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1177\/135965350601100614","article-title":"Hepatitis B virus replication and liver disease progression: the impact of antiviral therapy","volume":"11","author":"Liaw YF","year":"2006","journal-title":"Antivir Ther"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9071(200007)61:3<362::AID-JMV14>3.0.CO;2-I"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2003.50013"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.08.025"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.09.020"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.10.011"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa066422"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043470"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040431"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.10.026"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.27563"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2008.12.019"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/368083"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2000.19619"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.08.013"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21290"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-001-0399-7"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/16.6.562"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.1092"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbn017"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2008.12.018"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.226225"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2012.02.010"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21513"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-008-9080-3"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2009.04.003"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-008-9049-2"}],"container-title":["Journal of Viral Hepatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjvh.12013","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjvh.12013","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jvh.12013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T01:26:55Z","timestamp":1698629215000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jvh.12013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,15]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1111\/jvh.12013"],"URL":"https:\/\/doi.org\/10.1111\/jvh.12013","archive":["Portico"],"relation":{},"ISSN":["1352-0504","1365-2893"],"issn-type":[{"type":"print","value":"1352-0504"},{"type":"electronic","value":"1365-2893"}],"subject":[],"published":{"date-parts":[[2012,11,15]]}}}