{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:13:11Z","timestamp":1793059991313,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,8,13]],"date-time":"2013-08-13T00:00:00Z","timestamp":1376352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["SFB 852\/1"],"award-info":[{"award-number":["SFB 852\/1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Hepatitis <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> is an increasingly reported disease in industrialized countries. Studies on the replication cycle of hepatitis <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> virus (<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>) are hampered due to the lack of efficient and robust cell culture systems for this virus. We describe the successful isolation of <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> derived from a chronically infected kidney transplant patient held under immunosuppressive therapy. Inoculation of serum sample 47832 onto the human lung carcinoma cell line <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>549 resulted in the replication of the virus as shown by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. This novel human\u2010derived <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> strain is closely related to a wild boar\u2010derived genotype 3 strain, which did not replicate in <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>549 cells. It carries a 186 nucleotide insertion in the hypervariable <jats:styled-content style=\"fixed-case\">ORF<\/jats:styled-content>1\u2010region, derived from two parts of its <jats:styled-content style=\"fixed-case\">ORF<\/jats:styled-content>1. By passaging of the infected cells, a cell line continuously producing <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> particles was generated as demonstrated by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, immuno\u2010electron microscopy, density gradient centrifugation and immunohistochemistry. Replication of the produced virus was demonstrated after its inoculation onto fresh <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>549 cells and two consecutive passages, whereas heating at 65\u00a0\u00b0C for 2\u00a0min abolished its infectivity. Several point mutations scattered along the whole genome were present in the <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> strain from the second passage; however, the <jats:styled-content style=\"fixed-case\">ORF<\/jats:styled-content>1 insertion was still present. Previously, cell culture isolation of two other <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> strains carrying insertions in their hypervariable regions, but originating from human ribosomal protein genes, has been described. The findings may indicate that cell culture adaptation of is mostly dependent on the length and position of the insertion, rather than from the sequence itself.<\/jats:p>","DOI":"10.1111\/jvh.12157","type":"journal-article","created":{"date-parts":[[2013,8,14]],"date-time":"2013-08-14T04:53:07Z","timestamp":1376455987000},"page":"447-456","source":"Crossref","is-referenced-by-count":123,"title":["An <scp>ORF<\/scp>1\u2010rearranged hepatitis <scp>E<\/scp> virus derived from a chronically infected patient efficiently replicates in cell culture"],"prefix":"10.1111","volume":"21","author":[{"given":"R.","family":"Johne","sequence":"first","affiliation":[{"name":"Department of Biological Safety Federal Institute for Risk Assessment Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Reetz","sequence":"additional","affiliation":[{"name":"Department of Biological Safety Federal Institute for Risk Assessment Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. G.","family":"Ulrich","sequence":"additional","affiliation":[{"name":"Friedrich\u2010Loeffler\u2010Institut Federal Research Institute for Animal Health Institute for Novel and Emerging Infectious Diseases Greifswald\u2010Insel Riems Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Machnowska","sequence":"additional","affiliation":[{"name":"Department of Biological Safety Federal Institute for Risk Assessment Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Sachsenr\u00f6der","sequence":"additional","affiliation":[{"name":"Department of Biological Safety Federal Institute for Risk Assessment Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Nickel","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Intensive Care Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Campus Virchow\u2010Klinikum Berlin 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