{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T05:42:34Z","timestamp":1770961354669,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,8,15]],"date-time":"2013-08-15T00:00:00Z","timestamp":1376524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Combination therapy with pegylated interferon (pegIFN) plus ribavirin (<jats:styled-content style=\"fixed-case\">RBV<\/jats:styled-content>) is the standard of care for chronic hepatitis <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>. One of the major treatment\u2010related side effects is anaemia, attributed to <jats:styled-content style=\"fixed-case\">RBV<\/jats:styled-content>\u2010induced haemolysis. However, haemolysis biomarkers are not present in all patients supporting the existence of other pathogenetic mechanisms. We studied the role of <jats:styled-content style=\"fixed-case\">RBV<\/jats:styled-content> in inducing haemolysis and its effects on erythropoiesis. In 18 hepatitis <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> virus (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>) genotype 2 patients treated with pegIFN\u2010alpha\u20102a (180\u00a0mcg\/week) plus <jats:styled-content style=\"fixed-case\">RBV<\/jats:styled-content> (800\u00a0mg\/day) for 24\u00a0weeks and in 10 hepatitis B virus (<jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>) patients treated with pegIFN\u2010alpha\u20102a (180\u00a0mcg\/week) for 48\u00a0weeks, haemolysis was assessed by serum <jats:styled-content style=\"fixed-case\">LDH<\/jats:styled-content>, haptoglobin and reticulocyte count. Erythropoiesis was evaluated both <jats:italic>ex vivo<\/jats:italic>, analysing the clonogenic activity of patients' erythroid progenitors, as well as <jats:italic>in vitro<\/jats:italic> adding pegIFN and <jats:styled-content style=\"fixed-case\">RBV<\/jats:styled-content> to liquid cultures obtained from CD34+ cells of healthy volunteers. The majority of patients developed anaemia; the week 4 mean haemoglobin decrease was greater in <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> than in <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> patients (1.7 <jats:italic>vs<\/jats:italic> 0.47\u00a0g\/dL, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.01). Only three <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> patients (17%) and no <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> patients showed signs of haemolysis. The 15 nonhaemolytic <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> patients and all <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> patients showed a delay in erythroid differentiation, with a reduction in colony number and a relative increase in undifferentiated colony percentage. Haemolytic <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> patients had an increase in colony number at week 4 of therapy. <jats:italic>In vitro,<\/jats:italic> erythroid cell proliferation and differentiation were inhibited by both pegIFN and <jats:styled-content style=\"fixed-case\">RBV<\/jats:styled-content>. Both pegIFN and <jats:styled-content style=\"fixed-case\">RBV<\/jats:styled-content> have an inhibitory effect on erythroid proliferation and differentiation.<\/jats:p>","DOI":"10.1111\/jvh.12158","type":"journal-article","created":{"date-parts":[[2013,8,15]],"date-time":"2013-08-15T14:35:26Z","timestamp":1376577326000},"page":"416-423","source":"Crossref","is-referenced-by-count":12,"title":["Ribavirin suppresses erythroid differentiation and proliferation in chronic hepatitis <scp>C<\/scp> patients"],"prefix":"10.1111","volume":"21","author":[{"given":"L.","family":"Ronzoni","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences and Community Fondazione IRCCS C\u00e0 Granda Ospedale Maggiore Policlinico University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Aghemo","sequence":"additional","affiliation":[{"name":"A.M. and A. Migliavacca Center for Liver Disease First Division of Gastroenterology Fondazione IRCCS C\u00e0 Granda Ospedale Maggiore Policlinico University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. G.","family":"Rumi","sequence":"additional","affiliation":[{"name":"Division of Hepatology Ospedale San Giuseppe University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Prati","sequence":"additional","affiliation":[{"name":"A.M. and A. Migliavacca Center for Liver Disease First Division of Gastroenterology Fondazione IRCCS C\u00e0 Granda Ospedale Maggiore Policlinico University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Colancecco","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Community Fondazione IRCCS C\u00e0 Granda Ospedale Maggiore Policlinico University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Porretti","sequence":"additional","affiliation":[{"name":"Cytometry Laboratory Department of Regenerative Medicine Fondazione IRCCS C\u00e0 Granda Ospedale Maggiore Policlinico University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Monico","sequence":"additional","affiliation":[{"name":"A.M. and A. Migliavacca Center for Liver Disease First Division of Gastroenterology Fondazione IRCCS C\u00e0 Granda Ospedale Maggiore Policlinico University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Colombo","sequence":"additional","affiliation":[{"name":"A.M. and A. Migliavacca Center for Liver Disease First Division of Gastroenterology Fondazione IRCCS C\u00e0 Granda Ospedale Maggiore Policlinico University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. D.","family":"Cappellini","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Community Fondazione IRCCS C\u00e0 Granda Ospedale Maggiore Policlinico University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24641"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2011.02.023"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04082"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.03.037"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0807650"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.5812\/kowsar.1735143X.3755"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2011.21"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/he.2000.5789"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08825"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.06.016"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21853"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.2174\/092986730607220401122851","article-title":"Consequences of IMP dehydrogenase inhibition and its relationship to cancer and apoptosis","volume":"6","author":"Jayaram HN","year":"1999","journal-title":"Curr Med Chem"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.07.027"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2007.07.006"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi970226n"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.9.1.15"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60934-8"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1012912"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1010494"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1009482"},{"issue":"4","key":"e_1_2_7_22_1","first-page":"76A","article-title":"Once daily PSI\u20107977 plus Rbv: pegylated interferon\u2010alfa not required for complete rapid viral response in treatment\u2010naive patients with HCV GT2 or GT3 [Abstract]","volume":"54","author":"Gane EJ","year":"2011","journal-title":"Hepatology"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000168410"}],"container-title":["Journal of Viral Hepatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjvh.12158","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjvh.12158","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jvh.12158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T23:01:13Z","timestamp":1696201273000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jvh.12158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,15]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1111\/jvh.12158"],"URL":"https:\/\/doi.org\/10.1111\/jvh.12158","archive":["Portico"],"relation":{},"ISSN":["1352-0504","1365-2893"],"issn-type":[{"value":"1352-0504","type":"print"},{"value":"1365-2893","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,15]]}}}