{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:55:38Z","timestamp":1790128538759,"version":"4.0.1"},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,11,6]],"date-time":"2013-11-06T00:00:00Z","timestamp":1383696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010253","name":"UBACyT","doi-asserted-by":"crossref","award":["20020110100034"],"award-info":[{"award-number":["20020110100034"]}],"id":[{"id":"10.13039\/501100010253","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>When intrafamilial transmission of hepatitis <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> virus (<jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>) occurs, a virus with the same characteristics interacts with diverse hosts' immune systems and may thus result in different mutations to escape immune pressure. In this study, the <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> genomic characterization was assessed longitudinally after intrafamilial transmission using nucleotide sequence data of phylogenetic and mutational analyses, including those obtained by deep\u2010sequencing for the first time. Furthermore, <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>e<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g\u2010anti\u2010<jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>e profile and variability of <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> core\u2010derived epitopes were also evaluated. Strong evidence was obtained from intrafamilial transmission of <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> genotype <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>1 by phylogenetic inferences. <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> isolates exhibited high degree (~99%) of genomic conservation for almost 20\u00a0years, when patients were persistently <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>e<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g positive with normal amino transferase levels. This identity remained high among immune\u2010tolerant siblings. In contrast, it diminished significantly (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.02) when the mother cleared <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>e<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g (immune clearance phase). By deep\u2010sequencing, the quantitative analysis of the dynamics of basal core promoter (<jats:styled-content style=\"fixed-case\">BCP<\/jats:styled-content>) (<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>1762<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> G<\/jats:styled-content>1764<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>;<jats:styled-content style=\"fixed-case\"> A<\/jats:styled-content>1766<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>;<jats:styled-content style=\"fixed-case\"> T<\/jats:styled-content>1773<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>; 8\u2010bp deletion; and other) and precore (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>1896<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>) variants among <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> isolates from family members exhibited differences during the follow\u2010up. However, only those from the mother showed amino acid variations at core protein that would impair their <jats:styled-content style=\"fixed-case\">MHC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> binding. Hence, when intrafamilial transmission occurs, <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> was highly conserved under the immune\u2010tolerant phase, but it exhibited mutations more frequently during the immune clearance phase. The analysis of the <jats:styled-content style=\"fixed-case\">HBV BCP<\/jats:styled-content> and precore mutants after intrafamilial <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> transmission contributes to a better understanding of how they evolve over time.<\/jats:p>","DOI":"10.1111\/jvh.12196","type":"journal-article","created":{"date-parts":[[2013,11,7]],"date-time":"2013-11-07T03:30:40Z","timestamp":1383795040000},"page":"650-661","source":"Crossref","is-referenced-by-count":12,"title":["Hepatitis <scp>B<\/scp> virus depicts a high degree of conservation during the immune\u2010tolerant phase in familiarly transmitted chronic hepatitis <scp>B<\/scp> infection: deep\u2010sequencing and phylogenetic analysis"],"prefix":"10.1111","volume":"21","author":[{"given":"M.","family":"Sede","sequence":"first","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"},{"name":"Instituto de Investigaciones Biom\u00e9dicas en Retrovirus y Sida (INBIRS) Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lopez\u2010Ledesma","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"},{"name":"C\u00e1tedra de Virolog\u00eda Facultad de Farmacia y Bioqu\u00edmica Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Frider","sequence":"additional","affiliation":[{"name":"Servicio de Hepatolog\u00eda Hospital \u2018Dr. Cosme Argerich\u2019 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Pozzati","sequence":"additional","affiliation":[{"name":"Servicio de Hepatolog\u00eda Hospital \u2018Dr. Cosme Argerich\u2019 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. H.","family":"Campos","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"},{"name":"C\u00e1tedra de Virolog\u00eda Facultad de Farmacia y Bioqu\u00edmica Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Flichman","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"},{"name":"C\u00e1tedra de Virolog\u00eda Facultad de Farmacia y Bioqu\u00edmica Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Quarleri","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"},{"name":"Instituto de Investigaciones Biom\u00e9dicas en Retrovirus y Sida (INBIRS) 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