{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:33:31Z","timestamp":1758710011288,"version":"3.37.3"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T00:00:00Z","timestamp":1395878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Spanish Ministry of Science Innovation","award":["BFU2010-15194","SAF2010-21617"],"award-info":[{"award-number":["BFU2010-15194","SAF2010-21617"]}]},{"name":"Red Tematica de Investigacion Cooperativa en SIDA","award":["RD12\/0017"],"award-info":[{"award-number":["RD12\/0017"]}]},{"DOI":"10.13039\/100015670","name":"European AIDS Treatment Network","doi-asserted-by":"crossref","award":["LSHP-CT-2006-037570"],"award-info":[{"award-number":["LSHP-CT-2006-037570"]}],"id":[{"id":"10.13039\/100015670","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The growing number of cases of acute hepatitis C (<jats:styled-content style=\"fixed-case\">AHC<\/jats:styled-content>) infections among human immunodeficiency virus type 1 (<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101)\u2010positive men who have sex with men (<jats:styled-content style=\"fixed-case\">MSM<\/jats:styled-content>) in the last 10\u00a0years has promoted the search for predictors of <jats:styled-content style=\"fixed-case\">AHC<\/jats:styled-content> clearance as well as for epidemiological networks of viral transmission. We characterized the diversity and catalytic efficiency of <jats:styled-content style=\"fixed-case\">HCV NS<\/jats:styled-content>3\/4A protease quasispecies in <jats:styled-content style=\"fixed-case\">AHC<\/jats:styled-content> patients coinfected with <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101. Plasma samples obtained at <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> diagnosis from 18 <jats:styled-content style=\"fixed-case\">MSM HIV<\/jats:styled-content>\u2010coinfected patients with <jats:styled-content style=\"fixed-case\">AHC<\/jats:styled-content> were studied. Five <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> monoinfected patient samples with <jats:styled-content style=\"fixed-case\">AHC<\/jats:styled-content> were also investigated. An average of 39 clones from each sample was analysed. The catalytic efficiency of the dominant quasispecies (i.e. the most abundant) from each quasispecies was also assayed for mitochondrial antiviral signalling protein (<jats:styled-content style=\"fixed-case\">MAVS<\/jats:styled-content>) cleavage. Phylogenetic analysis identified two clusters of patients with highly related viruses, suggesting a common source of <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> infection. None of the 18 <jats:styled-content style=\"fixed-case\">MSM HIV<\/jats:styled-content>\u2010coinfected patients spontaneously cleared <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>, although 78% of the treated patients achieved a sustained virological response after early treatment with pegylated interferon (peg<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>) plus ribavirin (<jats:styled-content style=\"fixed-case\">RBV<\/jats:styled-content>). The synonymous\u2010nonsynonymous (ds\/dn) mutation ratio, a marker of selective pressure, was higher in <jats:styled-content style=\"fixed-case\">AHC<\/jats:styled-content> compared to 26 <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101\u2010infected men with genotype 1a chronic hepatitis C (<jats:styled-content style=\"fixed-case\">CHC<\/jats:styled-content>) (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.0001). <jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>3\/4A proteases from <jats:styled-content style=\"fixed-case\">AHC<\/jats:styled-content> patients also exhibited higher catalytic efficiency compared to <jats:styled-content style=\"fixed-case\">CHC<\/jats:styled-content> patients (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.0001). No differences were found when ds\/dn mutation ratios and <jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>3\/4A protease catalytic efficiencies from <jats:styled-content style=\"fixed-case\">AHC HIV<\/jats:styled-content>\u2010coinfected patients were compared with <jats:styled-content style=\"fixed-case\">AHC<\/jats:styled-content> monoinfected patients. The presence of epidemiological networks of <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> transmission was confirmed among <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101\u2010positive <jats:styled-content style=\"fixed-case\">MSM<\/jats:styled-content>. In addition, substantial genetic diversity was demonstrated in <jats:styled-content style=\"fixed-case\">AHC<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>3\/4A protease efficiency cleaving <jats:styled-content style=\"fixed-case\">MAVS<\/jats:styled-content> may be associated with virus transmission and response to peg<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">RBV<\/jats:styled-content> treatment.<\/jats:p>","DOI":"10.1111\/jvh.12254","type":"journal-article","created":{"date-parts":[[2014,3,28]],"date-time":"2014-03-28T03:56:59Z","timestamp":1395979019000},"source":"Crossref","is-referenced-by-count":9,"title":["Hepatitis C virus <scp>NS<\/scp>3\/4A quasispecies diversity in acute hepatitis C infection in <scp>HIV<\/scp>\u20101 co\u2010infected patients"],"prefix":"10.1111","volume":"21","author":[{"given":"M.","family":"Nevot","sequence":"first","affiliation":[{"name":"Fundaci\u00f3 irsiCaixa Hospital Universitari Germans Trias i Pujol Universitat Autonoma de Barcelona Badalona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Boesecke","sequence":"additional","affiliation":[{"name":"Bonn University Hospital Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Parera","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3 irsiCaixa Hospital Universitari Germans Trias i Pujol Universitat Autonoma de Barcelona Badalona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Andr\u00e9s","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3 irsiCaixa Hospital Universitari Germans Trias i Pujol Universitat Autonoma de Barcelona Badalona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Franco","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3 irsiCaixa Hospital Universitari Germans Trias i Pujol Universitat Autonoma de Barcelona Badalona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Revollo","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3 Lluita contra la SIDA Hospital Universitari Germans Trias i Pujol Badalona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Ingiliz","sequence":"additional","affiliation":[{"name":"Medical Center for Infectious Diseases (MIB) Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Tural","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3 Lluita contra la SIDA Hospital Universitari Germans Trias i Pujol Badalona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Clotet","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3 irsiCaixa Hospital Universitari Germans Trias i Pujol Universitat Autonoma de Barcelona Badalona Spain"},{"name":"Fundaci\u00f3 Lluita contra la SIDA Hospital Universitari Germans Trias i Pujol Badalona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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