{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:04:25Z","timestamp":1791309865445,"version":"4.3.1"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,1,6]],"date-time":"2016-01-06T00:00:00Z","timestamp":1452038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008990","name":"Universit\u00e9 de Lorraine","doi-asserted-by":"publisher","award":["IN\u2010FR\u2010174\u20100217"],"award-info":[{"award-number":["IN\u2010FR\u2010174\u20100217"]}],"id":[{"id":"10.13039\/100008990","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2016,5]]},"abstract":"Summary<\/jats:title>For hepatitis B virus (HBV<\/jats:styled-content>)\u2010related chronic infection under treatment by nucleos(t)ide analogues (NUC<\/jats:styled-content>s), HB<\/jats:styled-content>sAg clearance is the ultimate therapeutic goal but very infrequent. We investigated how HBV<\/jats:styled-content> envelope protein variability could lead to differential HB<\/jats:styled-content>sAg clearance on NUC<\/jats:styled-content>s. For 12 HBV<\/jats:styled-content> genotype D patients receiving NUC<\/jats:styled-content>s, six resolvers (HB<\/jats:styled-content>sAg clearance) were compared to six matched nonresolvers (HB<\/jats:styled-content>sAg persistence). PreS\/S amino acid (aa) sequences were analysed with bioinformatics to predict HBV<\/jats:styled-content> envelope antigenicity and aa covariance. To enrich our analyses on very rare resolvers, these were compared with other HBV<\/jats:styled-content> genotype D strains in three characterized clinical cohorts including common chronically infected patients. The sT<\/jats:styled-content>125M+sP<\/jats:styled-content>127T combination was observed in four nonresolvers of six, corroborated by aa covariance analysis, associated with a lower predicted antigenicity than sT<\/jats:styled-content>125T+sP<\/jats:styled-content>127P. Concordant features within this HBV<\/jats:styled-content> key functional domain, at positions 125 and 127, were reported from two of the three comparative cohorts. In our hands, a lower ELISA<\/jats:styled-content> reactivity of HBV<\/jats:styled-content>\u2010vaccinated mice sera was observed against the sT<\/jats:styled-content>125M mutant. In the S gene, 56 aa changes in minor variants were detected in non\u2010resolvers, mainly in the major hydrophilic region, vs<\/jats:italic> 28 aa changes in resolvers. Molecular features in patients showing HB<\/jats:styled-content>sAg persistence on NUC<\/jats:styled-content>s argue in favour of a different aa pattern in the HBV<\/jats:styled-content> S gene compared to those showing HB<\/jats:styled-content>sAg clearance. In nonresolvers, a decrease in HB<\/jats:styled-content>s \u2018a\u2019 determinant antigenicity and more frequent mutations in the S gene suggest a role for the HBV<\/jats:styled-content> envelope characteristics in HB<\/jats:styled-content>sAg persistence.<\/jats:p>","DOI":"10.1111\/jvh.12498","type":"journal-article","created":{"date-parts":[[2016,1,8]],"date-time":"2016-01-08T05:40:34Z","timestamp":1452231634000},"page":"387-398","source":"Crossref","is-referenced-by-count":18,"title":["Characterization of hepatitis B virus surface antigen variability and impact on HB<\/scp>s antigen clearance under nucleos(t)ide analogue therapy"],"prefix":"10.1111","volume":"23","author":[{"given":"A.","family":"Velay","sequence":"first","affiliation":[{"name":"EA 7300 \u2018Stress, Immunit\u00e9, Pathog\u00e8nes\u2019 Universit\u00e9 de Lorraine Vandoeuvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Jeulin","sequence":"additional","affiliation":[{"name":"EA 7300 \u2018Stress, Immunit\u00e9, Pathog\u00e8nes\u2019 Universit\u00e9 de Lorraine Vandoeuvre\u2010les\u2010Nancy France"},{"name":"Laboratoire de Virologie Centre Hospitalier Universitaire de Nancy Vandoeuvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Eschlimann","sequence":"additional","affiliation":[{"name":"EA 7300 \u2018Stress, Immunit\u00e9, Pathog\u00e8nes\u2019 Universit\u00e9 de Lorraine Vandoeuvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Malv\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie Centre Hospitalier Universitaire de Nancy Vandoeuvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Goehringer","sequence":"additional","affiliation":[{"name":"Service des Maladies Infectieuses et Tropicales Centre Hospitalier Universitaire de Nancy Vandoeuvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bensenane","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9pato\u2010gastroent\u00e9rologie Centre Hospitalier Universitaire de Nancy Vandoeuvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u2010P.","family":"Frippiat","sequence":"additional","affiliation":[{"name":"EA 7300 \u2018Stress, Immunit\u00e9, Pathog\u00e8nes\u2019 Universit\u00e9 de Lorraine Vandoeuvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Abraham","sequence":"additional","affiliation":[{"name":"Department of Clinical Virology Christian Medical College Vellore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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