{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:45:56Z","timestamp":1792691156010,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,2,19]],"date-time":"2016-02-19T00:00:00Z","timestamp":1455840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004807","name":"California Department of Fish and Game","doi-asserted-by":"publisher","award":["STE 1954\/1\u20101"],"award-info":[{"award-number":["STE 1954\/1\u20101"]}],"id":[{"id":"10.13039\/100004807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002749","name":"Federaal Wetenschapsbeleid","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002749","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Hepatitis E virus (\n                    <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>\n                    ) is the causative agent of hepatitis E in humans and a member of the genus\n                    <jats:italic>Orthohepevirus<\/jats:italic>\n                    in the family\n                    <jats:italic>Hepeviridae<\/jats:italic>\n                    . Infection usually leads to acute hepatitis that can become fulminant, particularly among pregnant women and in patients with preexisting liver disease, or may evolve to a chronic state, especially in immunosuppressed individuals.\n                    <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>\n                    has been shown to produce a range of extra\u2010hepatic manifestations including aplastic anaemia, acute thyroiditis, glomerulonephritis as well as neurological disorders such as Guillain\u2010Barr\u00e9 syndrome, neuralgic amyotrophy and encephalitis. The pathogenesis of these neurological injuries remains largely unknown, and it is also uncertain whether or not\n                    <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>\n                    can directly infect neuronal cells. In this study, we investigated whether\n                    <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>\n                    is capable of completing the viral life cycle in human neuronal\u2010derived cell lines such as neuroepithelioma (\n                    <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>\n                    \u2010N\u2010\n                    <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>\n                    ), desmoplastic cerebellar medulloblastoma (\n                    <jats:styled-content style=\"fixed-case\">DAOY<\/jats:styled-content>\n                    ), glioblastoma multiforme (\n                    <jats:styled-content style=\"fixed-case\">DBTRG<\/jats:styled-content>\n                    ), glioblastoma astrocytoma (U\u2010373\n                    <jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content>\n                    ) and oligodendrocytic (M03.13) cells. Following transfection of these cells with\n                    <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>\n                    <jats:italic>Gaussia<\/jats:italic>\n                    luciferase reporter virus, all tested cell lines supported\n                    <jats:styled-content style=\"fixed-case\">HEV RNA<\/jats:styled-content>\n                    replication. Furthermore, extra\u2010 and intracellular viral capsid was detected by an\n                    <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>\n                    antigen\n                    <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\n                    as a marker for virus assembly and release. Permissiveness for\n                    <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>\n                    cell entry could be demonstrated for the oligodendrocytic cell line M03.13. In conclusion, these results indicate that\n                    <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>\n                    tropism is not restricted to the liver and\n                    <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>\n                    can potentially complete the full viral life cycle in neuronal\u2010derived tissues explaining neurologic disorders during\n                    <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>\n                    infection.\n                  <\/jats:p>","DOI":"10.1111\/jvh.12515","type":"journal-article","created":{"date-parts":[[2016,2,20]],"date-time":"2016-02-20T09:08:35Z","timestamp":1455959315000},"page":"512-521","source":"Crossref","is-referenced-by-count":137,"title":["Extra\u2010hepatic replication and infection of hepatitis E virus in neuronal\u2010derived cells"],"prefix":"10.1111","volume":"23","author":[{"given":"S. A.","family":"Drave","sequence":"first","affiliation":[{"name":"Institute for Experimental Virology TWINCORE Centre for Experimental and Clinical Infection Research A joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI) Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Debing","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Rega Institute for Medical Research, KU Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Walter","sequence":"additional","affiliation":[{"name":"Institute for Experimental Virology TWINCORE Centre for Experimental and Clinical Infection Research A joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI) Hannover 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