{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:00:26Z","timestamp":1761663626832,"version":"3.37.3"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,8,9]],"date-time":"2017-08-09T00:00:00Z","timestamp":1502236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Hepatitis C virus (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>) chronic infection can be associated with extrahepatic manifestations such as mixed cryoglobulinaemia and lymphoproliferative disorders that are endowed with increased rates of morbidity and all\u2010cause mortality. In this study, we used flow cytometry to evaluate the effect of interferon\u2010free antiviral treatment on peripheral blood lymphocytes in <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010infected patients with or without associated lymphoproliferative disorders. Flow cytometry analysis of peripheral blood lymphocytes was performed at baseline and at the end of treatment. In <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010infected patients with lymphoproliferative disorders, we evaluated immunoglobulin (Ig) light chain \u03ba\/\u03bb ratio variations as a measure of monoclonal B\u2010cell response to antiviral therapy. Healthy volunteers were enrolled as controls. A total of 29 patients were included, nine with and 20 without lymphoproliferative disorders. Sustained virological response was achieved in 29 of 29 patients. We observed a significant reduction in the B\u2010cell compartment (39% global reduction) in eight of nine <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010infected patients with lymphoproliferative disorders after viral clearance. We recognized the same trend, even if less pronounced, in <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010infected patients without lymphoproliferative disorders (9% global reduction). Among <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010infected patients with lymphoproliferative disorders, three showed an improvement\/normalization of the immunoglobulin light chain ratio, whereas in the remaining six patients monoclonal B cells persisted to be clonally restricted even 1\u00a0year after the end of treatment. Our data show that <jats:styled-content style=\"fixed-case\">DAA<\/jats:styled-content>s treatment can be effective in reducing the frequency of pathological B cells in the peripheral blood of <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010infected patients affected by <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010associated lymphoproliferative disorders; however, monoclonal populations can persist after viral eradication.<\/jats:p>","DOI":"10.1111\/jvh.12746","type":"journal-article","created":{"date-parts":[[2017,6,23]],"date-time":"2017-06-23T02:26:43Z","timestamp":1498184803000},"page":"1168-1176","source":"Crossref","is-referenced-by-count":21,"title":["Polyclonal and monoclonal B lymphocytes response in <scp>HCV<\/scp>\u2010infected patients treated with direct\u2010acting antiviral agents"],"prefix":"10.1111","volume":"24","author":[{"given":"A.","family":"Schiavinato","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine University\u2010Hospital of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Zanetto","sequence":"additional","affiliation":[{"name":"Multivisceral Transplant Unit Gastroenterology Department of Surgery, Oncology and Gastroenterology Padua University Hospital  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Pantano","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University\u2010Hospital of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Tosato","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University\u2010Hospital of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Nabergoj","sequence":"additional","affiliation":[{"name":"Department of Hematology and Clinical Immunology University\u2010Hospital of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Fogar","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University\u2010Hospital of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Piva","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University\u2010Hospital of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Gambato","sequence":"additional","affiliation":[{"name":"Multivisceral Transplant Unit Gastroenterology Department of Surgery, Oncology and Gastroenterology Padua University Hospital  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Franceschet","sequence":"additional","affiliation":[{"name":"Multivisceral Transplant Unit Gastroenterology Department of Surgery, Oncology and Gastroenterology Padua University Hospital  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Floreani","sequence":"additional","affiliation":[{"name":"Multivisceral Transplant Unit Gastroenterology Department of Surgery, Oncology and Gastroenterology Padua University Hospital  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Farinati","sequence":"additional","affiliation":[{"name":"Multivisceral Transplant Unit Gastroenterology Department of Surgery, Oncology and Gastroenterology Padua University Hospital  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Burra","sequence":"additional","affiliation":[{"name":"Multivisceral Transplant Unit Gastroenterology Department of Surgery, Oncology and Gastroenterology Padua University Hospital  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4127-8941","authenticated-orcid":false,"given":"F. 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