{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T15:45:30Z","timestamp":1762875930970},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,12,19]],"date-time":"2017-12-19T00:00:00Z","timestamp":1513641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470852"],"award-info":[{"award-number":["81470852"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We previously identified a novel inhibitor of La protein, H11, which inhibited hepatitis B virus (<jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>) replication by inhibiting the interaction between La protein and <jats:styled-content style=\"fixed-case\">HBV RNA<\/jats:styled-content>. However, the other cellular factors involved in this process remain unclear. To investigate the mechanism of H11\u2010mediated inhibition of <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> infection, a lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> microarray analysis was performed using H11\u2010treated and untreated stable <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>\u2010expressing human hepatoblastoma HepG2.2.15 cells. The profiles of differentially expressed lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s were generated and analysed using Gene Ontology (<jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content>) and pathway analyses. The microarray data showed that 61 lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were upregulated, 74 lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were downregulated, 43 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s were upregulated, and 44 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s were downregulated in H11 treatment group when compared with the control group, and these results were consistent with <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> expression data. Bioinformatic analysis indicated that the differentially expressed lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were involved in <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010mediated post\u2010transcriptional gene silencing, regulation of viral genome replication and Jak\u2010<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content> signalling and apoptosis pathways. <jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content> analysis showed that differentially expressed <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s were enriched in negative regulation of the Wnt signalling pathway and negative regulation of growth. Pathways analysis indicated that the differentially expressed <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s were involved in regulation of nuclear \u03b2\u2010catenin signalling and target gene transcription, as direct p53 effectors, and in the peroxisome proliferator\u2010activated receptors signalling and peroxisome pathways. Microarray data and <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> results indicated that H11 mediates inhibition of <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> replication by regulating the Wnt, \u03b2\u2010catenin and <jats:styled-content style=\"fixed-case\">PPAR<\/jats:styled-content> signalling pathways.<\/jats:p>","DOI":"10.1111\/jvh.12821","type":"journal-article","created":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T14:52:37Z","timestamp":1509547957000},"page":"391-400","source":"Crossref","is-referenced-by-count":7,"title":["Lnc<scp>RNA<\/scp> expression profiles in <scp>HBV<\/scp>\u2010transformed human hepatocellular carcinoma cells treated with a novel inhibitor of human La protein"],"prefix":"10.1111","volume":"25","author":[{"given":"J.","family":"Pan","sequence":"first","affiliation":[{"name":"Department of Pharmacy The Obstetrics &amp; Gynecology Hospital of Fudan University  Shanghai China"},{"name":"Department of Clinical Pharmacy Shanghai General Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy Shanghai General Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The Obstetrics &amp; Gynecology Hospital of Fudan University  Shanghai China"},{"name":"Department of Clinical Pharmacy Shanghai General Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2014-308943"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60220-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2016.7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11908-016-0527-8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-015-0682-y"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.10.027"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201911200"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00429-5"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2741\/3097"},{"key":"e_1_2_8_11_1","first-page":"735","article-title":"[A study on the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein]","volume":"14","author":"Zhang H","year":"2006","journal-title":"Zhonghua Gan Zang Bing Za Zhi"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i14.2050"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2893.2012.01636.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036363"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2014.07.005"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2015.68"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.10.021"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2015.07.025"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.05.014"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-016-2174-5"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3606"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.02.006"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-11-59"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj021"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.146"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01824-16"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-014-2951-4"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22200"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1502677"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep24744"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.048744.114"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01562-12"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/145124"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2016.01.025"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i20.6317"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/330667a0"}],"container-title":["Journal of Viral Hepatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjvh.12821","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jvh.12821","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T08:02:35Z","timestamp":1695542555000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jvh.12821"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,19]]},"references-count":35,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/jvh.12821"],"URL":"https:\/\/doi.org\/10.1111\/jvh.12821","archive":["Portico"],"relation":{},"ISSN":["1352-0504","1365-2893"],"issn-type":[{"value":"1352-0504","type":"print"},{"value":"1365-2893","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,19]]}}}