{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T13:09:57Z","timestamp":1759410597454,"version":"3.37.3"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T00:00:00Z","timestamp":1519171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000046","name":"National Research Council Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Hepatitis C virus (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>) chronically infects 2%\u20103% of the world's population, causing liver disease and cancer with prolonged infection. The narrow host range of the virus, being restricted largely to human hepatocytes, has made the development of relevant models to evaluate the efficacy of vaccines a challenge. We have developed a novel approach to accomplish this by generating a murine hepatoma cell line stably expressing nonstructural <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> antigens which can be used in vitro or in vivo to test <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> vaccine efficacies. These <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010recombinant hepatoma cells formed large solid\u2010mass tumours when implanted into syngeneic mice, allowing us to test candidate <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> vaccines to demonstrate the development of an <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010specific immune response that limited tumour growth. Using this model, we tested the therapeutic potential of recombinant anti\u2010<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010specific vaccines based on two fundamentally different attenuated pathogen vaccine systems\u2014attenuated <jats:italic>Salmonella<\/jats:italic> and recombinant adenoviral vector based vaccine. While attenuated <jats:italic>Salmonella<\/jats:italic> that secreted <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> antigens limited growth of the <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010recombinant tumours when used in a therapeutic vaccination trial, replication\u2010competent but noninfectious adenovirus expressing nonstructural HCV antigens showed overall greater survival and reduced weight loss compared to non\u2010replicating nondisseminating adenovirus. Our results demonstrate a model with anti\u2010tumour responses to <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> nonstructural (<jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>) protein antigens and suggest that recombinant vaccine vectors should be explored as a therapeutic strategy for controlling <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010associated cancers.<\/jats:p>","DOI":"10.1111\/jvh.12856","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T04:01:21Z","timestamp":1515556881000},"page":"649-660","source":"Crossref","is-referenced-by-count":5,"title":["Development of a recombinant murine tumour model using hepatoma cells expressing hepatitis C virus nonstructural antigens"],"prefix":"10.1111","volume":"25","author":[{"given":"K. 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