{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T20:50:57Z","timestamp":1789159857568,"version":"build-2803163510"},"reference-count":9,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,22]],"date-time":"2020-02-22T00:00:00Z","timestamp":1582329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diagnostic of acute hepatitis A virus (HAV) infection is based on the detection of anti\u2010HAV IgM without testing for the pathogen itself. We evaluated the usefulness of HAV RNA testing for confirmation of acute hepatitis A and to provide indications about the level of HAV replication in HIV\u2010positive and HIV\u2010negative subjects during an unprecedented outbreak of HAV observed in France in 2017. HAV RNA was detected in 38 out of 41 (92.6%) subjects with a clinical diagnosis of acute hepatitis A, whereas nine cases tested positive for anti\u2010HAV IgM in whom the diagnosis of acute hepatitis A was not retained were found negative for HAV RNA. All subjects in the control group were also tested negative for HAV RNA. HAV viremia was correlated to ALT peak (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.64; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001). HIV\u2010infected patients have similar HAV RNA levels but were less likely to have prolonged international normalized ratio of prothrombin time when compared to the HIV\u2010uninfected group (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.016), suggesting a less severe course of acute hepatitis. HAV RNA was detected in the serum of most of the patients with acute hepatitis A, indicating that the direct detection of HAV can be used to confirm hepatitis A in patients tested positive for anti\u2010HAV IgM antibodies. Nucleic acid tests should serve more broadly during the diagnosis workup of acute hepatitis A to improve the predictive values of HAV in vitro diagnostic tests and to confirm acute hepatitis A in patients tested positive with IgM with moderate or low S\/CO values.<\/jats:p>","DOI":"10.1111\/jvh.13255","type":"journal-article","created":{"date-parts":[[2020,1,2]],"date-time":"2020-01-02T16:04:48Z","timestamp":1577981088000},"page":"540-543","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["RNA testing for the diagnosis of acute hepatitis A during the 2017 outbreak in France"],"prefix":"10.1111","volume":"27","author":[{"given":"Julie","family":"Tram","sequence":"first","affiliation":[{"name":"Pathogenesis and Control of Chronic Infections INSERM Etablissement fran\u00e7ais du Sang CHU Montpellier University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Le Baccon\u2010Sollier","sequence":"additional","affiliation":[{"name":"Pathogenesis and Control of Chronic Infections INSERM Etablissement fran\u00e7ais du Sang CHU Montpellier University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2142-0755","authenticated-orcid":false,"given":"Karine","family":"Bollor\u00e9","sequence":"additional","affiliation":[{"name":"Pathogenesis and Control of Chronic Infections INSERM Etablissement fran\u00e7ais du Sang CHU Montpellier University of Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Ducos","sequence":"additional","affiliation":[{"name":"CHU Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Marie","family":"Mondain","sequence":"additional","affiliation":[{"name":"CHU Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Pastor","sequence":"additional","affiliation":[{"name":"CHU Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges\u2010Philippe","family":"Pageaux","sequence":"additional","affiliation":[{"name":"Hepatology and Liver Transplantation Unit CHU Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Makinson","sequence":"additional","affiliation":[{"name":"INSERM U1175\/IRD UMI 233. 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