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However, in resource\u2010limited countries, identification of patients with active HCV infection is still challenging in remote settings due to the limited access to laboratories able to measure HCV viral load. This study evaluated whether dried blood spots (DBS) transferred to a central laboratory could overcome this challenge. A total of 315 HCV\u2010infected patients, na\u00efve to anti\u2010HCV treatment, provided each three type of samples: plasma, DBS with calibrated quantities of venous blood and DBS with uncalibrated quantities of capillary blood. Qualitative comparison was conducted in terms of detection of HCV viral load on DBS as opposed to plasma to estimate sensitivity and specificity. Quantitative comparisons were conducted by means of correlation estimation. Of the 250 patients with detected plasma HCV viral load, 245 also had detectable DBS HCV viral load (capillary or venous) leading to a sensitivity of 98.0% (95% confidence interval (CI): 95.4%\u201099.3%); importantly, all measurements with a plasma HCV viral load &gt;118\u00a0IU\/mL were also detected in DBS. When HCV was not detected in plasma, it was also not detected in DBS resulting in 100% specificity (95% CI: 94.5%\u2010100%). Quantitative HCV viral load results were very similar when utilizing plasma or DBS sample types as illustrated by correlations &gt;0.99. In conclusion, DBS sample types, with either uncalibrated capillary blood or calibrated venous blood, performed well to distinguish patients with active HCV infection, and who therefore need treatment, from other patients.<\/jats:p>","DOI":"10.1111\/jvh.13263","type":"journal-article","created":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T04:29:16Z","timestamp":1579926556000},"page":"514-519","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Dried blood spots perform well to identify patients with active HCV infection in Vietnam"],"prefix":"10.1111","volume":"27","author":[{"given":"Tram Hong","family":"Tran","sequence":"first","affiliation":[{"name":"National Reference Laboratory of HIV Molecular Biology National Institute of Hygiene and Epidemiology Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binh Thanh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"National Reference Laboratory of HIV Molecular Biology National Institute of Hygiene and Epidemiology Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuan Anh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"National Reference Laboratory of HIV Molecular Biology National Institute of Hygiene and Epidemiology Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tram Thi Phuong","family":"Pham","sequence":"additional","affiliation":[{"name":"National Reference Laboratory of HIV Molecular Biology National Institute of Hygiene and Epidemiology Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trang Thi Thu","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Nam Tu Liem District Medical Center Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong Thi Bich","family":"Mai","sequence":"additional","affiliation":[{"name":"Nam Tu Liem District Medical Center Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hien Ba","family":"Pham","sequence":"additional","affiliation":[{"name":"Dong Da General Hospital Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thai Minh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Dong Da General Hospital Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huong Thi Thu","family":"Phan","sequence":"additional","affiliation":[{"name":"Vietnam Administration of HIV\/AIDS Control Ministry of Health Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nhan Thi","family":"Do","sequence":"additional","affiliation":[{"name":"Vietnam Administration of HIV\/AIDS Control Ministry of Health Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohand","family":"Ait\u2010Ahmed","sequence":"additional","affiliation":[{"name":"Center for Translational Research Institut Pasteur Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabien","family":"Taieb","sequence":"additional","affiliation":[{"name":"Center for Translational Research Institut Pasteur Paris France"},{"name":"Emerging Diseases Epidemiology Unit Institut Pasteur Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6201-1261","authenticated-orcid":false,"given":"Yoann","family":"Madec","sequence":"additional","affiliation":[{"name":"Emerging Diseases Epidemiology Unit Institut Pasteur Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,11]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Organization WH.Global hepatitis report 2017(available at:https:\/\/www.who.int\/hepatitis\/publications\/global-hepatitis-report2017\/en\/; 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