{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:43:38Z","timestamp":1790790218093,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T00:00:00Z","timestamp":1584403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000404","name":"Foundation for Liver Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000404","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007013","name":"F. Hoffmann-La Roche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007013","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis B virus (HBV) RNA in serum is a novel biomarker that reflects cccDNA activity. We investigated whether HBV RNA can predict serological response to peginterferon (PEG\u2010IFN) treatment. Serum HBV RNA levels were retrospectively measured at weeks 0, 12, 24 and 52 of therapy and after treatment discontinuation (week 78) in 266 HBeAg\u2010positive chronic HBV patients who had participated in a global randomized controlled trial (HBV99\u201001 study). Patients received 52\u00a0weeks PEG\u2010IFN monotherapy (n\u00a0=\u00a0136) or PEG\u2010IFN and lamivudine (n\u00a0=\u00a0130). The primary end point was HBeAg loss 24\u00a0weeks after PEG\u2010IFN discontinuation. At baseline, the mean serum level of HBV RNA was 6.8 (SD 1.2) log c\/mL. HBV RNA levels declined to 4.7 (1.7) log c\/mL after one year of PEG\u2010IFN therapy alone and to 3.3 (1.2)log c\/mL after combination therapy. From week 12 onward, HBV RNA level was significantly lower in patients who achieved HBeAg loss at the end of follow\u2010up as compared to those who did not, regardless of treatment allocation (week 12:4.4 vs 5.1 log c\/mL, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.01; week 24:3.7 vs 4.9 log c\/mL, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001). The performance of a multivariable model based on HBV RNA level was comparable at week 12 (AUC 0.68) and 24 (AUC 0.72) of therapy. HBV RNA level above 5.5 log c\/mL at week 12 showed negative predictive values of 93\/67\/90\/64% for HBV genotypes A\/B\/C\/D for the prediction of HBeAg loss. In conclusion, HBV RNA in serum declines profoundly during PEG\u2010IFN treatment. Early on\u2010treatment HBV RNA level may be used to predict nonresponse.<\/jats:p>","DOI":"10.1111\/jvh.13272","type":"journal-article","created":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T06:51:46Z","timestamp":1581576706000},"page":"610-619","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Serum hepatitis B virus RNA predicts response to peginterferon treatment in HBeAg\u2010positive chronic hepatitis B"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0460-4920","authenticated-orcid":false,"given":"Margo J. H.","family":"van Campenhout","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology Erasmus MC University Medical Center Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"van B\u00f6mmel","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Section of Hepatology University Hospital Leipzig Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Pfefferkorn","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Section of Hepatology University Hospital Leipzig Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janett","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Section of Hepatology University Hospital Leipzig Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo","family":"Deichsel","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Section of Hepatology University Hospital Leipzig Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8607-1616","authenticated-orcid":false,"given":"Andr\u00e9","family":"Boonstra","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Erasmus MC University Medical Center Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anneke J.","family":"van Vuuren","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Erasmus MC University Medical Center Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Berg","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Section of Hepatology University Hospital Leipzig Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina E.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Erasmus MC University Medical Center Rotterdam The Netherlands"},{"name":"Institute of Health Policy, Management and Evaluation University of Toronto Toronto Canada"},{"name":"Toronto Center for Liver Disease Toronto Western and General Hospital University Health Network Toronto Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry L. A.","family":"Janssen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Erasmus MC University Medical Center Rotterdam The Netherlands"},{"name":"Toronto Center for Liver Disease Toronto Western and General Hospital University Health Network Toronto Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,17]]},"reference":[{"key":"e_1_2_9_3_1","unstructured":"World Health Organization.Hepatitis B. 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