{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:19:12Z","timestamp":1758629952720,"version":"3.40.5"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T00:00:00Z","timestamp":1607126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>HCV is key pathological factor for inducting insulin resistance. Such HCV\u2010induced insulin resistance is linked with metabolic syndrome, type 2 diabetes mellitus, extrahepatic manifestations, hepatic fibrosis progression and development of hepatocellular carcinoma. DNA methylation alterations can cause developmental abnormalities, tumours and other diseases. In our study, PBMCs were isolated and genomic DNA was extracted. DNA fragmentation was achieved by sonication to 200\u2010400\u00a0bp; subsequently, end repair and adenylation was performed. Manufacturer's guidelines were followed to ligate Cytosine\u2010methylated barcodes to sonicated DNA. EZ DNA Methylation\u2010GoldTM Kit was then employed to treat these DNA segments twice with bisulphite. A Library was maintained, sequenced on an Illumina platform and 150\/125\u00a0bp paired\u2010end reads generated. GO seq R package was used to perform Gene Ontology (GO) enrichment analysis for genes linked to DMRs and DMPs; gene length bias was corrected. We identified 12\u00a0945 significant hypermethylated DMRs among all samples that were screened as those with at least 0.1 methylation level differences and <jats:italic>P<\/jats:italic>\u2010value less than 0.05. Fisher's exact test with FDR multiple test correction is used for identification of DMPs and DMRs. High throughput bisulphite sequencing (Illumina) was carried out, and bioinformatics analysis was performed to analyse methylation status. Gene ontology (GO) and KEGG pathway enrichment analysis showed differentially methylated regions enriched in various pathways that include PI3K\u2010AKT\/IRS1 signalling pathway, metabolic pathway, oxidative phosphorylation, Renin\u2010angiotensin system that are all involved in developing type\u20102 diabetes (T2D). Our study provides supporting evidence for significant involvement of HCV infection in development of epigenetic modifications in regulation of metabolic disorders like T2D and its complications.<\/jats:p>","DOI":"10.1111\/jvh.13417","type":"journal-article","created":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T06:41:01Z","timestamp":1602657661000},"page":"245-259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genome\u2010wide methylation profiling of HCV pathogenesis to develop diabetes and diabetic complications"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0575-8246","authenticated-orcid":false,"given":"Shazia","family":"Rafique","sequence":"first","affiliation":[{"name":"Division of Molecular Virology Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadia","family":"Zahid","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amjad","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering Hazara, University Mansehra Khyber Pakhtunkhwa Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Tariq","sequence":"additional","affiliation":[{"name":"Department of Biology Lahore University of Management Sciences Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maham","family":"Saeed","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kashif","family":"Iqbal Sahibzada","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biotechnology (IBB) University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Ali Shahid","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Idrees","sequence":"additional","affiliation":[{"name":"Division of Molecular Virology Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,5]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"469","article-title":"Epigenetics in the development of diabetic cardiomyopathy","volume":"11","author":"Pepin ME","year":"2019","journal-title":"Future Med"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.228262"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42602-2"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cld.694"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2015.00134"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.02.006"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.947102"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063852"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063443"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00329"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-38980-2"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-38980-2"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-018-0241-6"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2337\/db18-0290"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr167"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm882"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti430"},{"key":"e_1_2_9_20_1","doi-asserted-by":"crossref","unstructured":"LiuX.Research progresses of the mechanism of insulin resistance in type II diabetes. 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