{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:40:33Z","timestamp":1747186833121,"version":"3.40.5"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871230","81401298"],"award-info":[{"award-number":["81871230","81401298"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to clarify the expression of HLA\u2010DQ and granulysin in peripheral blood T\u2010cell subsets in patients with chronic hepatitis B virus (CHB) and to evaluate their significance in assisting CHB diagnosis and immune status assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral blood from 34 CHB patients, 36 inactive HBsAg carriers and 33\u00a0healthy controls were collected, and HLA\u2010DQ and granulysin in a series of T\u2010cell subsets were analysed by flow cytometry. The ability to secrete IL\u201010 and IFN\u2010\u03b3 and the functional T\u2010cell subsets were measured in Treg and CD4 cells expressing HLA\u2010DQ or not. Correlation analyses were further conducted between HLA\u2010DQ\/granulysin\u2010related subsets and clinical indicators of HBV infection, and ROC curves were built to evaluate diagnosis efficiency of HLA\u2010DQ\u2010related subsets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HLA\u2010DQ+ percentages in circulating CD4\u00a0T cells were downregulated in CHB patients. The proportions of HLA\u2010DQ\u00a0+\u00a0Tfh in CHB were upregulated while HLA\u2010DQ+ percentages in Treg were decreased. In terms of function, the IFN\u2010\u03b3 secretion ability of CD4\u00a0+\u00a0T cells and IL\u201010\u00a0secretion in Tregs were stronger in HLA\u2010DQ+ than HLA\u2010DQ\u2010 subsets. HLA\u2010DQ\u00a0+\u00a0CD4\u00a0+\u00a0T cells and HLA\u2010DQ\u00a0+\u00a0Treg were negatively correlated with HBV\u2010DNA, while HLA\u2010DQ\u00a0+\u00a0Tfh and Tfc cells were positively correlated with HBV\u2010DNA and ALT. HLA\u2010DQ\u00a0+\u00a0Treg\/Tfh\/Tfc could help to distinguish CHB from inactive HBsAg carriers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HLA\u2010DQ on T cells can characterize the function of T\u2010cell subsets and analysis of HLA\u2010DQ can help to evaluate immune status and assist in diagnosis of CHB.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jvh.13669","type":"journal-article","created":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T02:19:35Z","timestamp":1646965175000},"page":"340-351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The altered HLA\u2010DQ expression in peripheral blood T cells of chronic hepatitis B patients characterizes the function of T cells"],"prefix":"10.1111","volume":"29","author":[{"given":"Xingyue","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Peking University People\u2019s Hospital  Beijing 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