{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T04:21:36Z","timestamp":1792815696393,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005564","name":"Gilead Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>HBV RNA is considered as a promising predictor in patients who discontinue nucleos(t)ide analogues (NAs). We determined HBV RNA levels in non\u2010cirrhotic HBeAg\u2010negative patients who discontinued NAs and assessed their predictability for 12\u2010month outcomes. Fifty\u2010seven patients of DARING\u2010B study were included. HBV RNA levels were determined in stored monthly serum samples drawn at 0\u20133\u00a0months after end of therapy (EOT). Other markers previously determined in the same cohort including hepatitis B core\u2010related antigen (HBcrAg) were also assessed. HBV RNA at EOT was detectable in 7% of patients, who developed virological\/clinical relapse and required retreatment at month 2; in patients with undetectable EOT HBV RNA, 12\u2010month cumulative rates of virological relapse, clinical relapse and retreatment were 68%, 28% and 21%, respectively (<jats:italic>p<\/jats:italic>\u2009\u2264\u20090.008). HBV RNA at month\u20101 after EOT was detectable in 19% of patients being associated with higher probability only of virological relapse (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.001). HBV RNA levels correlated significantly to HBV DNA, HBcrAg, ALT and interferon\u2010induced protein\u201010, but not HBsAg levels. Combined EOT HBV RNA and HBcrAg detection and\/or HBsAg &gt;1000\u2009IU\/ml was associated only with higher probability of retreatment having higher sensitivity and lower specificity than HBV RNA alone. In conclusion, serum HBV RNA is detectable in a minority of non\u2010cirrhotic HBeAg\u2010negative patients under effective long\u2010term NAs therapy offering low sensitivity but 100% specificity for early retreatment due to severe clinical relapses after NA discontinuation. The combinations of EOT HBV RNA with HBcrAg and\/or high HBsAg levels increase sensitivity but decrease specificity for prediction of retreatment after NAs withdrawal.<\/jats:p>","DOI":"10.1111\/jvh.13729","type":"journal-article","created":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T14:14:43Z","timestamp":1657030483000},"page":"948-957","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Significance of serum <scp>HBV RNA<\/scp> in non\u2010cirrhotic <scp>HBeAg<\/scp>\u2010negative chronic hepatitis B patients who discontinue effective antiviral therapy"],"prefix":"10.1111","volume":"29","author":[{"given":"Margarita","family":"Papatheodoridi","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Medical School of National and Kapodistrian University of Athens Laiko General Hospital  Athens Greece"},{"name":"Institute of Liver and Digestive Health University College of London  London 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