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Efficient and scientific detection methods are important for clinical monitoring of cccDNA and targeted drug development. Western blotting is the gold standard for the quantitative detection of cccDNA, but it is time\u2010consuming and complex. In recent years, new detection technologies have been continuously updated. There are new developments and breakthroughs in both next\u2010generation polymerase chain reaction (PCR) and non\u2010PCR methods such as in situ hybridization. Some HBV\u2010related markers (such as hepatitis B\u00a0core\u2010related\u00a0antigen) have also been shown to be closely related to cccDNA, and they can be used as surrogate markers to indirectly reflect cccDNA content. In this paper, the main detection methods of cccDNA and their improvements are reviewed, the advantages and limitations of these methods are analysed and summarized, and future development directions are proposed.<\/jats:p>","DOI":"10.1111\/jvh.13817","type":"journal-article","created":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T08:18:19Z","timestamp":1675844299000},"page":"366-373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Research progress on detection methods for hepatitis B virus covalently closed circular <scp>DNA<\/scp>"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7751-7735","authenticated-orcid":false,"given":"Fenglan","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Jingzhou Hospital Affiliated to Yangtze University  Jingzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7198-0825","authenticated-orcid":false,"given":"Wei","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Jingzhou Hospital Affiliated to Yangtze University  Jingzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1575-2902","authenticated-orcid":false,"given":"Yaoling","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Jingzhou Hospital Affiliated to Yangtze University  Jingzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,2,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_7_3_1","unstructured":"Hepatitis B[EB\/OL].2022. 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