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Aim of the present study is to describe the evolution of inflammatory markers used in clinical practice, mixed cryoglobulinemia (MC) and autoantibody reactivity in co\u2010infected individuals who achieved sustained virological response (SVR) after DAA treatment. This prospective, observational study included all HIV\/HCV co\u2010infected subjects who started any DAA regimen from 2015 to 2020. Samples for laboratory measurements (ferritin, C reactive protein, C3 and C4 fractions, rheumatoid factor, MC, anti\u2010thyroglobulin Ab, anti\u2010thyroid peroxidase Ab, ANCA, ASMA, anti\u2010LKM, anti\u2010DNA, AMA, ANA, T CD4+ and CD8+ cell count, and CD4\/CD8 ratio) were collected at baseline, after 4\u2009weeks, at end of treatment, and at SVR12. The analysis included 129 individuals: 51.9% with a F0\u2013F3 fibrosis and 48.1% with liver cirrhosis. Cryocrit, C3 fraction, and rheumatoid factor significantly improved at week 4; ferritin, anti\u2010thyroglobulin Ab, and C4 fraction at EOT; total leukocytes count at SVR12. MC positivity decreased from 72.8% to 35.8% (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). T CD4+ cell slightly increased at SVR12, but with an increase also in CD8+ resulting in stable CD4\/CD8 ratio. Autoantibody reactivity did not change significantly. ANA rods and rings positivity increased from 14.8% to 28.6% (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.099): they were observed in three subjects without exposure to RBV. DAA therapy may lead to improvement in inflammatory markers and MC clearance but without significant changes in autoantibodies reactivity and CD4\/CD8 ratio over a follow up of 12\u2009weeks.<\/jats:p>","DOI":"10.1111\/jvh.13818","type":"journal-article","created":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T21:30:00Z","timestamp":1676151000000},"page":"530-539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Impact of treatment with direct\u2010acting antivirals on inflammatory markers and autoantibodies in HIV\/HCV co\u2010infected individuals"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4965-8789","authenticated-orcid":false,"given":"Roberto","family":"Rossotti","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases ASST Grande Ospedale Metropolitano Niguarda Milan 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