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This systematic review evaluated the performance of dried blood spots (DBS) as a collection and storage tool for diagnosing an hepatitis B virus (HBV) infection. A comprehensive search using OVID, Scopus and CINAHL databases was performed to collate articles published up to April 2023 that detected Hepatitis B infections using DBS. Five reviewers independently performed identification, screening, quality assessment and data extraction. A qualitative synthesis of the included studies was conducted. Of the 402 articles, 78 met the inclusion criteria. The results show that most studies focused on populations with known HBV, HCV and\/or HIV status. Approximately half (49%) of the included studies utilized the Whatman Protein Saver Card for DBS collection. The DBS samples were then predominantly stored in room temperature conditions. In line with this, storage conditions influenced the concentration and stability of the analyte from the DBS samples, affecting the accuracy of downstream diagnostic methods. ELISA methods, using hepatitis B surface antigen (HBsAg) as an HBV marker, were the most widely used diagnostic tool for detecting HBV infection in DBS samples. The simplicity and cost\u2010effectiveness of the ELISA technique highlight its potential to be used in low\u2010resource settings. In line with this, the detection of HBsAg using an ELISA immunoassay had higher sensitivity (85.6%\u2013100%), and specificity (95%\u2013100%) ranges as compared to other target molecules and methods. Although this review only performed a qualitative analysis, DBS offers a promising method for collecting and storing blood samples; however, the standardization of sampling, storing conditions and diagnostic techniques is required to ensure sustainable application.<\/jats:p>","DOI":"10.1111\/jvh.13890","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T09:20:26Z","timestamp":1696411226000},"page":"35-46","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The role of dried blood spot tests in the detection of hepatitis B infection: A systematic review"],"prefix":"10.1111","volume":"31","author":[{"given":"Maria Concepcion C.","family":"Virtudazo","sequence":"first","affiliation":[{"name":"Department of Biology, College of Arts and Sciences University of the Philippines Manila  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jericho B.","family":"Aquino","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Arts and Sciences University of the Philippines Manila  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rose Nicole B.","family":"Arellano","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Arts and Sciences University of the Philippines Manila  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Fortes","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Arts and Sciences University of the Philippines Manila  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphaela C.","family":"Kaw","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Arts and Sciences University of the Philippines Manila  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ourlad Alzeus G.","family":"Tantengco","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Arts and Sciences University of the Philippines Manila  Manila Philippines"},{"name":"Department of Physiology, College of Medicine University of the Philippines Manila  Manila Philippines"},{"name":"Department of Biology, College of Science De La Salle University  Manila Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,3]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22881"},{"key":"e_1_2_12_3_1","unstructured":"TripathiN MousaO.Hepatitis. 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