{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:59:36Z","timestamp":1760162376894,"version":"3.41.2"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T00:00:00Z","timestamp":1732233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>An HCV elimination program aims to diagnose more than 90% of Chronic HCV cases. We critically evaluated the performance of a point\u2010of\u2010care test (POCT) using an HCV Rapid Antibody Test. PWID from 4 Halfway Houses (HWH) in Singapore were recruited from March 2022 to April 2023. Participants were concurrently screened for HCV via venous blood for anti\u2010HCV serology and using fingerstick capillary whole blood (FSWB) and oral mucosal transudates (OMT) for POCT, which were interpreted by trained personnel. A blinded study team member independently assessed images of POCTs. Of 207 participants, 37.3% were anti\u2010HCV positive. Compared to anti\u2010HCV serology, POCT performance on FSWB and OMT were: Sensitivity 81.8 (73.2\u201390.4), 74.0 (64.2\u201383.8), <jats:italic>p<\/jats:italic>\u2009=\u20090.014; Specificity 100.0 (100.0\u2013100.0), 98.5 (96.3\u2013100), <jats:italic>p<\/jats:italic>\u2009=\u20090.157. Sub\u2010group analysis of strict 30\u2010min pre\u2010test nil\u2010by\u2010mouth instruction in 103 subjects reported Sensitivity 77.5 (64.6\u201390.4), 77.5 (64.6\u201390.4) and Specificity 100.0 (100.0\u2013100.0) and 98.4 (95.3\u2013100.0). OMT positivity and false\u2010negative outcomes did not correlate with the sample analytical cutoff index signal distribution of anti\u2010HCV Serology. Inter\u2010class correlation between real\u2010time and imaging readings of POCT for FSWB and OMT at 20 and 40\u2009min were Kappa 0.9666, 0.9674; 0.8803, 0.8940. Proper preparation and patient selection enhance test performance. Differences in oral fluid immunoglobulin secretion, oral pathology, age, and sample collection can affect POCT OMT readings. POCT OMT is promising in serial and self\u2010testing, complementing its convenience in testing.<\/jats:p>","DOI":"10.1111\/jvh.14039","type":"journal-article","created":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T11:29:49Z","timestamp":1732274989000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Real\u2010World Evaluation of Point\u2010of\u2010Care Testing for Hepatitis C Antibodies: Navigating Hepatitis C Elimination Effort"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2005-675X","authenticated-orcid":false,"given":"Kuo Chao","family":"Yew","sequence":"first","affiliation":[{"name":"Department of Gastroenterology &amp; Hepatology Tan Tock Seng Hospital  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssa Shin Yee","family":"Sim","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology &amp; Hepatology Tan Tock Seng Hospital  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Hawkins","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Tan Tock Seng Hospital  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanchalika","family":"Acharyya","sequence":"additional","affiliation":[{"name":"Clinical Research &amp; Innovation Office Tan Tock Seng Hospital  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerard Daquan","family":"Wong","sequence":"additional","affiliation":[{"name":"HCSA Highpoint Halfway House HCSA Community Services  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel Wei Chong","family":"Wong","sequence":"additional","affiliation":[{"name":"HCSA Highpoint Halfway House HCSA Community Services  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan Heng","family":"Lim","sequence":"additional","affiliation":[{"name":"Lee Kong Chian School of Medicine Nanyang Technological University  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei Lyn","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology &amp; Hepatology Tan Tock Seng Hospital  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Yen","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Preventive and Population Medicine Tan Tock Seng Hospital  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela Li Ping","family":"Chow","sequence":"additional","affiliation":[{"name":"Lee Kong Chian School of Medicine Nanyang Technological University  Singapore Singapore"},{"name":"Department of Preventive and Population Medicine Tan Tock Seng Hospital  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,22]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_11_2_1","DOI":"10.1111\/jvh.12668"},{"volume-title":"Guidance for Country Validation of Viral Hepatitis Elimination and Path to Elimination","year":"2023","author":"World Health Organization","key":"e_1_2_11_3_1"},{"volume-title":"Making Diagnosis of Hepatitis C More Accessible and Closer to the Community Using Point\u2010of\u2010Care HCV Vrial Load Assays","year":"2023","author":"New WHO Study","key":"e_1_2_11_4_1"},{"volume-title":"Hepatitis C","year":"2024","author":"World Health 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