{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T19:14:43Z","timestamp":1788462883093,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T00:00:00Z","timestamp":1740355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100014857","name":"National Natural Science Foundation of China-Guangdong Joint Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014857","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Hepatocellular carcinoma (HCC) and liver cirrhosis (LC) occur in spite of current antiviral therapies in patients with chronic hepatitis (CHB). It is not yet known why HCC and LC are related to hepatocytes and hepatic stellate cells (HSCs), respectively, in the same inflammation circumstances. The expression of the phosphorylated form of histone H2AX (\u03b3\u2010H2AX), a biomarker of DNA damage, was detected in hepatocytes and interstitial cells within the liver tissues of 69 patients with CHB using immunohistochemical assay and immunofluorescence colocalisation technique. Hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) was applied to establish an oxidative DNA damage model. Hepatocytes in CHB patients carried much higher levels of DNA damage than interstitial cells. The DNA damage\u2010carried interstitial cells were confirmed to be HSCs. They lost the damaged DNA during differentiation into myofibroblasts near the foci of inflammatory necrosis. Hepatocyte was much more sensitive to oxidative stress and DNA damage than HSCs, but both MIHA and LX\u20102 repaired DNA damage efficiently in\u00a0vitro. Hepatocytes carried much higher levels of DNA damage than HSCs due to their remarkable difference in sensitivity to inflammation\u2010induced oxidative DNA damage. The different sensitivity may render hepatocytes and HSCs to be respectively involved in HCC and LC in the same inflammation circumstances.<\/jats:p>","DOI":"10.1111\/jvh.70017","type":"journal-article","created":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T04:41:45Z","timestamp":1740544905000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Hepatocytes and Hepatic Stellate Cells Carry Different Levels of <scp>DNA<\/scp> Damage due to Their Sensitivity to Oxidative Stress in Chronic Hepatitis B"],"prefix":"10.1111","volume":"32","author":[{"given":"Yansong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, The Fifth Affiliated Hospital Sun Yat\u2010Sen University  Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danjing","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The Fifth Affiliated Hospital Sun Yat\u2010Sen University  Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongqiong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The Fifth Affiliated Hospital Sun Yat\u2010Sen University  Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minyi","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The Fifth Affiliated Hospital Sun Yat\u2010Sen University  Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1971-6298","authenticated-orcid":false,"given":"Xiaomou","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The Fifth Affiliated Hospital Sun Yat\u2010Sen University  Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,24]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874357901812010026"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9071665"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-020-10125-y"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2024.155391"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.01.002"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9081870"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-998-3_40"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/597095"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3207"},{"key":"e_1_2_12_11_1","first-page":"305","article-title":"Gamma\u2010H2AX \u2013 A Novel Biomarker for DNA Double\u2010Strand Breaks","volume":"22","author":"Kuo L. J.","year":"2008","journal-title":"In Vivo"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1269\/jrr.45.473"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2019.102673"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.14802"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01091-7"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2019.109445"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2022.153397"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10071265"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2015.08.012"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-8278(91)90084-O"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-12-154"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.13884"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2004.01.008"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2013.1720"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.12-226639"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-018-0607-3"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2015.05.017"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02048"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13174258"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.22024"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795-009-0457-8"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801934200"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19040970"},{"key":"e_1_2_12_34_1","first-page":"771","article-title":"Cell Death Response to DNA Damage","volume":"92","author":"Wang J. Y. J.","year":"2019","journal-title":"Yale Journal of Biology and Medicine"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2020.188493"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI66369"}],"container-title":["Journal of Viral Hepatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jvh.70017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T11:42:21Z","timestamp":1741261341000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jvh.70017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,24]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1111\/jvh.70017"],"URL":"https:\/\/doi.org\/10.1111\/jvh.70017","archive":["Portico"],"relation":{},"ISSN":["1352-0504","1365-2893"],"issn-type":[{"value":"1352-0504","type":"print"},{"value":"1365-2893","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,24]]},"assertion":[{"value":"2024-12-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70017"}}