{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T05:03:15Z","timestamp":1785301395997,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hepatitis Delta Virus (HDV) infection in HBV\u2010infected individuals is associated with severe clinical outcomes, and screening is recommended in HBsAg\u2010positive patients. We used a rapid lateral flow assay (LFA) to assess anti\u2010HDV antibodies in Brazil, where HDV is highly endemic in the North, while data from the Southeast, particularly S\u00e3o Paulo, remain limited. We conducted a retrospective cross\u2010sectional study to assess anti\u2010HDV antibodies among 46 HBsAg\u2010positive blood donors at Funda\u00e7\u00e3o Pr\u00f3\u2010Sangue\/Hemocentro de S\u00e3o Paulo, 381 patients with chronic hepatitis B from the Central Laboratory Division of HC\u2010FMUSP, and 189 individuals from the Hepatology Clinic of HC\u2010FMUSP (101 HIV\u2010infected and 88 chronically HBV\u2010infected). Anti\u2010HDV antibodies were detected using a validated LFA. Positive controls (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200924) included samples previously confirmed by ELISA and\/or PCR, predominantly infected with HDV genotypes 3 and 8. Among 443,400 blood donations, 48 (0.01%) were positive for at least two HBV markers; of these, 46 were tested for HDV, and all were anti\u2010HDV negative. All 381 patients undergoing HBV viral load monitoring were anti\u2010HDV negative, as were all 189 patients receiving hepatology care, including those with HIV coinfection or advanced liver disease. The LFA correctly identified all control samples positive for both anti\u2010HDV antibodies and HDV RNA. The absence of detectable anti\u2010HDV among diverse at\u2010risk populations in S\u00e3o Paulo is consistent with the low endemicity of HDV infection in southeastern Brazil. These findings, together with the assay's high specificity in HDV RNA\u2010positive controls, support its potential utility as a screening tool for HDV infection in low\u2010prevalence settings.\n                  <\/jats:p>","DOI":"10.1111\/jvh.70217","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:53:59Z","timestamp":1785286439000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Absence of Detectable Anti\u2010\n                    <scp>HDV<\/scp>\n                    Antibodies Among\n                    <scp>HBsAg<\/scp>\n                    \u2010Positive Blood Donors,\n                    <scp>HIV<\/scp>\n                    \u2010Infected Individuals and Patients With Chronic Hepatitis B in Sao Paulo, Brazil: Evaluation Using a Rapid Lateral Flow Assay"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0623-1999","authenticated-orcid":false,"given":"Anna S.","family":"Nishiya","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Pr\u00f3\u2010Sangue\/Hemocentro de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Department of Hematology Laboratory of Medical Investigation in Pathogenesis and Targeted Therapy in Onco\u2010Immuno\u2010Hematology (LIM\u201031), HCFMUSP  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3583-2275","authenticated-orcid":false,"given":"Cecilia S.","family":"Alencar","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Medicina Laboratorial (LIM\u201003), HCFMUSP  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5932-2360","authenticated-orcid":false,"given":"Michele Soares Gomes","family":"Gouvea","sequence":"additional","affiliation":[{"name":"Gastroenterology and Tropical Hepatology Laboratory (LIM\/07) Institute of Tropical Medicine\/University of S\u00e3o Paulo Medical School  S\u00e3o Paulo Brazil"},{"name":"Department of Gastroenterology, Hospital das Cl\u00ednicas, School of Medicine University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzane K.","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital das Cl\u00ednicas, School of Medicine University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanci Alves","family":"Salles","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Pr\u00f3\u2010Sangue\/Hemocentro de S\u00e3o Paulo  S\u00e3o Paulo 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Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6688-3614","authenticated-orcid":false,"given":"Raymond F.","family":"Schinazi","sequence":"additional","affiliation":[{"name":"Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics Emory University School of Medicine, and Children's Healthcare of Atlanta  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leda","family":"Bassit","sequence":"additional","affiliation":[{"name":"Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics Emory University School of Medicine, and Children's Healthcare of Atlanta  Atlanta Georgia 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