{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:49:26Z","timestamp":1770752966441,"version":"3.50.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Little is known about the specificity of Bartonella spp. immunofluorescent antibody (IFA) assays in dogs. Bacteremia in sick dogs most often has been associated with Bartonella henselae (Bh), Bartonella vinsonii subspecies berkhoffii (Bvb), and Bartonella koehlerae (Bk). Clarification of the diagnostic utility of IFA serology when testing against these organisms is needed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the specificity of Bartonella IFA assays utilizing 6 cell culture\u2013grown antigen preparations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Archived sera from SPF dogs (n = 29) and from dogs experimentally infected with Bvb (n = 10) and Bh (n = 3).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Antibodies (Abs) to Bvb genotypes I, II, and III, Bh serotype I, strains H-1 and SA2, and to Bk were determined by IFA testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Serum from na\u00efve SPF dogs shown to be negative for Bartonella bacteremia did not react with any of the 6 Bartonella antigens by IFA testing. Dogs experimentally infected with Bvb genotype I developed Abs against homologous antigens, with no cross-reactivity to heterologous Bvb genotypes, Bh H-1, SA2 strains, or to Bk. Dogs experimentally infected with Bh serotype I developed Abs against Bh H-1, but not to Bh SA2 strain with no cross-reactive Abs to Bvb genotypes I\u2013III or to Bk.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>Bartonella spp. Ab responses during acute experimental infections are species and type specific.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.12263","type":"journal-article","created":{"date-parts":[[2013,12,16]],"date-time":"2013-12-16T13:01:02Z","timestamp":1387198862000},"page":"38-41","source":"Crossref","is-referenced-by-count":32,"title":["Analysis of Seroreactivity against Cell Culture\u2013Derived\n                    <i>Bartonella<\/i>\n                    spp. Antigens in Dogs"],"prefix":"10.1093","volume":"28","author":[{"given":"B.C.","family":"Hegarty","sequence":"first","affiliation":[{"name":"Intracellular Pathogens Research Laboratory, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, NCSU , Raleigh, NC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.M.","family":"Bradley","sequence":"additional","affiliation":[{"name":"Intracellular Pathogens Research Laboratory, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, NCSU , Raleigh, NC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.R.","family":"Lappin","sequence":"additional","affiliation":[{"name":"Colorado State University Department of Clinical Sciences, , Fort Collins, CO"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Balakrishnan","sequence":"additional","affiliation":[{"name":"Intracellular Pathogens Research Laboratory, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, NCSU , Raleigh, 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