{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T21:44:20Z","timestamp":1788212660567,"version":"build-2803163510"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":3747,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Hill's Provet AG Switzerland"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The ACTH stimulation test is used to evaluate the adrenocortical reserve. Recently, the availability of the synthetic ACTH formulation was limited, causing major problems in clinical practice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The objective of this study was to evaluate poststimulation peak cortisol concentrations and the duration of the stimulatory effect of a depot ACTH preparation in dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Twenty-two healthy dogs, 10 dogs with suspected hypoadrenocorticism (HA) and 15 dogs with suspected hyperadrenocorticism (HC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Prospective study. An ACTH stimulation test using a synthetic depot tetracosactide, administered intramuscularly (5\u00a0\u03bcg\/kg or at least 0.1\u00a0mL) was performed. Blood samples for determination of cortisol were taken immediately before and 1, 2, 3, 4, 6, and 24\u00a0hours after stimulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Peak cortisol concentrations were reached after 2\u20134\u00a0hours in all dogs. Cortisol concentrations 1\u00a0hour after stimulation were &amp;gt;9\u00a0\u03bcg\/dL in all healthy dogs and &amp;gt;5\u00a0\u03bcg\/dL in all dogs in which HA was excluded. None of the dogs with HA showed a cortisol-increase above the detection-limit of the assay. After 6\u00a0hours, cortisol concentrations had decreased in the healthy and HC group and were back to baseline after 24\u00a0hours.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>The depot formulation can be used in place of the short-acting ACTH to evaluate the adrenocortical reserve. Blood for peak cortisol concentrations should be drawn 3\u00a0hours after stimulation in cases in which HC is suspected; in HA-suspected cases, blood sampling can take place after 1\u00a0hour. As the stimulatory effect is gone after 24\u00a0hours, interference with other hormonal tests is unlikely after that time.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.13641","type":"journal-article","created":{"date-parts":[[2015,10,28]],"date-time":"2015-10-28T00:05:11Z","timestamp":1445990711000},"page":"1541-1546","source":"Crossref","is-referenced-by-count":13,"title":["Cortisol Response in Healthy and Diseased Dogs after Stimulation with a Depot Formulation of Synthetic ACTH"],"prefix":"10.1093","volume":"29","author":[{"given":"N.S.","family":"Sieber-Ruckstuhl","sequence":"first","affiliation":[{"name":"Clinic for Small Animal Internal Medicine, Vetsuisse Faculty University of Zurich , Zurich,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.A.","family":"Burkhardt","sequence":"additional","affiliation":[{"name":"Clinic for Small Animal Internal Medicine, Vetsuisse Faculty University of Zurich , 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