{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:19:50Z","timestamp":1790662790356,"version":"4.1.0"},"reference-count":10,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":3686,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Canine adenovirus 2, parainfluenza, and Bordetella bronchiseptica cause respiratory disease in dogs, and each has a modified live intranasal vaccine available. Molecular diagnostic assays to amplify specific nucleic acids are available for each of these agents. If positive molecular diagnostic assay results are common after vaccination, the positive predictive value of the diagnostic assays for disease would be decreased.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the impact of administration of commercially available modified live topical adenovirus 2, B. bronchiseptica, and parainfluenza vaccine has on the results of a commercially available PCR panel.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Eight puppies from a research breeding facility negative for these pathogens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blinded prospective pilot study. Puppies were vaccinated with a single dose of modified live topical adenovirus 2, B. bronchiseptica, and parainfluenza and parenteral dose of adenovirus 2, canine distemper virus, and parvovirus. Nasal and pharyngeal swabs were collected on multiple days and submitted for PCR assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Nucleic acids of all 3 organisms contained in the topical vaccine were detected from both samples multiple times through 28 days after vaccination with higher numbers of positive samples detected between days 3 and 10 after vaccination.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>Vaccine status should be considered when interpreting respiratory agent PCR results if modified live vaccines have been used. Development of quantitative PCR and wild-type sequencing are necessary to improve positive predictive value of these assays by distinguishing vaccinate from natural infection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.13821","type":"journal-article","created":{"date-parts":[[2015,12,22]],"date-time":"2015-12-22T08:01:59Z","timestamp":1450771319000},"page":"164-166","source":"Crossref","is-referenced-by-count":18,"title":["Adenovirus 2,\n                    <i>Bordetella bronchiseptica<\/i>\n                    , and Parainfluenza Molecular Diagnostic Assay Results in Puppies After vaccination with Modified Live Vaccines"],"prefix":"10.1093","volume":"30","author":[{"given":"R.","family":"Ruch-Gallie","sequence":"first","affiliation":[{"name":"Colorado State University Center for Companion Animal Studies and the Department of Clinical Sciences, , Ft. Collins, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Moroff","sequence":"additional","affiliation":[{"name":"Antech Diagnostics , Lake Success, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.R.","family":"Lappin","sequence":"additional","affiliation":[{"name":"Colorado State University Center for Companion Animal Studies and the Department of Clinical Sciences, , Ft. Collins, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,1,1]]},"reference":[{"key":"2026020304495995700_jvim13821-bib-0001","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1177\/0300985813511130","article-title":"New and emerging pathogens in canine infectious respiratory disease","volume":"51","author":"Priestnall","year":"2014","journal-title":"Vet Pathol"},{"key":"2026020304495995700_jvim13821-bib-0002","first-page":"902","article-title":"Bordetella infections in dogs and cats: Treatment and prevention","volume":"25","author":"Datz","year":"2003","journal-title":"Compen Contin Educ Vet"},{"key":"2026020304495995700_jvim13821-bib-0003","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1177\/0300985813511129","article-title":"Common and emerging infectious diseases in the animal shelter","volume":"51","author":"Pesavento","year":"2014","journal-title":"Vet Pathol"},{"key":"2026020304495995700_jvim13821-bib-0004","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.1007\/s007050050250","article-title":"Effect of a live attenuated intranasal vaccine on latency and shedding of feline herpesvirus 1 in domestic cats","volume":"142","author":"Weigler","year":"1997","journal-title":"Arch Virol"},{"key":"2026020304495995700_jvim13821-bib-0005","doi-asserted-by":"crossref","first-page":"4322","DOI":"10.1016\/j.vaccine.2011.04.022","article-title":"Shedding of Ann Arbor strain live attenuated influenza vaccine virus in children 6-59 months of age","volume":"29","author":"Mallory","year":"2011","journal-title":"Vaccine"},{"key":"2026020304495995700_jvim13821-bib-0006","doi-asserted-by":"crossref","first-page":"479","DOI":"10.2478\/bvip-2013-0083","article-title":"Shedding course of bovine respiratory syncytial virus and bovine parainfluenza 3 virus in calves vaccinated intranasally","volume":"57","author":"Socha","year":"2013","journal-title":"Bull Vet Inst Pulawy"},{"key":"2026020304495995700_jvim13821-bib-0007","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.2460\/ajvr.1992.53.11.2164","article-title":"Effect of various vaccination procedures on shedding, latency, and reactivation of attenuated and virulent pseudorabies virus in swine","volume":"53","author":"Mengeling","year":"1992","journal-title":"Am J Vet Res"},{"key":"2026020304495995700_jvim13821-bib-0008","doi-asserted-by":"crossref","first-page":"3850","DOI":"10.1016\/j.vaccine.2014.04.050","article-title":"Long-term viremia and fecal shedding in pups after modified-live canine parvovirus vaccination","volume":"32","author":"Decaro","year":"2014","journal-title":"Vaccine"},{"key":"2026020304495995700_jvim13821-bib-0009","first-page":"747","article-title":"Simultaneous analysis of the nasal shedding kinetics of field and vaccine strains of Bordetella bronchiseptica","volume":"165","author":"Iemura","year":"2009","journal-title":"Vet Rec"},{"key":"2026020304495995700_jvim13821-bib-0010","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1177\/1040638713517232","article-title":"Real-time reverse transcription polymerase chain reaction method for detection of Canine distemper virus modified live vaccine shedding for differentiation from infection with wild-type strains","volume":"26","author":"Wilkes","year":"2014","journal-title":"J Vet Diagn Invest"}],"container-title":["Journal of Veterinary Internal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjvim.13821","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jvim.13821","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jvim.13821","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jvim\/article-pdf\/30\/1\/164\/66690030\/jvim13821.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jvim\/article-pdf\/30\/1\/164\/66690030\/jvim13821.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T09:50:21Z","timestamp":1770112221000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jvim\/article\/30\/1\/164\/8452824"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,1]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1,1]]}},"URL":"https:\/\/doi.org\/10.1111\/jvim.13821","relation":{},"ISSN":["1939-1676"],"issn-type":[{"value":"1939-1676","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2016,1]]},"published":{"date-parts":[[2016,1,1]]}}}