{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:03:08Z","timestamp":1790535788586,"version":"4.1.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":3077,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Dianov Laboratories"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["OD 010939"],"award-info":[{"award-number":["OD 010939"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>When dogs are transfused, blood compatibility testing varies widely but may include dog erythrocyte antigen (DEA) 1 typing and rarely cross-matching.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Prospective study to examine naturally occurring alloantibodies against red blood cells (RBCs) and alloimmunization by transfusion using 2 antiglobulin-enhanced cross-match tests.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Eighty client-owned anemic, 72 donor, and 7 control dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>All dogs were typed for DEA 1 and some also for DEA 4 and DEA 7. Major cross-match tests with canine antiglobulin-enhanced immunochromatographic strip and gel columns were performed 26\u2013129 days post-transfusion (median, 39 days); some dogs had an additional early evaluation 11\u201322 days post-transfusion (median, 16 days). Plasma from alloimmunized recipients was cross-matched against RBCs from 34 donor and control dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The 2 cross-match methods gave entirely concordant results. All 126 pretransfusion cross-match results for the 80 anemic recipients were compatible, but 54 dogs died or were lost to follow up. Among the 26 recipients with follow-up, 1 dog accidently received DEA 1-mismatched blood and became cross-match-incompatible post-transfusion. Eleven of the 25 DEA 1-matched recipients (44%) became incompatible against other RBC antigens. No naturally occurring anti-DEA 7 alloantibodies were detected in DEA 7\u2212 dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and clinical importance<\/jats:title>\n                    <jats:p>The antiglobulin-enhanced immunochromatographic strip cross-match and laboratory gel column techniques identified no naturally occurring alloantibodies against RBC antigens, but a high degree of post-transfusion alloimmunization in dogs. Cross-matching is warranted in any dog that has been previously transfused independent of initial DEA 1 typing and cross-matching results before the first transfusion event.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.14801","type":"journal-article","created":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T02:09:03Z","timestamp":1502676543000},"page":"1420-1429","source":"Crossref","is-referenced-by-count":34,"title":["Pre- and Post-Transfusion Alloimmunization in Dogs Characterized by 2 Antiglobulin-Enhanced Cross-match Tests"],"prefix":"10.1093","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0531-7827","authenticated-orcid":false,"given":"I.","family":"Goy-Thollot","sequence":"first","affiliation":[{"name":"SIAMU, VetAgro Sup, University of Lyon, APCSe , Marcy l'Etoile,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Giger","sequence":"additional","affiliation":[{"name":"Section of Medical Genetics (PennGen), University of Pennsylvania , Philadelphia, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Boisvineau","sequence":"additional","affiliation":[{"name":"SIAMU, VetAgro Sup, University of Lyon, APCSe , Marcy l'Etoile,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Perrin","sequence":"additional","affiliation":[{"name":"SIAMU, VetAgro Sup, University of Lyon, APCSe , Marcy l'Etoile,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Guidetti","sequence":"additional","affiliation":[{"name":"Dianov Laboratories , Limonest,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Chaprier","sequence":"additional","affiliation":[{"name":"Dianov Laboratories , Limonest,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Barth\u00e9lemy","sequence":"additional","affiliation":[{"name":"SIAMU, VetAgro Sup, University of Lyon, APCSe , Marcy l'Etoile,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Pouzot-Nevoret","sequence":"additional","affiliation":[{"name":"SIAMU, VetAgro Sup, University of Lyon, APCSe , Marcy l'Etoile,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Canard","sequence":"additional","affiliation":[{"name":"Dianov Laboratories , Limonest,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,9,1]]},"reference":[{"key":"2026020303211580300_jvim14801-bib-0001","first-page":"e143","article-title":"Kirk's Current Veterinary Therapy","author":"Giger","year":"2014","edition":"15"},{"key":"2026020303211580300_jvim14801-bib-0002","doi-asserted-by":"crossref","first-page":"431","DOI":"10.2460\/javma.244.4.431","article-title":"Risk factors for transfusion-associated complications and nonsurvival in dogs receiving packed red blood cell transfusions: 211 cases (2008-2011)","volume":"244","author":"Holowaychuk","year":"2014","journal-title":"J Am Vet Med Assoc"},{"key":"2026020303211580300_jvim14801-bib-0003","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1111\/j.1939-165X.2010.00249.x","article-title":"Dog erythrocyte antigens 1.1, 1.2, 3, 4, 7, and Dal blood typing and cross-matching by gel column technique","volume":"39","author":"Kessler","year":"2010","journal-title":"Vet Clin Pathol"},{"key":"2026020303211580300_jvim14801-bib-0004","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1111\/jvim.12321","article-title":"DEA 1 expression on dog erythrocytes analyzed by immunochromatographic and flow cytometric techniques","volume":"28","author":"Acierno","year":"2014","journal-title":"J Vet Intern Med"},{"key":"2026020303211580300_jvim14801-bib-0005","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.2460\/javma.1995.206.09.1358","article-title":"An acute hemolytic transfusion reaction caused by dog erythrocyte antigen 1.1 incompatibility in a previously sensitized dog","volume":"206","author":"Giger","year":"1995","journal-title":"J Am Vet Med Assoc"},{"key":"2026020303211580300_jvim14801-bib-0006","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.2460\/ajvr.2005.66.1386","article-title":"Comparison of various canine blood-typing methods","volume":"66","author":"Giger","year":"2005","journal-title":"Am J Vet Res"},{"key":"2026020303211580300_jvim14801-bib-0007","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1111\/vcp.12284","article-title":"Dog erythrocyte antigen 1: Mode of inheritance and initial characterization","volume":"44","author":"Polak","year":"2015","journal-title":"Vet Clin Pathol"},{"key":"2026020303211580300_jvim14801-bib-0008","doi-asserted-by":"crossref","first-page":"213","DOI":"10.2460\/ajvr.73.2.213","article-title":"Comparison of gel column, card, and cartridge techniques for dog erythrocyte antigen 1.1 blood typing","volume":"73","author":"Seth","year":"2012","journal-title":"Am J Vet Res"},{"key":"2026020303211580300_jvim14801-bib-0009","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1111\/j.1939-1676.2007.tb02961.x","article-title":"Canine Dal blood type: A red cell antigen lacking in some Dalmatians","volume":"21","author":"Blais","year":"2007","journal-title":"J Vet Intern Med"},{"key":"2026020303211580300_jvim14801-bib-0010","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1111\/jvim.14572","article-title":"Survey of two new (Kai 1 and Kai 2) and other blood groups in dogs of North America","volume":"30","author":"Euler","year":"2016","journal-title":"J Vet Intern Med"},{"key":"2026020303211580300_jvim14801-bib-0011","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.tmrv.2003.12.003","article-title":"Importance of blood groups and blood group antibodies in companion animals","volume":"18","author":"Hohenhaus","year":"2004","journal-title":"Transfus Med Rev"},{"key":"2026020303211580300_jvim14801-bib-0012","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1111\/jvim.14693","article-title":"Prevalence and mode of inheritance of the Dal blood group in dogs in North America","volume":"31","author":"Goulet","year":"2017","journal-title":"J Vet Intern Med"},{"key":"2026020303211580300_jvim14801-bib-0013","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1111\/j.1939-1676.2009.0286.x","article-title":"Lack of evidence of pregnancy-induced alloantibodies in dogs","volume":"23","author":"Blais","year":"2009","journal-title":"J Vet Intern Med"},{"key":"2026020303211580300_jvim14801-bib-0014","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1177\/1040638716668626","article-title":"Activity, specificity, and titer of naturally occurring canine anti-DEA 7 antibodies","volume":"28","author":"Spada","year":"2016","journal-title":"J Vet Diagn Invest"},{"key":"2026020303211580300_jvim14801-bib-0015","doi-asserted-by":"crossref","first-page":"877","DOI":"10.2460\/ajvr.77.8.877","article-title":"Prevalence of naturally occurring antibodies against dog erythrocyte antigen 7 in a population of dog erythrocyte antigen 7-negative dogs from Spain and Italy","volume":"77","author":"Spada","year":"2016","journal-title":"Am J Vet Res"},{"key":"2026020303211580300_jvim14801-bib-0016","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/j.1939-1676.1995.tb01080.x","article-title":"Hemolytic transfusion reactions in a dog with an alloantibody to a common antigen","volume":"9","author":"Callan","year":"1995","journal-title":"J Vet Intern Med"},{"key":"2026020303211580300_jvim14801-bib-0017","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1111\/j.1939-1676.2003.tb02536.x","article-title":"A hemolytic transfusion reaction due to DEA 4 alloantibodies in a dog","volume":"17","author":"Melzer","year":"2003","journal-title":"J Vet Intern Med"},{"key":"2026020303211580300_jvim14801-bib-0018","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1111\/vec.12433","article-title":"Comparison of a commercial blood cross-matching kit to the standard laboratory method for establishing blood transfusion compatibility in dogs","volume":"26","author":"Guzman","year":"2016","journal-title":"J Vet Emerg Crit Care (San Antonio)"},{"key":"2026020303211580300_jvim14801-bib-0019","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/vcp.12374","article-title":"Preliminary evaluation of a gel tube agglutination major cross-match method in dogs","volume":"45","author":"Villarnovo","year":"2016","journal-title":"Vet Clin Pathol"},{"key":"2026020303211580300_jvim14801-bib-0020","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/jvim.12292","article-title":"Comparison of 4 direct Coombs' test methods with polyclonal antiglobulins in anemic and nonanemic dogs for in-clinic or laboratory use","volume":"28","author":"Caviezel","year":"2014","journal-title":"J Vet Intern Med"},{"key":"2026020303211580300_jvim14801-bib-0021","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1046\/j.1537-2995.1990.30290162894.x","article-title":"The gel test: A new way to detect red cell antigen-antibody reactions","volume":"30","author":"Lapierre","year":"1990","journal-title":"Transfusion"},{"key":"2026020303211580300_jvim14801-bib-0022","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.transci.2014.08.025","article-title":"Antibody screening in multitransfused patients: A prerequisite before each transfusion","volume":"51","author":"Lamba","year":"2014","journal-title":"Transfus Apher Sci"},{"key":"2026020303211580300_jvim14801-bib-0023","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1002\/jcla.20033","article-title":"Comparison of conventional tube test technique and gel microcolumn assay for direct antiglobulin test: A large study","volume":"18","author":"Novaretti","year":"2004","journal-title":"J Clin Lab Anal"},{"key":"2026020303211580300_jvim14801-bib-0024","first-page":"280","article-title":"BSAVA Manual of Canine and Feline Haematology and Transfusion Medicine","author":"Hale","year":"2012","edition":"2"},{"key":"2026020303211580300_jvim14801-bib-0025","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/S0195-5616(95)50157-3","article-title":"Canine blood groups and their importance in veterinary transfusion medicine","volume":"25","author":"Hale","year":"1995","journal-title":"Vet Clin North Am Small Anim Pract"},{"key":"2026020303211580300_jvim14801-bib-0026","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1111\/j.1748-5827.2011.01103.x","article-title":"Production of an anticanine globulin (polyspecific) reagent for laboratory investigation","volume":"52","author":"Sriwichai","year":"2011","journal-title":"J Small Anim Pract"},{"key":"2026020303211580300_jvim14801-bib-0027","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1111\/tme.12380","article-title":"SHOT conference report 2016: Serious hazards of transfusion \u2013 human factors continue to cause most transfusion-related incidents","volume":"26","author":"Bolton-Maggs","year":"2016","journal-title":"Transfus Med"},{"key":"2026020303211580300_jvim14801-bib-0028","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1097\/01.moh.0000245694.70135.c3","article-title":"How I manage donors and patients with a weak D phenotype","volume":"13","author":"Flegel","year":"2006","journal-title":"Curr Opin Hematol"},{"key":"2026020303211580300_jvim14801-bib-0029","first-page":"22","article-title":"Transfusion of RhD-incompatible blood components in RhD-negative blood marrow transplant recipients","volume":"6","author":"Asfour","year":"2004","journal-title":"MedGenMed"},{"key":"2026020303211580300_jvim14801-bib-0030","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1111\/j.1423-0410.2011.01517.x","article-title":"Red-blood-cell alloimmunization and number of red-blood-cell transfusions","volume":"102","author":"Zalpuri","year":"2012","journal-title":"Vox Sang"},{"key":"2026020303211580300_jvim14801-bib-0031","doi-asserted-by":"crossref","first-page":"e284","DOI":"10.1016\/S2352-3026(16)30019-9","article-title":"Red-blood-cell alloimmunisation in relation to antigens' exposure and their immunogenicity: A cohort study","volume":"3","author":"Evers","year":"2016","journal-title":"Lancet Haematol"},{"key":"2026020303211580300_jvim14801-bib-0032","first-page":"946","article-title":"Post-transfusion survival of fresh and stored dog erythrocytes measured by differential agglutination","volume":"41","author":"Swisher","year":"1953","journal-title":"J Lab Clin Med"}],"container-title":["Journal of Veterinary Internal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjvim.14801","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jvim\/article-pdf\/31\/5\/1420\/66672277\/jvim14801.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jvim\/article-pdf\/31\/5\/1420\/66672277\/jvim14801.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T08:21:25Z","timestamp":1770106885000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jvim\/article\/31\/5\/1420\/8449344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,1]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,9,1]]}},"URL":"https:\/\/doi.org\/10.1111\/jvim.14801","relation":{},"ISSN":["1939-1676"],"issn-type":[{"value":"1939-1676","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2017,9]]},"published":{"date-parts":[[2017,9,1]]}}}