{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T02:14:29Z","timestamp":1776132869876,"version":"3.50.1"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":3016,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006306","name":"Sigrid Jus\u00e9lius Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006306","id-type":"DOI","asserted-by":"publisher"}]},{"name":"State Subsidy for Health Research"},{"DOI":"10.13039\/501100004325","name":"Signe and Ane Gyllenberg Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004325","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Helvi Knuuttila Foundation"},{"DOI":"10.13039\/501100006515","name":"Finnish Veterinary Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006515","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004756","name":"Emil Aaltonen Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004756","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Immune system involvement is suggested as an underlying cause for Doberman hepatitis (DH) based on female predisposition, lymphocyte infiltration, abnormal hepatocyte expression of major histocompatibility complex class II antigens, and homozygosity for dog leukocyte antigen DRB1*00601.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To measure serum antinuclear antibodies (ANA) and serum antihistone antibodies (AHA) in Dobermans with hepatitis. To determine whether increased serum ANA or serum AHA could be used to support the diagnosis of Doberman hepatitis (DH).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Privately owned 25 subclinically and 13 clinically affected DH Dobermans and 17 healthy control Dobermans.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Case\u2013control study. Indirect immunofluorescence (IIF) microscopy and line blot tests were employed for the ANA pilot studies and an enzyme-linked immunosorbent assay (ELISA) assay for detection of IgG AHA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Indirect immunofluorescence revealed ANA-positive cases, and line blot showed AHA reactivity. In ELISA, importantly increased concentrations of AHA were found in 92% (23\/25) of dogs in the subclinical stage and 84.6% (11 of 13) of dogs in the clinical stage of DH compared with no control dogs (0\/17) (P &amp;lt; 0.0005). The mean AHA absorbance values of the blood samples obtained from the 25 subclinical DH dogs (1.36 \u00b1 0.60, mean \u00b1 SD) and the 13 clinically affected dogs (1.46 \u00b1 0.49) were significantly higher than in 17 control dogs (0.51 \u00b1 0.18; P &amp;lt; 0.0001).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>As the presence of AHA indicates autoimmune activity, our results favor an autoimmune background as one cause for DH. Antihistone antibody could represent a novel means for screening Dobermans with increased serum alanine transaminase concentrations and suspicion of DH.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.14838","type":"journal-article","created":{"date-parts":[[2017,9,30]],"date-time":"2017-09-30T09:36:36Z","timestamp":1506764196000},"page":"1717-1723","source":"Crossref","is-referenced-by-count":12,"title":["Antihistone Autoantibodies in Dobermans With Hepatitis"],"prefix":"10.1093","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9712-9753","authenticated-orcid":false,"given":"H.","family":"Dyggve","sequence":"first","affiliation":[{"name":"University of Helsinki Department of Equine and Small Animal Medicine, , Helsinki,","place":["Finland"]},{"name":"University of Helsinki and HUSLAB Department of Bacteriology and Immunology and Immunobiology Research Program, , Helsinki,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Meri","sequence":"additional","affiliation":[{"name":"University of Helsinki and HUSLAB Department of Bacteriology and Immunology and Immunobiology Research Program, , Helsinki,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Spillmann","sequence":"additional","affiliation":[{"name":"University of Helsinki Department of Equine and Small Animal Medicine, , Helsinki,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Jarva","sequence":"additional","affiliation":[{"name":"University of Helsinki and HUSLAB Department of Bacteriology and Immunology and Immunobiology Research Program, , Helsinki,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Speeti","sequence":"additional","affiliation":[{"name":"University of Helsinki Department of Equine and Small Animal Medicine, , Helsinki,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,11,1]]},"reference":[{"key":"2026020303231310000_jvim14838-bib-0001","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1111\/j.1748-5827.1996.tb01741.x","article-title":"Subclinical versus clinical hepatitis in the Doberman: Evaluation of changes in blood parameters","volume":"37","author":"Speeti","year":"1996","journal-title":"J Small Anim Pract"},{"key":"2026020303231310000_jvim14838-bib-0002","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1177\/030098589803500505","article-title":"Lesions of subclinical hepatitis","volume":"35","author":"Speeti","year":"1998","journal-title":"Vet Pathol"},{"key":"2026020303231310000_jvim14838-bib-0003","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1080\/01652176.2004.9695173","article-title":"Chronic hepatitis in Doberman pinschers. 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