{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T20:59:15Z","timestamp":1770152355341,"version":"3.49.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":2955,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005232","name":"Maddie's Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005232","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Lois Kugler Small Animal Research Trust"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Dogs seized by law enforcement agencies during dogfighting investigations are at increased risk of Babesia gibsoni infection. A rapid and cost-effective diagnostic test would increase the feasibility of mass screening of dogs for infection and monitoring treatment efficacy in B.\u00a0gibsoni-infected dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the performance of a point-of-need insulated isothermal PCR (iiPCR) test for diagnosis of B.\u00a0gibsoni in dogs rescued in dogfighting investigations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Two hundred and thirty-three dogs seized in dogfighting investigations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cross-sectional study. Whole blood samples were tested for B.\u00a0gibsoni and Babesia spp. by iiPCR. Results were compared to a reference standard comprised of concordant results from real-time PCR in a commercial diagnostic laboratory and antibody titers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The iiPCR system was quick to learn, portable, and had a short processing time of &amp;lt;2\u00a0hours. Sensitivity and specificity of the iiPCR assay for B.\u00a0gibsoni were 90% (95% confidence interval [CI] 81\u201395%) and 99% (CI, 95\u2013100%), respectively. Sensitivity and specificity of the iiPCR assay for Babesia spp. were 87% (CI, 78\u201393%) and 98% (CI, 0.94\u201399%), respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>The iiPCR system produced few false-positive results, indicating that positive results are likely to represent true infections when used in high-risk animals. The iiPCR system can fail to identify 10\u201315% of truly infected dogs. However, the portability, speed, and economy of the iiPCR system compared to testing through a reference laboratory can allow rescue groups to screen and identify infection in more dogs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.15033","type":"journal-article","created":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T14:03:44Z","timestamp":1517234624000},"page":"232-235","source":"Crossref","is-referenced-by-count":5,"title":["Rapid Diagnosis of\n                    <i>Babesia gibsoni<\/i>\n                    by Point-of-Need Testing by Insulated Isothermal PCR in Dogs at High Risk of Infection"],"prefix":"10.1093","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9260-2564","authenticated-orcid":false,"given":"K.L.","family":"Cooke","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, University of Florida Department of Small Animal Clinical Sciences, , Gainesville, 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