{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:59:47Z","timestamp":1781107187915,"version":"3.54.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":2531,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2018,12,17]],"date-time":"2018-12-17T00:00:00Z","timestamp":1545004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Blood typing for the A and B antigens is essential and crossmatching testing is generally recommended before transfusing blood to cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate 2 crossmatch (XM) tests.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Forty-nine healthy domestic shorthair cats that had not received a blood transfusion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Prospective study. Blood samples were typed for AB using immunochromatographic and flow cytometric techniques. A gel column (GC) and a feline antiglobulin-enhanced gel column (AGC) XM tests were used for crossmatching.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The population included 34 type A, 13 B, and 2 AB cats, with concordant results (r\u2009=\u20091, P &amp;lt;\u2009.005) by flow cytometry and immunochromatographic strip kit. The plasma from type A cats had either no or weak anti-B alloantibodies. The plasma of 12 of 13 type B cats contained strong anti-A alloantibodies. For crossmatching, plasma to RBC pairings were prepared using the GC (n\u2009=\u2009446) and AGC (n\u2009=\u2009630) tests. Both methods showed compatibilities in 329 and incompatibilities in 102 pairings including all A-B mismatches. Additionally 15 pairings showed agglutination by the AGC but not GC method. Fourteen incompatibilities outside the expected A-B mismatches were only revealed by AGC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>AB typing using immunochromatographic strip is as accurate as laboratory flow cytometry. The 2 XM methods had good agreement with additional incompatibilities being recognized by the AGC XM beyond A-B incompatibilities. In clinic, feline AB typing and sensitive XM test kits are available and recommended before each transfusion, although the clinical implications of incompatible XM test results and clinical benefits of such crossmatching have not been documented.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.15381","type":"journal-article","created":{"date-parts":[[2018,12,17]],"date-time":"2018-12-17T12:43:51Z","timestamp":1545050631000},"page":"588-595","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Detection of naturally occurring alloantibody by an in-clinic antiglobulin-enhanced and standard crossmatch gel column test in non-transfused domestic shorthair cats"],"prefix":"10.1093","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0531-7827","authenticated-orcid":false,"given":"Isabelle","family":"Goy-Thollot","sequence":"first","affiliation":[{"name":"SIAMU (ICU), Universit\u00e9 de Lyon, VetAgro Sup, APCSe , Marcy l'Etoile,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Nectoux","sequence":"additional","affiliation":[{"name":"SIAMU (ICU), Universit\u00e9 de Lyon, VetAgro Sup, APCSe , Marcy l'Etoile,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryline","family":"Guidetti","sequence":"additional","affiliation":[{"name":"Dianov Laboratories , Limonest,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Chaprier","sequence":"additional","affiliation":[{"name":"Dianov Laboratories , Limonest,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Bourgeois","sequence":"additional","affiliation":[{"name":"Dianov Laboratories , Limonest,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Boisvineau","sequence":"additional","affiliation":[{"name":"SIAMU (ICU), Universit\u00e9 de Lyon, VetAgro Sup, APCSe , Marcy l'Etoile,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9570-100X","authenticated-orcid":false,"given":"Anthony","family":"Barth\u00e9lemy","sequence":"additional","affiliation":[{"name":"SIAMU (ICU), Universit\u00e9 de Lyon, VetAgro Sup, APCSe , Marcy l'Etoile,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Pouzot-Nevoret","sequence":"additional","affiliation":[{"name":"SIAMU (ICU), Universit\u00e9 de Lyon, VetAgro Sup, APCSe , Marcy l'Etoile,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4770-4107","authenticated-orcid":false,"given":"Urs","family":"Giger","sequence":"additional","affiliation":[{"name":"Section of Medical Genetics (PennGen), University of Pennsylvania , Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,3,1]]},"reference":[{"key":"2026020922222398300_jvim15381-bib-0001","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.2460\/ajvr.1996.57.10.1438","article-title":"Blood type AB in the feline AB blood group system","volume":"57","author":"Griot-Wenk","year":"1996","journal-title":"Am J Vet Res"},{"key":"2026020922222398300_jvim15381-bib-0002","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/j.1751-0813.2005.tb12190.x","article-title":"The prevalence of feline A\/B blood types in the Sydney region","volume":"83","author":"Malik","year":"2005","journal-title":"Aust Vet J"},{"key":"2026020922222398300_jvim15381-bib-0003","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0204287","article-title":"Molecular characterization of blood type A, B, and C (AB) in domestic cats and a CMAH genotyping scheme","author":"Kehl","year":"2018","journal-title":"PLoS ONE"},{"key":"2026020922222398300_jvim15381-bib-0004","doi-asserted-by":"crossref","first-page":"e0154973","DOI":"10.1371\/journal.pone.0154973","article-title":"A novel variant in CMHA is associated with blood type AB in ragdoll cats","volume":"11","author":"Gandolfi","year":"2016","journal-title":"PLoS One"},{"key":"2026020922222398300_jvim15381-bib-0005","first-page":"e143","article-title":"Current Veterinary Therapy","author":"Giger","year":"2014"},{"key":"2026020922222398300_jvim15381-bib-0006","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1111\/j.1939-1676.2007.tb02962.x","article-title":"A newly recognized blood group in domestic shorthair cats: the Mik red cell antigen","volume":"21","author":"Weinstein","year":"2007","journal-title":"J Vet Intern Med"},{"key":"2026020922222398300_jvim15381-bib-0007","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/jsap.12180","article-title":"Feline blood genotyping versus phenotyping, and detection of non-AB blood type incompatibilities in UKcats","volume":"55","author":"Tasker","year":"2014","journal-title":"J Small Anim Pract"},{"key":"2026020922222398300_jvim15381-bib-0008","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/j.1939-1676.1990.tb03129.x","article-title":"Acute hemolytic transfusion reaction in an abyssinian cat with blood type B","volume":"4","author":"Giger","year":"1990","journal-title":"J Vet Intern Med"},{"key":"2026020922222398300_jvim15381-bib-0009","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0165-2427(93)90088-L","article-title":"Alloantibodies against A and B blood types in cats","volume":"38","author":"B\u00fccheler","year":"1993","journal-title":"Vet Immunol Immunopathol"},{"key":"2026020922222398300_jvim15381-bib-0010","first-page":"322","article-title":"Manual of Veterinary Transfusion Medicine and Blood Banking","author":"Abrams-Ogg","year":"2016"},{"key":"2026020922222398300_jvim15381-bib-0011","doi-asserted-by":"crossref","first-page":"203","DOI":"10.2460\/ajvr.72.2.203","article-title":"Comparison of five blood-typing methods for the feline AB blood group system","volume":"72","author":"Seth","year":"2011","journal-title":"Am J Vet Res"},{"key":"2026020922222398300_jvim15381-bib-0012","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/vec.12360","article-title":"Evaluation of an immunochromatographic test for feline AB system blood typing","volume":"26","author":"Spada","year":"2016","journal-title":"J Vet Emerg Crit Care"},{"key":"2026020922222398300_jvim15381-bib-0013","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1177\/1098612X14522052","article-title":"Evaluation of a novel feline AB blood typing device","volume":"16","author":"Hourani","year":"2014","journal-title":"J Feline Med Surg"},{"key":"2026020922222398300_jvim15381-bib-0014","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1111\/vcp.12366","article-title":"Xenotransfusion of anemic cats with blood compatibility issues: pre- and posttransfusion laboratory diagnostic and crossmatching studies","volume":"45","author":"Euler","year":"2016","journal-title":"Vet Clin Pathol"},{"key":"2026020922222398300_jvim15381-bib-0015","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1111\/vec.12565","article-title":"Ultrasound-guided intracardiac xenotransfusion of canine packed red blood cells and epinephrine to the left ventricule of a severely anemic cat during cardiopulmonary resuscitation","volume":"27","author":"Ron","year":"2017","journal-title":"J Vet Emerg Crit Care"},{"key":"2026020922222398300_jvim15381-bib-0016","doi-asserted-by":"crossref","DOI":"10.1177\/1098612X17727232","article-title":"Naturally occuring antibodies in cats against dog erythrocyte antigens and vice versa","author":"Priolo","year":"2018","journal-title":"J Feline Med Surg"},{"key":"2026020922222398300_jvim15381-bib-0017","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1177\/1098612X16688574","article-title":"Alloimmunisation in transfused patients: serial cross-matching in a population of hospitalised cats","volume":"19","author":"Hourani","year":"2017","journal-title":"J Feline Med Surg"},{"key":"2026020922222398300_jvim15381-bib-0018","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1111\/vec.12204","article-title":"Influence of cross-match on posttransfusion packed cell volume in feline packed red blood cell transfusion","volume":"24","author":"Weltman","year":"2014","journal-title":"J Vet Emerg Crit Care"},{"key":"2026020922222398300_jvim15381-bib-0019","article-title":"Incompatible crossmatch results related to factors other than A, B, and AB blood types in cats","volume":"S10","author":"Specht","year":"2017","journal-title":"J Vet Emerg Crit Care"},{"key":"2026020922222398300_jvim15381-bib-0020","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1111\/jvim.12321","article-title":"DEA 1 expression on dog erythrocytes analyzed immunochromatographic and flow cytometrc techniques","volume":"28","author":"Acierno","year":"2014","journal-title":"J Vet Intern Med"},{"key":"2026020922222398300_jvim15381-bib-0021","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1111\/jvim.14801","article-title":"Pre- and post-transfusion alloimmunisation in dogs characterized by two antiglobulin-enhanced crossmatch tests","volume":"31","author":"Goy-Thollot","year":"2017","journal-title":"J Vet Intern Med"},{"key":"2026020922222398300_jvim15381-bib-0022","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1046\/j.1537-2995.1990.30290162894.x","article-title":"The gel test: a new way to detect red cell antigen-antibody reactions","volume":"30","author":"Lapierre","year":"1990","journal-title":"Transfusion"},{"key":"2026020922222398300_jvim15381-bib-0023","doi-asserted-by":"crossref","first-page":"138","DOI":"10.21307\/immunohematology-2019-595","article-title":"Comparison of tube and gel techniques for antibody identification","volume":"16","author":"Novaretti","year":"2000","journal-title":"Immunohematology"},{"key":"2026020922222398300_jvim15381-bib-0024","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1002\/jcla.20033","article-title":"Comparison of conventional tube test technique and gel microcolumn assay for direct antiglobulin test: a large study","volume":"18","author":"Novaretti","year":"2004","journal-title":"J Clin Lab Anal"},{"key":"2026020922222398300_jvim15381-bib-0025","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/j.1476-4431.2009.00387.x","article-title":"Increasing patient safety in veterinary transfusion medicine: an overview of pretransfusion testing","volume":"19","author":"Tocci","year":"2009","journal-title":"J Vet Emerg Crit Care"},{"key":"2026020922222398300_jvim15381-bib-0026","first-page":"1","article-title":"Effect of cross-match on packed cell volume after transfusion of packed red blood cells in transfusion-na\u00efve anemic cats","volume":"00","author":"Sylvane","year":"2018","journal-title":"J Vet Intern Med"},{"key":"2026020922222398300_jvim15381-bib-0027","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.transci.2014.08.025","article-title":"Antibody screening in multitransfused patients: a prerequisite before each transfusion","volume":"51","author":"Lamba","year":"2014","journal-title":"Transfus Apher Sci"},{"key":"2026020922222398300_jvim15381-bib-0028","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/j.1939-1676.1995.tb01080.x","article-title":"Hemolytic transfusion reactions in a dog with an alloantibody to a common antigen","volume":"9","author":"Callan","year":"1995","journal-title":"J Vet Intern Med"},{"key":"2026020922222398300_jvim15381-bib-0029","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.cvsm.2013.03.007","article-title":"Transfusion medicine in small animals","volume":"43","author":"Davidow","year":"2013","journal-title":"Vet Clin N Am Small"},{"key":"2026020922222398300_jvim15381-bib-0030","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1046\/j.1537-2995.1976.16577039296.x","article-title":"Hemagglutination inhibition studies of water soluble blood group substance recovered from the erythrocytes of classical Bombay Oh subjects","volume":"16","author":"Vos","year":"1976","journal-title":"Transfusion"},{"key":"2026020922222398300_jvim15381-bib-0031","doi-asserted-by":"crossref","first-page":"208","DOI":"10.3343\/alm.2013.33.3.208","article-title":"A case of IgM deficiency with B cell deficiency detected by ABO discrepancy in a patient with acute osteomyelitis","volume":"33","author":"Jung","year":"2013","journal-title":"Ann Lab Med"}],"container-title":["Journal of Veterinary Internal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjvim.15381","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jvim.15381","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jvim.15381","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jvim\/article-pdf\/33\/2\/588\/66659156\/jvim15381.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jvim\/article-pdf\/33\/2\/588\/66659156\/jvim15381.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T03:32:20Z","timestamp":1770694340000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jvim\/article\/33\/2\/588\/8448049"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,1]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,3,1]]}},"URL":"https:\/\/doi.org\/10.1111\/jvim.15381","relation":{},"ISSN":["1939-1676"],"issn-type":[{"value":"1939-1676","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,3]]},"published":{"date-parts":[[2019,3,1]]},"assertion":[{"value":"2018-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}