{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T20:28:19Z","timestamp":1780086499500,"version":"3.54.0"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":2225,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T00:00:00Z","timestamp":1573603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Specific biomarkers of pyelonephritis (PN) in cats are lacking. MicroRNAs (miRNAs) have diagnostic potential in human nephropathies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate the presence\/stability of miRNAs in whole urine of cats and the discriminatory potential of selected urinary miRNAs for PN in cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Twelve healthy cats, 5 cats with PN, and 13 cats with chronic kidney disease (n = 5), subclinical bacteriuria (n = 3), and ureteral obstructions (n = 5) recruited from 2 companion animal hospitals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Prospective case-control study. Expression profiles of 24 miRNAs were performed by quantitative PCR (qPCR). Effect of storage temperature (4\u00b0C [24\u2009hours], \u221220\u00b0C, and \u221280\u00b0C) was determined for a subset of miRNAs in healthy cats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Urinary miR-4286, miR-30c, miR-204, miR4454, miR-21, miR-16, miR-191, and miR-30a were detected. For the majority of miRNAs tested, storage at 4\u00b0C and \u221220\u00b0C resulted in significantly lower miRNA yield compared to storage at \u221280\u00b0C (mean log2fold changes across miRNAs from \u22120.5 \u2009\u00b1\u20090.4 SD to \u22121.20\u2009\u00b1\u20090.4 SD (4\u00b0C versus \u221280\u00b0C) and from \u22120.7\u2009\u00b1\u20090.2 SD to \u22121.20\u2009\u00b1\u20090.3 SD (\u221220\u00b0C versus \u221280\u00b0C)). Cats with PN had significantly upregulated miR-16 with a mean log2fold change of 1.0\u2009\u00b1\u20090.4 SD, compared with controls (\u22120.1\u2009\u00b1\u20090.2, P = .01) and other urological conditions (0.6\u2009\u00b1\u20090.3, P = .04).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Upregulation of miR16 might be PN-specific, pathogen-specific (Escherichia coli), or both.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.15628","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T08:53:16Z","timestamp":1573635196000},"page":"166-175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Stability and profiling of urinary microRNAs in healthy cats and cats with pyelonephritis or other urological conditions"],"prefix":"10.1093","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9882-1637","authenticated-orcid":false,"given":"Lisbeth R.","family":"Jessen","sequence":"first","affiliation":[{"name":"University of Copenhagen Department of Veterinary Clinical Sciences, ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lise N.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"University of Copenhagen Department of Veterinary Clinical Sciences, ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida N.","family":"Kieler","sequence":"additional","affiliation":[{"name":"University of Copenhagen Department of Veterinary Clinical Sciences, ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7833-5191","authenticated-orcid":false,"given":"Rebecca","family":"Langhorn","sequence":"additional","affiliation":[{"name":"University of Copenhagen Department of Veterinary Clinical Sciences, ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bert J.","family":"Reezigt","sequence":"additional","affiliation":[{"name":"Blue Star Animal Hospital , Gothenburg,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Cirera","sequence":"additional","affiliation":[{"name":"University of Copenhagen Department of Veterinary and Animal Sciences, ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2026020302514937800_jvim15628-bib-0001","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1177\/1098612X16656910","article-title":"Renal pelvic and ureteral ultrasonographic characteristics of cats with chronic kidney disease in comparison with normal cats, and cats with pyelonephritis or ureteral obstruction","volume":"19","author":"Quimby","year":"2017","journal-title":"J Feline Med Surg"},{"key":"2026020302514937800_jvim15628-bib-0002","doi-asserted-by":"crossref","first-page":"932","DOI":"10.2460\/javma.2005.226.932","article-title":"Clinical, clinicopathologic, radiographic, and ultrasonographic abnormalities in cats with ureteral calculi: 163 cases (1984-2002)","volume":"226","author":"Kyles","year":"2005","journal-title":"J Am Vet Med Assoc"},{"key":"2026020302514937800_jvim15628-bib-0003","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1177\/1098612X12469522","article-title":"Urinary tract infections in cats with chronic kidney disease","volume":"15","author":"White","year":"2013","journal-title":"J Feline Med Surg"},{"key":"2026020302514937800_jvim15628-bib-0004","first-page":"186","article-title":"Pyelonephritis in Cats With Ureteral Obstruction: A Retrospective Study of 45 Cases. 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