{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T17:24:22Z","timestamp":1789406662209,"version":"build-2803163510"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":2104,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T00:00:00Z","timestamp":1586304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007235","name":"Colorado State University","doi-asserted-by":"publisher","award":["Center for 2016 Young Investigator Grant Program"],"award-info":[{"award-number":["Center for 2016 Young Investigator Grant Program"]}],"id":[{"id":"10.13039\/100007235","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007235","name":"Colorado State University","doi-asserted-by":"publisher","award":["Center for 2016 Young Investigator Grant Program"],"award-info":[{"award-number":["Center for 2016 Young Investigator Grant Program"]}],"id":[{"id":"10.13039\/100007235","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Concerns for recrudescence of Ehrlichia canis infection arise when immunosuppressive drugs are used to treat immune-mediated diseases in dogs previously infected with E. canis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Determine whether administration of prednisolone and cyclosporine would reactivate E. canis infection in dogs previously treated with doxycycline during the acute or subclinical phases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Seven beagles previously experimentally infected with E. canis and administered doxycycline for 4\u2009weeks were included. Three of the 7 dogs were incidentally concurrently infected with Anaplasma platys and Babesia vogeli and were administered 2 doses of imidocarb 2\u2009weeks apart before enrollment in the current study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Experimental study. Each dog was administered prednisolone and cyclosporine for 6\u2009weeks. Clinical signs, complete blood cell count (CBC), polymerase chain reaction (PCR) assays for E. canis, A. platys, and B. vogeli DNA in blood, E. canis indirect fluorescent antibodies (IFA) titers, and flow cytometry for antiplatelet antibodies were monitored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All dogs completed the immunosuppressive protocol. No evidence for recrudescence of E. canis, A. platys, or B. vogeli were detected based on clinical signs or results of CBC, PCR, IFA, and flow cytometry for antiplatelet antibodies. E. canis IFA titers were negative in 5\/7 dogs at the end of immunosuppressive protocol and were negative 6 months after the protocol in 5\/5 dogs available for testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Importance<\/jats:title>\n                    <jats:p>Dogs administered with a 4-week course of doxycycline with or without imidocarb failed to show evidence of activation of E. canis infection after administration of a commonly used immune suppressive protocol.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.15750","type":"journal-article","created":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T05:44:34Z","timestamp":1586324674000},"page":"1214-1221","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<i>Ehrlichia canis<\/i>\n                    in dogs experimentally infected, treated, and then immune suppressed during the acute or subclinical phases"],"prefix":"10.1093","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5611-4483","authenticated-orcid":false,"given":"Masahiko","family":"Sato","sequence":"first","affiliation":[{"name":"Colorado State University Department of Clinical Sciences, , Fort Collins, Colorado,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia K.","family":"Veir","sequence":"additional","affiliation":[{"name":"Colorado State University Department of Clinical Sciences, , Fort Collins, Colorado,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6472-5452","authenticated-orcid":false,"given":"Sarah B.","family":"Shropshire","sequence":"additional","affiliation":[{"name":"Colorado State University Department of Clinical Sciences, , Fort Collins, Colorado,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1605-9667","authenticated-orcid":false,"given":"Michael R.","family":"Lappin","sequence":"additional","affiliation":[{"name":"Colorado State University Department of Clinical Sciences, , Fort Collins, Colorado,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,5,1]]},"reference":[{"key":"2026020922030762300_jvim15750-bib-0001","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1128\/CMR.4.3.286","article-title":"The tribe Ehrlichieae and ehrlichial diseases","volume":"4","author":"Rikihisa","year":"1991","journal-title":"Clin Microbiol Rev"},{"key":"2026020922030762300_jvim15750-bib-0002","doi-asserted-by":"crossref","first-page":"937","DOI":"10.2460\/ajvr.1975.36.07.937","article-title":"Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus 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