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How chronic overfeeding affects development of these factors is poorly understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To examine the influence of long-term high-energy diet provision on body condition and ID.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Eleven Shetland pony mares.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In a 3-phase study, the high-energy group (n = 7) was fed 200% of net energy (NE) requirements (hay; concentrate: 36% sugar and starch, 13% fat) for 24\u2009weeks, followed by 17\u2009weeks hay-only feeding before resuming the high-energy diet (n = 4) for an additional 29\u2009weeks. Mares were weighed weekly. Oral glucose tolerance tests were performed 3 to 4 times per dietary period. Results were compared with those of a control group (phase 1, n = 4; phases 2 and 3, n = 6) that received 100% NE requirements, using a general linear mixed model with post hoc Bonferroni testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The mean body weight of the high-energy group increased by 27% per high-energy feeding period. During both feeding periods, area under the curve (AUC) for plasma glucose concentration decreased (P\u2009&amp;lt;\u2009.01), whereas AUC for plasma insulin concentration increased. Mean basal plasma glucose concentration and peak plasma insulin concentrations were higher (P\u2009&amp;lt;\u2009.05) in the high-energy group than in the control group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and Clinical Importance<\/jats:title>\n                    <jats:p>Feeding a high-energy diet to healthy nonobese Shetland pony mares led to more efficient glucose metabolism within 5\u2009weeks, followed by significant hyperinsulinemia and obesity. Hyperinsulinemic status was reversed during 17\u2009weeks of hay-only feeding, regardless of body condition, but returned rapidly after restarting the high-energy diet.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jvim.15788","type":"journal-article","created":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T11:15:35Z","timestamp":1588763735000},"page":"1339-1349","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of long-term overfeeding of a high-energy diet on glucose tolerance in Shetland pony mares"],"prefix":"10.1093","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7490-414X","authenticated-orcid":false,"given":"Nicky M. 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E.","family":"Gibson","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Utrecht University Department of Equine Sciences, , Utrecht,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"van Doorn","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Utrecht University Department of Equine Sciences, , Utrecht,","place":["The Netherlands"]},{"name":"Division of Nutrition , Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"de Ruijter-Villani","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Utrecht University Department of Equine Sciences, , Utrecht,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom A. 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